Pig Insulin,INS ELISA Kit

Code CSB-E06828p
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P01315
Lead Time 3-5 working days
Protein Biological Process 2 glyconeogenesis and glycometabolism
Abbreviation INS
Protein Biological Process 1 Biosynthesis/Metabolism
Target Name insulin
Alias ILPR, IRDN, OTTHUMP00000011162|OTTHUMP00000196038|proinsulin
Species Sus scrofa (Pig)
Protein Biological Process 3 Carbohydrate metabolism
Sample Types serum, plasma, tissue homogenates
Detection Range 2 μIU/mL-400 μIU/mL
Sensitivity 1 μIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Target Details After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5 region and with the IGF2 gene at the 3 region.
HGNC 3818
RGD 61807
MGI 1347487
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 96  
Range % 84-105  
1:2 Average % 93  
Range % 89-99  
1:4 Average % 95  
Range % 89-101  
1:8 Average % 95  
Range % 83-103  
Recovery
The recovery of pig INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 91-101  
EDTA plasma (n=4) 98 92-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μIU/ml OD1 OD2 Average    
0 1.800 1.864 1.832    
2 1.569 1.581 1.575    
8 1.304 1.327 1.316    
32 0.812 0.805 0.809    
100 0.389 0.393 0.391    
400 0.180 0.183 0.182    
References
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Function Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Subcellular Location Secreted
Protein Families Insulin family
Database Links

KEGG: ssc:397415

STRING: 9823.ENSSSCP00000025428

UniGene: Ssc.583

Pathway HIF-1 signaling pathway
MAPK signaling pathway
mTOR signaling pathway
PI3K-Akt signaling pathway
Regulation of actin cytoskeleton
Autophagy
Ras signaling pathway
AMPK signaling
cGMP-PKG signaling pathway
FoxO signaling pathway
Insulin signaling pathway
Phospholipase D signaling pathway
Rap1 signaling pathway

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