Recombinant Pig Cathepsin B(CTSB)

Code CSB-YP006185PI
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Source Yeast
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Code CSB-EP006185PI
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Source E.coli
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Code CSB-EP006185PI-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006185PI
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Source Baculovirus
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Code CSB-MP006185PI
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names CTSB
Uniprot No. A1E295
Alternative Names CTSBCathepsin B; EC 3.4.22.1) [Cleaved into: Cathepsin B light chain; Cathepsin B heavy chain]
Species Sus scrofa (Pig)
Expression Region 80-332
Target Protein Sequence L PKSFDAREQW PNCPTIKEIR DQGSCGSCWA FGAVEAISDR ICIRSNGRVN VEVSAEDMLT CCGDECGDGC NGGFPSGAWN FWTKKGLVSG GLYDSHVGCR PYSIPPCEHH VNGSRPPCTG EGDTPKCSKI CEPGYTPSYK EDKHFGCSSY SISRNEKEIM AEIYKNGPVE GAFTVYSDFL QYKSGVYQHV TGDLMGGHAI RILGWGVENG TPYWLVGNSW NTDWGDNGFF KILRGQDHCG IESEIVAGIP CT
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis (By similarity).
Gene References into Functions
  1. Findings showed that CTSB facilitates adipogenesis, and its effect is associated with fibronectin degradation and Wnt/b-catenin signaling pathway. PMID: 24878992
  2. CTSB may be required to remodel endometrial and placental tissues for close apposition between maternal and fetal vasculatures and to facilitate transplacental transport of gases, micronutrients (amino acids, glucose), and macromolecules (proteins). PMID: 20107207
Subcellular Location Lysosome, Melanosome, Secreted, extracellular space
Protein Families Peptidase C1 family
Tissue Specificity Expressed in heart (at protein level).
Database Links

KEGG: ssc:100037961

STRING: 9823.ENSSSCP00000024395

UniGene: Ssc.53773

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