Recombinant Mouse Serine/threonine-protein kinase Chk1 (Chek1)

1. CHK1 expression levels control the timing of lymphomagenesis PMID: 29167438
2. During neurogenesis, cortical neurons became protected from S-phase Chk1 pathway activation by the DNA methyltransferase Dnmt1, and underwent cell death after S-phase progression. PMID: 28928282
3. Chk1(-/-) MEFs exhibited the absence of double-strand breaks, yet cells showed delayed DNA damage recovery with pan-nuclear immunostaining pattern of Histone H2AX. PMID: 28349497
4. RAD9 has a prominent role in the ATR-Chk1 pathway that is necessary for successful formation of the damage-sensing complex and DNA damage checkpoint signaling. PMID: 26667770
5. Acute inactivation of E4F1 in these cells results in CHK1-dependent checkpoint deficiency and multiple mitochondrial dysfunctions that lead to increased ROS production, energy stress, and inhibition of de novo pyrimidine synthesis PMID: 25843721
6. Altogether, our results classify E4F1 as a master regulator of CHK1 activity that ensures high fidelity of DNA replication, thus safeguarding genome stability. PMID: 25843717
7. Heterozygous loss of Chk1 in a Wnt-driven background resulted in an increase in DNA damage and apoptosis and accelerated both tumour development and progression. PMID: 24037525
8. PAR, supplied by PARP1, interacts with Chk1 via a novel PAR-binding regulatory (PbR) motif in Chk1, independent of ATR and its activity PMID: 24356582
9. High level of phospho-Chk1 is associated with skin tumors. PMID: 24184701
10. Chk1 is essential for the developmental formation of murine epidermal melanocytes but hemizygosity has little permanent developmental consequence in this cell type PMID: 23557358
11. activated Akt1 in Brca1-deficient cells impairs Chk1 nuclear localization and subsequently disrupts interaction of Chk1 and Rad51 leading to homologous recombination defects. PMID: 22665067
12. Our results uncover a novel p53-independent function for p21 in protecting cells from the lethal effects of DNA damage followed by Chk1 inhibition PMID: 22430210
13. The stress kinases p38 and JNK are activated when DNA replication is blocked and their activity allows S/M, but not G 2/M, checkpoint maintenance when Chk1 is inhibited. PMID: 22935704
14. Chk1 is indispensable for prophase I arrest and functions in G 2/M checkpoint regulation in meiotic oocytes. PMID: 22544319
15. hypoxia can activate p53 through inactivation of MDMX by the ATR-Chk1-MDMX-14-3-3gamma pathway. PMID: 22556425
16. complete loss of Chk1 is incompatible with epithelial tumorigenesis, whereas partial loss of function (haploinsufficiency) fosters benign malignant tumor progression. PMID: 21804609
17. an extra Chk1 allele prolongs the survival of ATR-Seckel mice, which suffer from high levels of replicative stress, but not that of ATM-deficient mice, which accumulate DNA breaks PMID: 22370720
18. genetic disruption of PP5 is associated with enhanced and prolonged phosphorylation of a single serine (Ser-345) on Chk1 PMID: 21921034
19. nuclear but not centrosomal Chk1 contributes to correct timing of mitotic entry. PMID: 21628425
20. Data show that checkpoint kinase-1 functions as a mitogen-dependent protein kinase that prevents premature differentiation of trophoblast stem cells by suppressing expression of p21 and p57, but not p27. PMID: 21791608
21. VIP blockade induces microcephaly through Mcph1 signaling; VIP/Mcph1/Chk1 signaling is key for normal cortical development PMID: 21737879
22. TAp73 induction by nitric oxide: regulation by checkpoint kinase 1 (CHK1) and protection against apoptosis. PMID: 21212274
23. Results suggest that the Chk1/2 double mutation leads to a high level of spontaneous DNA damage, but fails to eliminate cells with damaged DNA, which may ultimately increase cancer susceptibility independently of senescence. PMID: 20834228
24. data show that Chk1 is critical for maintaining genome integrity and serves as a double-edged sword for cancer: although its inhibition kills cancer cells, it also triggers tumorigenesis when favorable mutations are accumulated for cell growth PMID: 20473325
25. results support a role for Chk1 in maintaining the balance between erythroid progenitors and enucleated erythroid cells during differentiation PMID: 20052416
26. CHEK1 protein interacts with XIAP during mitosis. PMID: 14759516
27. Association of Fhit gene inactivation with increased survival after DNA damage, related to over-active checkpoints regulated by ATR/CHK1 pathway. Potential effects of Fhit-dependent DNA damage response on tumor progression. PMID: 15389587
28. Erythropoietin or IL-3 significantly enhanced etoposide-induced activation-specific phosphorylation of Chk1. PMID: 15674326
29. loss of PTEN and subsequent activation of AKT impair CHK1 through phosphorylation, ubiquitination, and reduced nuclear localization to promote genomic instability in tumor cells PMID: 15710331
30. These data demonstrate that lack of PTEN generates DNA damage due to inappropriate inactivation of CHK1.DNA damage due to the loss of PTEN is likely to stimulate tumor development. PMID: 15970699
31. Inhibition of PARP1 activity signals via a Chk1-dependent pathway for cell death by apoptosis. PMID: 16002346
32. inhibition of ataxia telangiectasia and Rad3 related and ataxia-telangiectasia mutated kinases with caffeine or Chk1 with UCN-01, results in activation of a p38-dependent intra-S-phase checkpoint and activation of apoptosis in ES cells PMID: 16138010
33. DSB repair from the complementary NHEJ pathway of PARP-1, but not SSB repair from the BER function of PARP-1, may play an essential role in the over-activated CHK1 regulated G2 checkpoint response and radiosensitivity in PARP-1-/- cells PMID: 17102615
34. Two-step phosphorylation of Chk1 at S317 and S345 appeared to be required for proper localization of Chk1 to centrosomes. PMID: 17242188
35. Chk1 is both critical for the survival of proliferating cells and engages in cross-talk with the Chk2 checkpoint kinase pathway PMID: 17360434
36. These findings show, for the first time, that TLR9 engagement on CD4 T cells reduces IR-induced apoptosis by influencing cell-cycle checkpoint activity. PMID: 18086870
37. Chk1 is a histone H3 threonine 11 kinase that regulates DNA damage-induced transcriptional repression. PMID: 18243098
38. Chk1 appears to help defend genomic integrity through effects on several other pathways, including Fanconi anemia proteins, the mitotic spindle, and transcription of cell cycle-related genes [review] PMID: 18424430
39. Chk1 is required for DNA replication at least through regulating RNR2 gene transcription. PMID: 18616928
40. Chk1 deficiency in the mouse small intestine results in p53-independent crypt death and subsequent intestinal compensation. PMID: 19169280
41. Chk1 is a multifunctional kinase that serves as a nexus between the DNA damage response and the mitotic exit pathways during cell-cycle progression to prevent genomic instability and cancer PMID: 19289837
42. Data show that Chk1 inhibitor PF-00477736 offers a therapeutic potential for the enhancement of taxane therapy. PMID: 19584159

Show More

Hide All

KEGG: mmu:12649

STRING: 10090.ENSMUSP00000034625

UniGene: Mm.16753