Product Details

Purity

Greater than 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Fully active measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). Plau needs to be activated by Plasmin to be enzymatically active. The specific activity is above 2000 pmol/min/ug.

Target Names

Plau

Uniprot No.

P06869

Alternative Names

Urokinase-type plasminogen activator; U-plasminogen activator; uPA; EC 3.4.21.73 [Cleaved into: Urokinase-type plasminogen activator long chain A; Urokinase-type plasminogen activator short chain A; Urokinase-type plasminogen activator chain B]

Species

Mus musculus (Mouse)

Source

Mammalian cell

Expression Region

21-433aa

Target Protein Sequence

GSVLGAPDESNCGCQNGGVCVSYKYFSRIRRCSCPRKFQGEHCEIDASKTCYHGNGDSYRGKANTDTKGRPCLAWNAPAVLQKPYNAHRPDAISLGLGKHNYCRNPDNQKRPWCYVQIGLRQFVQECMVHDCSLSKKPSSSVDQQGFQCGQKALRPRFKIVGGEFTEVENQPWFAAIYQKNKGGSPPSFKCGGSLISPCWVASAAHCFIQLPKKENYVVYLGQSKESSYNPGEMKFEVEQLILHEYYREDSLAYHNDIALLKIRTSTGQCAQPSRSIQTICLPPRFTDAPFGSDCEITGFGKESESDYLYPKNLKMSVVKLVSHEQCMQPHYYGSEINYKMLCAADPEWKTDSCKGDSGGPLICNIEGRPTLSGIVSWGRGCAEKNKPGVYTRVSHFLDWIQSHIGEEKGLAF

Mol. Weight

47.4 kDa

Protein Length

Full Length of Mature Protein

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Urokinase-type plasminogen activator plays a central role in extracellular matrix remodeling and cancer cell invasion, making its enzymatic characterization essential for understanding tumor progression mechanisms. This recombinant mouse Plau, spanning the full mature protein sequence (aa 21–433), demonstrates robust catalytic function with a specific activity exceeding 2000 pmol/min/μg against the fluorogenic substrate Z-GGR-AMC following plasmin activation, providing quantitative performance data that supports kinetic parameter analysis (Km, Vmax, kcat/Km) and inhibitor screening with reliable IC50 determination. The C-terminal 10×His tag preserves access to the catalytic domain while enabling straightforward purification, and the >95% purity by SDS-PAGE meets the quality thresholds commonly required for accurate enzymatic assays and structural studies where contaminants would confound kinetic measurements or crystallization trials. With endotoxin levels below 1.0 EU/μg, this preparation is appropriate for drug candidate evaluation and serves as a validated positive control in enzyme-linked assays investigating plasminogen activation pathways.

Target Background

Function

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

Gene References into Functions

downregulation of uPA could decrease the fertility of male mice, which may be caused by a reduction in sperm motility. PMID: 28298247
data presented here reveal important information about dynamics in uPA by demonstrating how various ligands can modulate uPA activity by mediating long-range conformational changes PMID: 29420634
Lymphangioleiomyomatosis is fatal lung disease associated with germline/somatic inactivating mutations in TSC2 genes. Data suggest lung lymphangioleiomyomatosis lesions and renal angiomyolipomas overexpress uPA; Tsc2-/- (or Tsc1-/-) embryonic fibroblasts from knockout mice express higher uPA levels than wild-type cells. (uPA = urokinase-type plasminogen activator; Tsc = tuberous sclerosis complex protein) PMID: 28972182
The findings suggest that the absence of uPA correlates with increased levels of Runx transcriptional regulators in a way that promotes inflammation-associated carcinogenesis. PMID: 29107070
an intricate link between caveolin-1 and Src kinase-mediated cell signaling and alveolar epithelial cell apoptosis due to loss of SP-C expression through p53 and uPA system-mediated cross-talk, is reported. PMID: 28385810
These studies identify uPA-dependent de-repression of vegfr1 and vegfr2 gene transcription through binding to HHEX/PRH as a novel mechanism by which uPA mediates the pro-angiogenic effects of VEGF and identifies a potential new target for control of pathologic angiogenesis. PMID: 27151212
Significance of the urokinase-type plasminogen activator and its receptor in the progression of focal segmental glomerulosclerosis in clinical and mouse models. PMID: 26846181
GM-CSF and uPA are required for Porphyromonas gingivalis-induced alveolar bone loss in a mouse periodontitis model. PMID: 25753270
Plau deficiency does not worsen controlled cortical impact-induced brain pathology or epileptogenesis caused by TBI. PMID: 26253597
we have firstly shown a fundamental mechanism of urokinase system(uPa and uPAR)-dependent regulation of the trajectory of growth and branching of blood vessels in early embryogenesis and in adults during the repair/regeneration of tissues. PMID: 26863767
Pharmacological inhibition of either uPA or selected MMPs decreased atherosclerosis in transgenic uPA mice. PMID: 25616415
Porphyromonas gingivalis-derived RgpA-Kgp complex activates the macrophage uPA. PMID: 25979345
transgenic alphaMUPA mice, with genetically extended life span, showed a much slower age-related decline in the rate of wound healing than their wild-type counterparts PMID: 25960543
Binding of urokinase to urokinase plasminogen activator receptor promotes dendritic spine recovery and functional outcome after ischemic stroke. PMID: 25339736
our results suggest a role for uPA and uPAR in experimental autoimmune encephalomyelitis pathogenesis PMID: 24120085
Urokinase plasminogen activator is a central regulator of macrophage three-dimensional invasion, matrix degradation, and adhesion. PMID: 24616477
SERPINE2 and PLAU are involved in cumulus expansion and oocyte maturation. PMID: 24023701
Data suggest urokinase-type plasminogen activator (uPA) is involved in susceptibility/resistance to Group A streptococcal infection (GAS); mice expressing human plasminogen transgene are more susceptible to invasive GAS than uPA knockout mice. PMID: 23853591
Mice deficient in urokinase-type plasminogen activator have delayed healing of tympanic membrane perforations. PMID: 23236466
PLAU is particularly important for memory Tregs and PLAU mediates Treg suppressor function via STAT5 and ERK signaling pathways. PMID: 23169000
The data showed that myeloid-derived suppressor cell accumulation is tumor burden-dependent, and that tumor-derived factors such as plasminogen activator urokinase and its receptor play a role in their recruitment. PMID: 23060546
The study establishes the 37- and 70-loops as a unique site for binding to compounds stabilizing the active state of serine proteases. PMID: 22950516
Over-expression of uPA plays a synergistic role in the development of liver injury, inflammation and regeneration during acute HBV infection PMID: 22563169
Data presented here suggest that MMP-9 is secreted as a multiprotein complex by T. gondii infected macrophages, similar to that observed in metastatic cells. PMID: 22177333
u-PA and plasminogen are essential for the phagocytosis of cellular debris by macrophages during liver repair PMID: 22318286
Expression of ALDH1A2, BEX2, EGR2, CCL3 and PLAU are upregulated in Toxoplasma gondiisusceptible C57BL/6 mice. PMID: 22451728
suggest that uPA promotes cell migration by binding to fibulin-5, initiating its cleavage by plasmin, which leads to its dissociation from beta1-integrin and thereby unblocks the capacity of integrin to facilitate cell motility PMID: 22280367
The uPA-uPAR axis has no effect on the formation of Ang II-induced abdominal aortic aneurysms, but uPA deficiency promotes aneurysmal rupture. PMID: 21868698
Urokinase type plasminogen activator, at concentrations found under pathological conditions, reduced pulmonary arterial contractility and increased permeability though the activation of NMDA-R1. PMID: 21617202
uPA acts in a cell signaling manner via binding to its receptor uPAR rather than as a protease. PMID: 21573723
contributes to heterogeneity of macrophages at the border of damaged site during liver repair PMID: 21301782
mechanisms of uPA/uPAR/plasminogen-accelerated atherosclerosis and aneurysm formation PMID: 21536666
The interaction of sM6P/IGF2R with Plg may be an important regulatory mechanism to inhibit migration of cells using the uPA/uPAR system. PMID: 21273553
We propose that u-PA has a protective role in arthritis models with 'wound healing-like' processes following local trauma PMID: 20973954
Early experimental venous thrombus resolution is independent of uPA. PMID: 20886179
study investigated whether host uPA is a crucial protagonist for the TIMP-1-induced modulation of a pro-metastatic microenvironment in the liver PMID: 19863693
uPA/uPAR downregulation suppresses angiogenesis in endothelial cells induced by glioblastoma cell lines PMID: 20805979
Selective abrogation of the uPA-uPAR interaction is associated with suppression of fibrin-associated inflammation. PMID: 20466854
u-PA is required for the full development of systemic arthritis models involving immune complex formation and deposition. PMID: 20196869
uPA plays a critical role in BV-2 microglial cell migration by activating pro-MMP-9, in part by its direct action on MMP-9 and also in part by the activation of plasminogen/plasmin cascade. PMID: 20177776
uPA deficiency can unfavorably modulate both delayed neurodegeneration and neurogenesis but has little effect on post-injury neuronal migration and vascular density. Elevated uPA during the post-injury phase is neuroprotective. PMID: 20026272
MAPK pathways ERK, JNK/SAPK, and P38-MAPK represent a significant component in the regulation of u-PA expression in human prostate cancer. PMID: 11676474
During infection with Cryptococcus neoformans, mice genetically deficient in uPA have a decreased number of recruited effector T cells in the lung, fail to generate a protective T1 profile of cytokines, and display impaired antifungal activity. PMID: 11777975
u-PA-/- mice developed significantly milder disease compared with the relevant wild-type mice PMID: 11891190
fibrinolysis by uPA-dependent plasmin activity plays a fundamental role in skeletal muscle regeneration PMID: 11929773
that u-PA plays important roles in liver regeneration after hepatectomy through control of a transcription factor, c-jun expression. PMID: 11983447
the role of urokinase in the regulation of monocyte/macrophage functions, such as that occurring in inflammatory reactions PMID: 12183060
uPA regulated by heregulin in mouse neoplasms PMID: 12209601
inducible expression reduces fibrosis and mortality after lung injury in mice PMID: 12376355
These results suggest that increasing uPA expression by an appropriate combination of growth factors and ECM components constitutes a possible approach to improving the migration of myogenic cells after transplantation. PMID: 12413883

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Subcellular Location

Secreted.

Protein Families

Peptidase S1 family

Database Links

KEGG: mmu:18792

STRING: 10090.ENSMUSP00000022368

UniGene: Mm.4183

Code	CSB-MP018121MO
Abbreviation	Recombinant Mouse Plau protein (Active)
MSDS	MSDS Datasheet
Size	$118
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Fully active measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). Plau needs to be activated by Plasmin to be enzymatically active. The specific activity is above 2000 pmol/min/ug. Biological Activity Assay
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Recombinant Mouse Urokinase-type plasminogen activator (Plau) (Active)

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