Recombinant Rabbit Tissue factor pathway inhibitor (TFPI) (Active)

Tissue factor pathway inhibitor serves as a critical regulator of the extrinsic coagulation cascade, making it a key target in cardiovascular and thrombosis research. This recombinant rabbit TFPI, spanning the full mature sequence (aa 25–300) with an N-terminal 10×His tag positioned away from the functional Kunitz domains, demonstrated robust binding activity in a functional ELISA, with an EC50 of 2.281–3.783 ng/mL against an anti-TFPI recombinant antibody when immobilized at 1 μg/mL. These validated binding data support use in inhibitor screening and IC50 determination, enzymatic activity assays, substrate specificity profiling, and as a positive control in enzyme-linked assay formats. Mammalian-expressed to preserve native post-translational modifications, this protein meets the quality thresholds commonly required for kinetic parameter analysis and drug candidate evaluation, with purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/μg to minimize assay interference.