Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Prkg1

Uniprot No.

P0C605

Research Area

Cardiovascular

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

2-671aa

Target Protein Sequence

SELEEDFAKILMLKEERIKELEKRLSEKEEEIQELKRKLHKCQSVLPVPSTHIGPRTTRAQGISAEPQTYRSFHDLRQAFRKFTKSERSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYGKDSCIIKEGDVGSLVYVMEDGKVEVTKEGVKLCTMGPGKVFGELAILYNCTRTATVKTLVNVKLWAIDRQCFQTIMMRTGLIKHTEYMEFLKSVPTFQSLPDEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGQVNVTREDSPSEDPVFLRTLGKGDWFGEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKAYEDAEAKAKYEAEAAFFANLKLSDFNIIDTLGVGGFGRVELVQLKSEESKTFAMKILKKRHIVDTRQQEHIRSEKQIMQGAHSDFIVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYTACVVEAFAYLHSKGIIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISADYWSLGILMYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKIAKNAANLIKKLCRDNPSERLGNLKNGVKDIQKHKWFEGFNWEGLRKGTLTPPIIPSVASPTDTSNFDSFPEDSDEPPPDDNSGWDIDF
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

83.7 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Target Background

Function

Serine/threonine protein kinase that acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellular proteins. Numerous protein targets for PRKG1 phosphorylation are implicated in modulating cellular calcium, but the contribution of each of these targets may vary substantially among cell types. Proteins that are phosphorylated by PRKG1 regulate platelet activation and adhesion, smooth muscle contraction, cardiac function, gene expression, feedback of the NO-signaling pathway, and other processes involved in several aspects of the CNS like axon guidance, hippocampal and cerebellar learning, circadian rhythm and nociception. Smooth muscle relaxation is mediated through lowering of intracellular free calcium, by desensitization of contractile proteins to calcium, and by decrease in the contractile state of smooth muscle or in platelet activation. Regulates intracellular calcium levels via several pathways: phosphorylates IRAG1 and inhibits IP3-induced Ca(2+) release from intracellular stores, phosphorylation of KCNMA1 (BKCa) channels decreases intracellular Ca(2+) levels, which leads to increased opening of this channel. PRKG1 phosphorylates the canonical transient receptor potential channel (TRPC) family which inactivates the associated inward calcium current. Another mode of action of NO/cGMP/PKGI signaling involves PKGI-mediated inactivation of the Ras homolog gene family member A (RhoA). Phosphorylation of RHOA by PRKG1 blocks the action of this protein in myriad processes: regulation of RHOA translocation; decreasing contraction; controlling vesicle trafficking, reduction of myosin light chain phosphorylation resulting in vasorelaxation. Activation of PRKG1 by NO signaling alters also gene expression in a number of tissues. In smooth muscle cells, increased cGMP and PRKG1 activity influence expression of smooth muscle-specific contractile proteins, levels of proteins in the NO/cGMP signaling pathway, down-regulation of the matrix proteins osteopontin and thrombospondin-1 to limit smooth muscle cell migration and phenotype. Regulates vasodilator-stimulated phosphoprotein (VASP) functions in platelets and smooth muscle.

Gene References into Functions

These data thus demonstrate the existence of two differently distributed cGKI isoforms in the dorsal root ganglion, and may provide insight into the cellular and molecular mechanisms of pain. PMID: 30152261
This study demonstrates that H- ras deletion protects against AngII-induced cardiac remodeling, possibly via a mechanism in which PKG-Ibeta overexpression could play a partial role, and points to H-Ras and/or downstream proteins as potential therapeutic targets in cardiovascular disease. PMID: 29054855
that PKG-I expressed in nociceptors is not only a key determinant of dorsal root ganglion hyperexcitability and spinal synaptic plasticity but also an important modulator of cortical neuronal activity in pathological pain states PMID: 28326941
analysis of the role of binding interface with protein kinase G-Ialpha leucine zipper in vascular function PMID: 28280239
Brown adipose tissue, via Nox4-derived hydrogen peroxide, induces cyclic GMP-dependent protein kinase G type-1alpha activation, resulting in reduced vascular contractility. PMID: 28062507
disulfide formation at Cys(43) does not directly activate PKGIalpha, and the C43S-mutant PKGIalpha has a higher Ka for cGMP. Results highlight that mutant enzymes should be carefully biochemically characterized before making in vivo inferences. PMID: 28360102
PKG Ialpha disulfide formation triggers cardiac injury, and this initiation of maladaptive signaling can be blocked by pharmacological therapies that elevate cGMP, which binds kinase to limit its oxidation. PMID: 27342776
Cyclic GMP kinase deletion causes iron deficiency anemia, duodenal ulcers, and bleeding in the knockout mice. PMID: 26830212
Protein kinase G has a role in regulating production of H2S, which governs oxygen sensing PMID: 25900831
these results demonstrate that myocardial PKG1alpha oxidation prevents a beneficial response to pathological stress, may explain variable responses to PKG1alpha pathway stimulation in heart disease PMID: 25938783
Data show that cGMP-dependent kinase (cGK-Ialpha) knockdown partially rescued TNF-alpha-induced decrease in 42-kDa cGMP-dependent protein kinase-anchoring protein (GKAP42) and impairment of insulin signals. PMID: 25586176
miR-20a expression is up-regulated in response to hypoxia in both mouse and human pulmonary arterial smooth muscle cells PMID: 25447536
These findings characterize PKG as a novel regulator of AR-mediated transcription by enhancing AR cofactor p44/WDR77's function. PMID: 23755100
A functional leucine zipper domain in PKG-1alpha is essential for maintenance of a low pulmonary vascular tone in normoxia. PMID: 25128522
For the initial phase of angiotenin II-induced cardiac hypertrophy, lack of cardiomyocyte cGKI activity does not worsen hypertrophic growth. PMID: 25139994
the key role of PKG-I in transforming growth factor beta1 induction by like weak inducer of apoptosis in kidney cells PMID: 24872318
Results indicate that cGMP, acting primarily through cGKIalpha, is an important suppressor of kidney fibrosis. PMID: 23760283
cGKI and Trpc1,3,6 channels are not functionally coupled in vascular smooth cell. PMID: 23832809
Increasing PKG activity ameliorates renal fibrosis in part through regulation of macrophage and tubular cell function, leading to reduced TGF-beta-induced fibrosis. PMID: 24573388
cGMP, through activation of PKGI, inhibits c-Abl, leading to increased key antioxidant enzymes and resistance to lung endothelial oxidant injury. PMID: 24401847
In dystrophic hearts, excess contractility and arrhythmia are coupled to TRPC6 and are ameliorated by its targeted suppression or PKG activation. PMID: 24449818
PKG-1alpha dimerization is a major contributing factor to the vasodilator actions of DHEA. PMID: 24375799
mutation of the PKGIalpha LZ domain produces a clinically relevant model for hypertensive heart disease of aging. PMID: 23657971
ANP attenuates the inflammatory actions of histamine via endothelial GC-A/cGMP/cGKI signaling and inhibitory phosphorylation of TRPC6 channels. PMID: 23814119
PKG positively regulates proteasome activities and proteasome-mediated degradation of misfolded proteins, likely through posttranslational modifications to proteasome subunits. PMID: 23770744
Data indicate that oxidative activation of PKG Ialpha is a key mediator of hypotension and consequential organ injury during sepsis. PMID: 23716652
RhoA is a PKGIalpha target and direct binding of activated PKGIalpha to RhoA is central to cGMP-mediated inhibition of the VSMC Rho kinase contractile pathway PMID: 23066013
Data indicate that the 2 mechanisms (cGMP binding and the disulfide homodimer) of activating PKG1alpha are intricately linked with the binding of cGMP preventing oxidation to the disulfide state. PMID: 23006734
PKG-I deficiency induces pulmonary hypertension through Rho A/Rho kinase activation-mediated vasoconstriction and pulmonary vascular remodeling. PMID: 22632818
PKG-I-mediated presynaptic facilitation and synaptic long-term potentiation in spinal projection neurons is functionally involved in activity-dependent centrally mediated nociceptive hypersensitivity. PMID: 22427743
these findings suggest that cGK-Ialpha interacts with and phosphorylates rhotekin, thereby contributing to neurite outgrowth regulation. PMID: 22503686
Data show that protection of the duodenum from acid injury requires neuronal cGMP kinase I. PMID: 22253479
Sodium depletion enhances renal expression of (pro)renin receptor via cyclic GMP-protein kinase G signaling pathway. PMID: 22203739
role in noise-induced hearing loss and endogenous signaling pathway and activation of poly (ADP-ribose) polymerase PMID: 22270721
different signaling pathways exist for each cGKI isoform in vivo in fundus muscles PMID: 21914444
There are sex differences in the effects of PKG-I activation on the regulation of adipose tissue function and diet-induced obesity. PMID: 20930715
Genetic deletion of cGKI in non-neuronal cells results in a complex metabolic phenotype, including liver inflammation and fasting hyperglycemia. PMID: 21464444
protein kinase G type-I is essential for promoting proliferation and cell survival of mouse bone marrow stromal cells PMID: 21328456
Basal/moderately elevated PKG activity protects against high/pathological-level nitric oxide-induced apoptosis and promotes DNA synthesis/proliferation in vascular smooth muscle cells. PMID: 20060325
cGKI modulates glucagon release by suppression of calcium in alpha cells in mice. PMID: 20978093
Ang II suppression by ANP was abolished in cardiomyocytes of mice deficient in GC-A, in cyclic GMP-dependent protein kinase I (PKG I) or in the regulator of G protein signalling (RGS) 2, a target of PKG I PMID: 20352235
cGMP/cGKI signaling differentially inhibits contraction: in jejunum, inhibition is performed without changing [Ca(2+)](i) and is dependent on phosphatase activity, whereas in colon, inhibition is mediated by inhibition of [Ca(2+)](i) signals. PMID: 19628652
Cyclis GMP, through PKGI, attenuated H(2)O(2)-induced cytotoxicity in lung microvascular endothelial cells by increasing catalase and Gpx-1 expression through an unknown posttranscriptional effect. PMID: 20453163
evidence for PKGIalpha-dependent phosphorylation and activation of neuronal AAAD in vitro, and introduce AAAD as a putative PKGIalpha substrate. PMID: 20456015
These data establish that glucose-mediated downregulation of PKG levels stimulates TSP1 expression and enhances TGF-beta activity and matrix protein expression, which can contribute to vascular remodeling in diabetes. PMID: 20164378
Activation and inhibition of protein kinase G (PKG) respectively upregulates and downregulates recombinant potassium channel subfamily K (TASK1) channels heterologously expressed in PKG-loaded human embryonic kidney cells in vitro. PMID: 20410120
cardiac myocyte cGKI does not affect the development of heart hypertrophy induced by pressure overload or chronic isoproterenol infusion PMID: 20212138
(D)-Amino acid analogues of DT-2 as highly selective and superior inhibitors of cGMP-dependent protein kinase Ialpha. PMID: 20018259
PKGI links NO and cGMP signaling with the RhoA-ROCK and the insulin pathways, thereby controlling induction of adipogenic and thermogenic programs during brown fat cell differentiation PMID: 19952371
Cytosolic cGKI can support fear memory consolidation and LTP in neurons of the lateral amygdala via activation of CREB and CRE-dependent transcription. PMID: 20171263

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Subcellular Location

Cytoplasm.

Protein Families

Protein kinase superfamily, AGC Ser/Thr protein kinase family, cGMP subfamily

Tissue Specificity

Detected in cerebellum, hippocampus, dorsomedial hypothalamus, medulla, subcommissural organ, cerebral cortex, amygdala, habenulae, various hypothalamic regions, olfactory bulb, pituitary gland, and retina. Isoform alpha is prominent in the cerebellum and

Database Links

KEGG: mmu:19091

UniGene: Mm.381170

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Code	CSB-EP018715MO
MSDS	MSDS
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse cGMP-dependent protein kinase 1 (Prkg1)

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