CHEK1, also known as CHK1 or OZEMA21, is a member of the Ser/Thr protein kinase family encoded by a human gene and is crucial for checkpoint regulation that halts the cell cycle in response to DNA damage or the presence of unreplicated DNA. CHEK1 integrates signals from ATM and ATR, two cell cycle proteins involved in the DNA damage response, which are also chromatin-associated during prophase I of meiosis. The phosphorylation of the CDC25A protein phosphatase by CHEK1 is necessary for delaying cell cycle progression in response to double-strand DNA breaks. CHEK1 is involved in DNA damage checkpoint signaling, the G2/M transition of the mitotic cell cycle, and the proliferation of inner cell mass cells. It functions in chromatin, the nucleus, and at replication forks and is expressed in various tissues, including adrenal medulla, early pregnancy, gonads, intestine, and trunk mesenchyme derived from the neural crest. Abnormal expression of CHEK1 is associated with various cancers, including pancreatic cancer.
CHEK1 Recombinant Monoclonal Antibody (CSB-RA176809A0HU)
Validated Data
Western Blot
Positive WB detected in: K562 whole cell lysate, 293T whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate
All lanes: Chk1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 55, 44, 51 kDa
Observed band size: 55 kDa
IHC image of CSB-RA176809A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of Hela Cells with CSB-RA176809A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
The following CHEK1 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
CHEK1 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA005338GA01HU | CHEK1 Antibody | Human,Mouse,Rat | ELISA,WB |
CSB-PA114140 | Phospho-CHEK1 (Ser280) Antibody | Human | ELISA,WB |
CSB-PA245196 | CHEK1 (Ab-317) Antibody | Human,Rat | ELISA,WB,IHC |
CSB-PA064953 | Phospho-CHEK1 (Ser286) Antibody | Human | ELISA,WB |
CSB-PA779402 | Phospho-CHEK1 (Ser301) Antibody | Human,Mouse,Rat | ELISA,WB |
CSB-PA980870 | Phospho-CHEK1 (Ser296) Antibody | Human | ELISA,WB,IHC |
CSB-PA584688 | CHEK1 (Ab-280) Antibody | Human | ELISA,WB |
CSB-PA001623 | CHEK1 Antibody | Human | WB, IHC, IF, ELISA |
CSB-PA001626 | CHEK1 Antibody | Human | WB, IHC, ELISA |
CSB-PA001629 | CHEK1 Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA006767 | Phospho-CHEK1 (S280) Antibody | Human | WB, ELISA |
CSB-PA006768 | Phospho-CHEK1 (S317) Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA006769 | Phospho-CHEK1 (S345) Antibody | Human,Mouse,Rat | IHC, ELISA |
CSB-PA006770 | Phospho-CHEK1 (S286) Antibody | Human | WB, IF, ELISA |
CSB-PA006771 | Phospho-CHEK1 (S301) Antibody | Human,Mouse,Rat | WB, IF, ELISA |
CSB-PA006772 | Phospho-CHEK1 (S296) Antibody | Human | WB, IHC, ELISA |
CSB-PA006773 | CHEK1 Antibody | Human | WB, ELISA |
CSB-PA006774 | CHEK1 Antibody | Human,Mouse,Rat | WB, IF, ELISA |
CSB-PA006775 | CHEK1 Antibody | Human,Mouse,Rat | WB, IHC, IF, ELISA |
CSB-PA927149 | CHEK1 Antibody | Human,Mouse,Rat | ELISA,WB |
CSB-PA005338LA01HU | CHEK1 Antibody | Human | ELISA |
CSB-PA005338LB01HU | CHEK1 Antibody, HRP conjugated | Human | ELISA |
CSB-RA176809A0HU | CHEK1 Recombinant Monoclonal Antibody | Human | ELISA, WB, IHC, IF |
CHEK1 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP005338HU CSB-EP005338HU CSB-BP005338HU CSB-MP005338HU CSB-EP005338HU-B |
Recombinant Human Serine/threonine-protein kinase Chk1 (CHEK1) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CHEK1 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP005338MO CSB-EP005338MO CSB-BP005338MO CSB-MP005338MO CSB-EP005338MO-B |
Recombinant Mouse Serine/threonine-protein kinase Chk1 (Chek1) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CHEK1 Proteins for Xenopus laevis (African clawed frog)
Code | Product Name | Source |
---|---|---|
CSB-YP738530XBE CSB-EP738530XBE CSB-BP738530XBE CSB-MP738530XBE CSB-EP738530XBE-B |
Recombinant Xenopus laevis Serine/threonine-protein kinase Chk1 (chek1) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CHEK1 Proteins for Gallus gallus (Chicken)
Code | Product Name | Source |
---|---|---|
CSB-YP806796CH CSB-EP806796CH CSB-BP806796CH CSB-MP806796CH CSB-EP806796CH-B |
Recombinant Chicken Serine/threonine-protein kinase Chk1 (CHEK1) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CHEK1 Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP846015RA CSB-MP846015RA CSB-EP846015RA-B |
Recombinant Rat Serine/threonine-protein kinase Chk1 (Chek1) | Yeast Mammalian cell In Vivo Biotinylation in E.coli |