Recombinant Pig Osteopontin (SPP1)

Code CSB-YP022603PI
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP022603PI
MSDS
Size Pls inquire
Source E.coli
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Code CSB-EP022603PI-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP022603PI
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP022603PI
MSDS
Size Pls inquire
Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SPP1
Uniprot No.
Alternative Names
SPP1; OPN; Osteopontin; Bone sialoprotein 1; Secreted phosphoprotein 1; SPP-1
Species
Sus scrofa (Pig)
Expression Region
17-303
Target Protein Sequence
LPVK QTNSGSSEEK LLSNKYTDAV ATLLKPDPSQ KQTFLAPQNT ISSEETDDFK QETLPSKSNE SPEQTDDVDD DDDEDHVDSR DTDSEEADHA DDADRSDESH HSDESDELVT DFPTDTPATD VTPAVPTGDP NDGRGDSVVY GLRSKSKKFR RSEAQQLDAT EEDLTSHVES EETDGTPKAI LVAQRLHVAS DLDSQEKDSQ ETSQPDDRSV ETRSQEQSKE YTIKTYDGSN EHSNVIESQE NPKVSQEFHS HEDKLVPDSK SEEDKHLKLR VSHELESASS EIN
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.; Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Gene References into Functions
  1. These data suggested that methylation in the OPN promoter plays a crucial role in the regulation of OPN expression that we found in cloned pigs genome. PMID: 25101273
  2. The striking breed differences between hyperprolific Large White and Meishanin pigs of secreted phospoprotein 1 expression in endometrium suggest that SPP1 may be associated with placental efficiency. PMID: 23575146
  3. Microglia incubated in vitro with different concentrations (0.1 fM-1 nM) of recombinant osteopontin showed increased proliferation at 10 fM. PMID: 22974008
  4. results of this study reveal faster growth rate and differences in pig productivity according to genotypes of the SPP1 gene PMID: 22391653
  5. Osteopontin could play an important role in the development of neointimal hyperplasia in venous conduits after coronary artery bypass grafting. PMID: 22219474
  6. A different MSSCP pattern was shown in osteopontin which indicates that mutation is located in promotor region; the A --> G transitions was identified in two positions -617 and -608 PMID: 20010486
  7. The 3' terminal end of the first intron of porcine SPP1 harbors a C/EBPbeta binding site and this binding site is negatively affected by the mutant G allele. PMID: 19845957
  8. in pregnant pigs SPP1 is induced by conceptus estrogen in uterine luminal epithelium and is regulated in a manner coincident with placental progesterone production PMID: 16120824
  9. osteopontin promotes pathologic mineralization via calcium pyrophosphate dihydrate crystal formation in articular cartilage. PMID: 17123806
  10. Osteopontin is detected in the majority of germ cells and is involved in spermatogenesis in boar testis. PMID: 17519561

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Subcellular Location
Secreted.
Protein Families
Osteopontin family
Database Links
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