Product Details

Purity

>85% (SDS-PAGE)

Target Names

Pla2g6

Uniprot No.

P97570

Alternative Names

Pla2g6; Pnpla9; 85/88 kDa calcium-independent phospholipase A2; CaI-PLA2; EC 3.1.1.4; Group VI phospholipase A2; GVI PLA2; Intracellular membrane-associated calcium-independent phospholipase A2 beta; iPLA2-beta; Patatin-like phospholipase domain-containing protein 9; PNPLA9

Species

Rattus norvegicus (Rat)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Calcium-independent phospholipase involved in phospholipid remodeling with implications in cellular membrane homeostasis, mitochondrial integrity and signal transduction. Hydrolyzes the ester bond of the fatty acyl group attached at sn-1 or sn-2 position of phospholipids (phospholipase A1 and A2 activity respectively), producing lysophospholipids that are used in deacylation-reacylation cycles. Hydrolyzes both saturated and unsaturated long fatty acyl chains in various glycerophospholipid classes such as phosphatidylcholines, phosphatidylethanolamines and phosphatidates, with a preference for hydrolysis at sn-2 position. Can further hydrolyze lysophospholipids carrying saturated fatty acyl chains (lysophospholipase activity). Upon oxidative stress, contributes to remodeling of mitochondrial phospholipids in pancreatic beta cells, in a repair mechanism to reduce oxidized lipid content. Preferentially hydrolyzes oxidized polyunsaturated fatty acyl chains from cardiolipins, yielding monolysocardiolipins that can be reacylated with unoxidized fatty acyls to regenerate native cardiolipin species. Hydrolyzes oxidized glycerophosphoethanolamines present in pancreatic islets, releasing oxidized polyunsaturated fatty acids such as hydroxyeicosatetraenoates (HETEs). Has thioesterase activity toward fatty-acyl CoA releasing CoA-SH known to facilitate fatty acid transport and beta-oxidation in mitochondria particularly in skeletal muscle. Plays a role in regulation of membrane dynamics and homeostasis. Selectively hydrolyzes sn-2 arachidonoyl group in plasmalogen phospholipids, structural components of lipid rafts and myelin. Regulates F-actin polymerization at the pseudopods, which is required for both speed and directionality of MCP1/CCL2-induced monocyte chemotaxis. Targets membrane phospholipids to produce potent lipid signaling messengers. Generates lysophosphatidate (LPA, 1-acyl-glycerol-3-phosphate), which acts via G-protein receptors in various cell types. Has phospholipase A2 activity toward platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), likely playing a role in inactivation of this potent proinflammatory signaling lipid. In response to glucose, amplifies calcium influx in pancreatic beta cells to promote INS secretion.

Gene References into Functions

Data suggest glucose-induced insulin secretion is first up-regulated (acute infection), then down-regulated (chronic infection) in beta cells infected with S. aureus; Pla2g4a is up-regulated in acute infection; Pla2g6 is up-regulated in chronic infection. PMID: 26632509
membrane potential sensitive activity is the iPLA2gamma PMID: 26206229
Leptin was shown to significantly inhibit the activity of iPLA2, which is considered to play crucial roles in non-apoptotic cell death, resulting in effective prevention of ischemia-induced myocyte death. PMID: 25979360
the mitochondrial iPLA2 may help to coordinate local capillary blood flow with changing energy demands PMID: 24586040
acts as an inhibitory modulator of NKCC2 activity in thick ascending limb PMID: 22218592
cPLA(2) and iPLA(2) are involved in stimulating endothelial migration and proliferation in the presence of glioma conditioned culture medium. PMID: 21094175
These findings indicate that inhibition of iPLA(2) results in excessive exocytosis through increased oxidative damage (or failure to repair such damage) and defects in mitochondrial function. PMID: 21116712
iPLA2 is shown to have a regulatory role in the development of neuroinflammation through upregulation of divalent calcium ion signaling in the brain. PMID: 21178110
reactive oxygen species-induced function of cardiac mitochondrial-iPLA2 may stand on a pro-survival side of ischemia-reperfusion injury PMID: 20406040
iPLA(2) activation in response to acute ischemia in STZ-induced diabetic hearts is more pronounced than in control hearts and that this response is a significant contributor to arrhythmogenic conduction slowing. PMID: 21037228
The results suggest that glucocorticoids increase calcium concentrations in myocytes and stimulate iPLA(2)-dependent calcium entry and that steroid induced muscle protein degradation may be regulated by increased iPLA(2) activity and calcium metabolism. PMID: 20107037
Results provide evidence for endogenous proteolytic processing of iPLA(2)beta and redistribution of iPLA(2)beta variants in subcellular compartments. PMID: 20132906
Mitochondrial iPLA(2) plays a role in the remodeling of cardiolipin's acyl composition. PMID: 19741254
iPLA(2) plays a substantial role in the generation of endothelium-derived contracting factor evoked by acetylcholine. PMID: 20118407
The agonist-stimulated release of arachidonic acid (AA) from cellular phospholipids in many cell types (e.g. myocytes, beta-cells, and neurons) has been demonstrated to be primarily mediated by calcium-independent phospholipases A(2) (iPLA(2)s). PMID: 12089145
iPLA2 plays a role in thrombin-induced arachidonic acid release and growth in VSMC and these responses are mediated by p38 MAPK PMID: 12928445
Type VI iPLA2 drives the onset of acute pleurisy through the synthesis of PGE2, LTB4, PAF, and IL-1beta. PMID: 15003994
The GVIB iPLA2 is present in rat liver peroxisomes, organelles that synthesize plasmalogens and carry out fatty acid beta-oxidation. PMID: 15052324
The iPLA2 inhibitor bromoenol lactone, when introduced into hippocampal CA1 pyramidal cells, generated a dose-dependent increase in the amplitude of AMPA receptor-mediated EPSPs. PMID: 15132431
Activities of both enzymes increased upon their association, and iPLA(2)beta reaction products reduced CaMKIIbeta activity PMID: 15576376
mitochondrial Ca(2+)-independent phospholipase A(2) may be an important factor governing cell death caused by necrosis or apoptosis PMID: 16407316
These results cast doubt on the proposed mechanism involving iPLA2beta required for Ca2+ entry. PMID: 16854462
the PKCalpha-cPLA(2) pathway appears to play a key role in the early phase of EC-pericyte interactions regulating blood retina or blood-brain barrier maturation. PMID: 17267947
Thus, we identify a regulatory mechanism important for the brain, in which docosahexaenoic acid released from astrocytes by VIB Ca(2+)-independent phospholipase A(2) negatively controls prostaglandin production. PMID: 17555549
The phospholipase iPLA2 specifically associated with the endosomal and exosomal membranes could be activated by reactive oxygen species (ROSs) produced during mitochondria degeneration. PMID: 17666570
These results suggest an important role for iPLA2 in the cortex-striatum-thalamus-cortex circuitry. PMID: 17768607
mechanism of vasodilatation by urocortin involves the regulation of iPLA2 and SOCE via the stimulation of a cAMP/PKA-dependent signal transduction cascade in rat coronary artery. PMID: 17885217
Expression of iPLA(2)gamma or iPLA(2)beta induces cytoprotection against complement-dependent glomerular epithelial cell injury. PMID: 18171998
the iPLA(2)beta-ceramide axis plays a critical role in activating the mitochondrial apoptotic pathway in insulin-secreting cells during ER stress PMID: 18936091
a novel function of iPLA 2beta, related not to its phospholipase activity but to its thioesterase activity, which contributes to optimal fatty acid oxidation in skeletal muscle. PMID: 18937505

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Subcellular Location

Cytoplasm. Cell membrane. Mitochondrion. Cell projection, pseudopodium.

Tissue Specificity

Expressed in pancreatic beta-cells. Expressed in skeletal muscle (at protein level).

Database Links

KEGG: rno:360426

STRING: 10116.ENSRNOP00000017104

UniGene: Rn.44692

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Recombinant Rat 85 kDa calcium-independent phospholipase A2 (Pla2g6)

Product Details

Customer Reviews and Q&A

Target Background

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