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Recombinant Mycobacterium paratuberculosis Probable transcriptional regulatory protein MAP_1030 (MAP_1030)

Recombinant Mycobacterium paratuberculosis Probable transcriptional regulatory protein MAP_1030 (MAP_1030)

^{In Stock}

Code	CSB-EP352276MLA
Abbreviation	Recombinant Mycobacterium paratuberculosis MAP_1030 protein
MSDS	MSDS Datasheet
Size	$388
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Image	Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP352276MLA could indicate that this peptide derived from E.coli-expressed Mycobacterium paratuberculosis (strain ATCC BAA-968 / K-10) MAP_1030. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP352276MLA could indicate that this peptide derived from E.coli-expressed Mycobacterium paratuberculosis (strain ATCC BAA-968 / K-10) MAP_1030. (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Target Background

Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

MAP_1030

Uniprot No.

P62039

Research Area

Others

Alternative Names

MAP_1030; Probable transcriptional regulatory protein MAP_1030

Species

Mycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)

Source

E.coli

Expression Region

1-250aa

Target Protein Sequence

MSGHSKWATTKHKKAVIDARRGKMFARLIKNIEVAARVGGGDPAGNPTLYDAIQKAKKSSVPNENIERARKRGAGEEAGGADWQTITYEGYAPNGVAVLIECLTDNRNRAASEVRVAMTRNGGTMADPGSVSYLFSRKSVVTCEKNGLTEDDILAAVLDAGAEEVEDLGDSFEIICEPTDLVAVRTALQDAGIDYDSAEAGFQPSVTVPLNADGAQKVMRLVDALEDSDDVQDVWTNADIPDEILAQIEE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

32.8 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Tris-based buffer,50% glycerol

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

In the general approach to express the recombinant Mycobacterium paratuberculosis (strain ATCC BAA-968 / K-10) MAP_1030 protein, a plasmid encoding the Mycobacterium paratuberculosis (strain ATCC BAA-968 / K-10) MAP_1030 protein (1-250aa) is first constructed. The constructed plasmid is then introduced into e.coli cells. Plasmid-containing e.coli cells are screened and cultured under conditions that induce the protein expression. The protein is fused with a N-terminal 10xHis tag. Lysing the cultured cells and purifying the resulting recombinant MAP_1030 protein through affinity purification. The SDS-PAGE analysis is conducted to confirm the presence of the recombinant MAP_1030 protein and assess its purity. Its purity is over 85%.