Pig Insulin,INS ELISA Kit

Pig Insulin,INS ELISA Kit

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Specification

Target

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INS Proteins

INS Proteins

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Pig Insulin,INS ELISA Kit

Specification
Target

Code

CSB-E06828p

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Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

P01315

Lead Time

3-5 working days

Protein Biological Process 2

glyconeogenesis and glycometabolism

Abbreviation

INS

Protein Biological Process 1

Biosynthesis/Metabolism

Target Name

insulin

Alias

ILPR, IRDN, OTTHUMP00000011162|OTTHUMP00000196038|proinsulin

Species

Sus scrofa (Pig)

Protein Biological Process 3

Carbohydrate metabolism

Sample Types

serum, plasma, tissue homogenates

Detection Range

2 μIU/mL-400 μIU/mL

Sensitivity

1 μIU/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Competitive

Target Details

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5 region and with the IGF2 gene at the 3 region.

HGNC

3818

RGD

61807

MGI

1347487

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	84-105
1:2	Average %	93
	Range %	89-99
1:4	Average %	95
	Range %	89-101
1:8	Average %	95
	Range %	83-103

Recovery

The recovery of pig INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-101
EDTA plasma (n=4)	98	92-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μIU/ml	OD1	OD2	Average
0	1.800	1.864	1.832
2	1.569	1.581	1.575
8	1.304	1.327	1.316
32	0.812	0.805	0.809
100	0.389	0.393	0.391
400	0.180	0.183	0.182

References

Alteration of muscle fiber characteristics and the AMPK-SIRT1-PGC-1伪 axis in skeletal muscle of growing pigs fed low-protein diets with varying branched Duan Y.et al,Oncotarget,Dec 5
Branched-chain amino acid ratios modulate lipid metabolism in adipose tissues of growing pigs Yehui Duan.et al,Journal of Functional Foods,Jan
β‐hydroxy‐β‐methyl butyrate promotes leucine metabolism and improves muscle fibre composition in growing pigs Yehui H. Duan.et al,Animal physiology and animal nutrition,July 15
Preparation and characterization of insulin-loaded zein/carboxymethylated short-chain amylose complex nanoparticles Na Ji.et al,journal of agricultural and food chemistry,August 15

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Function	Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Subcellular Location	Secreted
Protein Families	Insulin family
Database Links	KEGG: ssc:397415 STRING: 9823.ENSSSCP00000025428 UniGene: Ssc.583
Pathway	HIF-1 signaling pathway MAPK signaling pathway mTOR signaling pathway PI3K-Akt signaling pathway Regulation of actin cytoskeleton Autophagy Ras signaling pathway AMPK signaling cGMP-PKG signaling pathway FoxO signaling pathway Insulin signaling pathway Phospholipase D signaling pathway Rap1 signaling pathway

INS Protein

INS Proteins for Sus scrofa (Pig)

Code	Product Name	Source
CSB-YP011742PI CSB-EP011742PI CSB-BP011742PI CSB-MP011742PI	Recombinant Pig Insulin(INS)	Yeast E.coli Baculovirus Mammalian cell

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Check Code：

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.