Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Atp1a1

Uniprot No.

Q8VDN2

Research Area

Signal Transduction

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

341-775aa

Target Protein Sequence

TVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTENQSGVSFDKTSATWFALSRIAGLCNRAVFQANQENLPILKRAVAGDASESALLKCIEVCCGSVMEMREKYSKIVEIPFNSTNKYQLSIHKNPNASEPKHLLVMKGAPERILDRCSSILLHGKEQPLDEELKDAFQNAYLELGGLGERVLGFCHLLLPDEQFPEGFQFDTDDVNFPVDNLCFVGLISMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVNQVNPRDAKACVVHGSDLKDMTSEELDDILRYHTEIVFARTSPQQKLIIVEGCQRQGAIVAVTGDGVNDSPALKKADIGVAMGIVGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKS
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

53.3 kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Target Background

Function

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

Gene References into Functions

An antibody against the extracellular DR region (897-911) of Na(+)-K(+)-ATPase subunit alpha 1 disrupted the Na+-K+-ATPase/ROS amplifier and protected embryonic hearts in organ culture from ROS-induced injury and apoptosis. PMID: 28181111
Cardiotonic steroids activate NF-kappaB leading to proinflammatory cytokine production in primary macrophages through a signaling complex, including CD36, TLR4, and Na/K-ATPase. PMID: 28619997
Study identified the Na+/K+-ATPase alpha 1 and 3 subunits as receptors for the extracellular fragment of GPNMB that mediates activation of cellular signaling pathways and subsequent neuroprotective effects. PMID: 26988030
effects of endogenous acetylcholine on alveolar fluid clearance are likely mediated by Na,K-ATPase function through activation of muscarinic acetylcholine receptors on alveolar epithelia PMID: 26134533
Oxidized LDL-bound CD36 recruits an Na/K-ATPase-Lyn complex in macrophages that promotes atherosclerosis. PMID: 26350901
TGFbeta1 is a powerful regulator of megakaryocytic Na+/K+ ATPase activity. PMID: 25168590
Inhibition of the Na+/K+-ATPase pathway in astrocytes leads to rearrangement of cytoskeleton. PMID: 24095695
Reduction of Na/K-ATPase affects cardiac remodeling and increases c-kit cell abundance in partial nephrectomized mice. PMID: 24748592
Enhanced Na+ entry did not alter Na,K-ATPase a1 mRNA level in tranfected mCDD cells. Post-transcriptional control of Na,K-ATPase abundance is effected by lysosomal degradation. PMID: 24179170
This coupling of astrocytic A2ARs to the regulation of glutamate transport through modulation of NKA-alpha2 activity provides a novel mechanism linking neuronal activity to ion homeostasis controlling glutamatergic activity. PMID: 24259572
Concurrent impairment of Na(+)+K(+)-ATPase function in multi-organ may serve as one of the molecular pathways participating in and contributing to the mechanism of type-1 diabetes-induced complications in NOD mice. PMID: 22906552
Activation of Na,K-ATPase protects against oxidative damage in an animal model of pentylenetetrazol-induced seizures. PMID: 23219656
The results reveled that the NKAalpha1 subunit is found within alpha-motoneurons of spinal cord. PMID: 23761886
Reduction of Na+, K+-ATPase activity and expression in cerebral cortex of glutaryl-CoA dehydrogenase deficient mice is a possible mechanism for brain injury in glutaric aciduria type I. PMID: 22999741
Results indicate that Activation of Na+,K+-ATPase in skeletal muscle cells is AMPK-dependent. PMID: 22610379
the sodium pump alpha1 sub-unit has a role in progression of metastatic melanoma PMID: 19243476
ATP1A1 interaction with PKCeta and occludin was involved in the integrity of the blood-labyrinth-barrier PMID: 21304972
Both protein and mRNA expression of alpha1 and alpha2 isoforms of Na,K-ATPase and NKCC1 in the lateral wall were dramatically reduced following a long-term deafening PMID: 21094218
Functional coupling between the vascular alpha2 Na(+) pump and NCX1 is a critical molecular mechanism for salt-induced blood pressure elevation. (review) PMID: 21048395
Konjac polysaccharide could decrease body weight, postprandial blood glucose, serum leptin and intestinal mucosa Na -K+-ATPase activity of mice treated with high fat. PMID: 19408668
Alpha(1) isoform is expressed at more constant levels in both diaphragm and hindlimb muscles. PMID: 12397396
Mouse embryos homozygous for a null mutation in the Na/K-ATPase alpha1-subunit gene are able to undergo compaction and cavitation PMID: 15147760
results demonstrate that the Na,K-ATPase alpha 1 isoform regulates cardiac contractility and is functionally and physically coupled with the Na/Ca exchanger in heart PMID: 15485817
identification of groups of genes whose expressions were perturbed in the alpha1 heterozygous hearts compared to wild-type PMID: 15706699
The results indicate that the Na(+)/K(+)-ATPase is a potent regulator of tight junction formation and function during mouse preimplantation development. PMID: 16356488
identified a well conserved Na,K-ATPase catalytic alpha-subunit motif that binds to the inositol 1,4,5-trisphosphate receptor and can trigger an anti-apoptotic calcium signal. PMID: 16723354
Tubuloglomerular feedback requires activity of the alpha1 Na,K-ATPase, presumably in the macula densa. Furthermore, the data show that neither NHE2 nor colonic H,K-ATPase is essential for initiation of TGF responses[Na,K-ATPase alpha1] PMID: 16870707
while ATP1A3-isoforms regulate sodium and potassium homeostasis in subicular interneurones, ATP1A1-isoforms assume this function in pyramidal cells PMID: 17947306
This study provides a new link between alteration of sodium pump activity and hypertension in vivo, and suggests that TCTP might be a therapeutic target for the treatment of hypertension. PMID: 17976639
studies of the behavioral aspects of alpha1, alpha2, and alpha 3 deficient mice show that haploinsufficiency of both alpha2 and alpha 3 isoforms result in behavioral defects. PMID: 18044013
Examine roles of NCX1 and Atp1a1 in phospholamban regulation of cardiomyocyte contractility. PMID: 18708446

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Subcellular Location

Basolateral cell membrane; Multi-pass membrane protein. Cell membrane, sarcolemma; Multi-pass membrane protein. Cell projection, axon. Melanosome.

Protein Families

Cation transport ATPase (P-type) (TC 3.A.3) family, Type IIC subfamily

Database Links

KEGG: mmu:11928

STRING: 10090.ENSMUSP00000039657

UniGene: Mm.193670

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Code	CSB-EP851831MO
MSDS	MSDS
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse Sodium/potassium-transporting ATPase subunit alpha-1 (Atp1a1), partial

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