Recombinant Polycystin-2 (pkd-2), partial

Code CSB-YP883324CXY1
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP883324CXY1
MSDS
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Source E.coli
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Code CSB-EP883324CXY1-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP883324CXY1
MSDS
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Source Baculovirus
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Code CSB-MP883324CXY1
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
pkd-2
Uniprot No.
Alternative Names
pkd-2; pdk-2; Y73F8A.1; Polycystin-2; CePc2; Polycystic kidney disease 2 protein homolog
Species
Caenorhabditis elegans
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Functions as a calcium permeable cation channel. Required for 2 aspects of male mating behavior: response to hermaphrodite contact and vulva location. Acts in the same pathway as lov-1 and atp-2 in response behavior.
Gene References into Functions
  1. acts as a major calcium-release channel in the endoplasmic reticulum in cells where rapid calcium signaling is required, and is essential in those excitable cells for rapid responses to stimuli (polycystin-2) PMID: 15862350
  2. Both general and cell-type-specific factors govern TRPP2/PKD-2 subcellular distribution by forming at least two steps involving somatodendritic and ciliary sorting decisions. PMID: 16943275
  3. Results demonstrate that somatodendritic and ciliary targeting of PKD-2 requires the transmembrane region of PKD-2 and that the PKD-2 cytosolic termini regulate subcellular distribution and function. PMID: 18037411
  4. 11 mutants found with defects in the ciliary localization (cil) of C. elegans PKD-2, a transient receptor potential polycystin (TRPP) channel PMID: 18407554
Subcellular Location
Cell membrane; Multi-pass membrane protein. Cell projection, cilium membrane. Cell projection, cilium. Cell projection, axon. Cell projection, dendrite. Perikaryon. Endoplasmic reticulum membrane.
Protein Families
Polycystin family
Tissue Specificity
Exclusively expressed in a subset of 3 categories of adult male sensory neurons: ray neurons, hook neurons and head cephalic (CEM) neurons. Expressed in the male tail.
Database Links

KEGG: cel:CELE_Y73F8A.1

STRING: 6239.Y73F8A.1

UniGene: Cel.12535

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