Recombinant Pig Insulin (INS)

Code CSB-YP011742PI
MSDS
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Source Yeast
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Code CSB-EP011742PI
MSDS
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Source E.coli
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Code CSB-EP011742PI-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP011742PI
MSDS
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Source Baculovirus
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Code CSB-MP011742PI
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
INS
Uniprot No.
Alternative Names
INS; Insulin [Cleaved into: Insulin B chain; Insulin A chain]
Species
Sus scrofa (Pig)
Expression Region
25-54
Target Protein Sequence
FVNQHL CGSHLVEALY LVCGERGFFY TPKA
Protein Length
Cytoplasmic domain
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Gene References into Functions
  1. We expect that these insulin-deficient pigs can be used in diabetes research to test the efficacy and safety of new drugs and the recipient of islet transplantation to investigate optimal transplantation strategies. PMID: 29691708
  2. Study used well-tempered bias exchange metadynamics simulations to determine the equilibrium ensembles of an insulin molecule under amyloidogenic conditions of low pH and high temperature. The folded state of a single insulin molecule was shown to be the most stable, longest-lived state even under amyloidogenic conditions. PMID: 28445749
  3. The findings are consistent with previous studies that indicate a link between Na,K-ATPase activity and SFK signaling. PMID: 27748508
  4. PTPLAD1 and AMPK are rapidly compartmentalized within the plasma membrane (PM) and Golgi/endosome fractions after insulin stimulation and that ATIC later accumulates in the Golgi/endosome fraction. PMID: 25687571
  5. Pdx-1, MafA and NeuroD1 bind to the A, C and E elements in the insulin promoter and regulate the transcriptional activity of the insulin promoter. PMID: 24825179
  6. The interplay of the adiponectin system, TNFalpha and insulin at a transcriptional level and, their effects on the adipogenic transcription factor PPARgamma, as well as on the activation of main insulin signaling pathways, is reported. PMID: 24846393
  7. Thermodynamics of insulin unfolding have been quantified by differential scanning calorimetry and thermal unfolding measurements to determine the extent and nature of their stabilization of the insulin hexamer. PMID: 24811232
  8. Exposing the hydrophobic core of insulin can induce the increase of amyloidogenicity and formation of higher-order polymerized fibrils, which is less toxic to membranes. PMID: 24041697
  9. Data suggest that a mutation in INS (C94Y) results a transgenic disease model for the investigation of permanent neonatal diabetes. PMID: 23274907
  10. The results show that modulation of plasma insulin levels by dietary carbohydrates seems possible in anabolic sows, but IGF-I levels are less easily modified. PMID: 21635573
  11. insulin increased GCLc promoter activity, which required a prerequisite increase or decrease in medium glucose PMID: 21871559
  12. SOCS3 is an important negative regulator of insulin signaling in porcine adipocytes. PMID: 20683642
  13. Plasma concentrations of insulin in pigs fed once per day were lower before feeding than after the meal. Plasma concentrations of insulin in ad libitum fed pigs exhibited random fluctuations. PMID: 20399063
  14. Data show that an expression cassette containing 1500bp of the porcine insulin promoter 5' UTR confers robust and specific transgene expression to beta-cells in vitro and in transgenic mice. PMID: 19682540
  15. Neutron diffraction data for T(6) porcine insulin were collected to 2.1 A resolution from a single crystal partly deuterated by exchange of mother liquor. PMID: 19770501
  16. computational analysis of the altered ionization of the B13 Glu in insulin B9 and B10 mutants of 4INS PMID: 15326283
  17. Results describe the in vitro folding/unfolding of insulin/single-chain insulin. PMID: 16800793
  18. A two-stage in vitro folding pathway of insulin is proposed, with six major folding intermediates captured during the folding process. PMID: 17914645
  19. Streptozotocin is able to impair in vitro neonatal pancreatic beta cell insulin release whereas human cytokines is not. PMID: 17991146
  20. A16Leu had much more significant effects on the foldability of insulin than B17Leu. PMID: 18071885
  21. These data demonstrated that feed allowance level has important consequence on embryo survival, embryo development and uterine development, possibly mediated by changes in concentrations of reproductive hormones. PMID: 18700853
  22. Functional surfaces of insulin were probed by photocross-linking of an extensive set of azido derivatives constructed by chemical synthesis. PMID: 19321435

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Subcellular Location
Secreted.
Protein Families
Insulin family
Database Links
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