Recombinant Rat Actin, cytoplasmic 1 (Actb)

Code CSB-YP353072RA
MSDS
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Source Yeast
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Code CSB-EP353072RA
MSDS
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Source E.coli
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Code CSB-EP353072RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP353072RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP353072RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Actb
Uniprot No.
Alternative Names
Actb; Actin; cytoplasmic 1; Beta-actin) [Cleaved into: Actin; cytoplasmic 1; N-terminally processed]
Species
Rattus norvegicus (Rat)
Expression Region
1-375
Target Protein Sequence
MDDDIAALVV DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGVMVGMGQK DSYVGDEAQS KRGILTLKYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE HPVLLTEAPL NPKANREKMT QIMFETFNTP AMYVAIQAVL SLYASGRTTG IVMDSGDGVT HTVPIYEGYA LPHAILRLDL AGRDLTDYLM KILTERGYSF TTTAEREIVR DIKEKLCYVA LDFEQEMATA ASSSSLEKSY ELPDGQVITI GNERFRCPEA LFQPSFLGME SCGIHETTFN SIMKCDVDIR KDLYANTVLS GGTTMYPGIA DRMQKEITAL APSTMKIKII APPERKYSVW IGGSILASLS TFQQMWISKQ EYDESGPSIV HRKCF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA.
Gene References into Functions
  1. Data show that spermiation is actin- and microtubule-based cytoskeletal-dependent event, involving regulatory proteins that modify cytoskeletal organization. PMID: 29040437
  2. Data show that spermatid/phagosome transport across the epithelium during spermatogenesis requires the coordination between actin- and microtubule (MT)-based cytoskeletons. PMID: 26894662
  3. Both ZBP1 and HuD bind the zipcode element in rat beta-actin mRNA's 3' UTR. Differences between HuD and ZBP1 were observed in their binding preference to the element. PMID: 26152301
  4. Our study demonstrates that gene expression levels of reference genes commonly used in obesity-related studies, such as ACTB or RPS18, are altered by changes in acute or chronic energy status. PMID: 23736358
  5. Axonal translation of beta-actin mRNA supports axon branching and axonal translation of GAP-43 mRNA supports elongating growth. PMID: 23426659
  6. The authors hypothesize a new post-transcriptional mechanism regulating a messenger encoded by nuclear genome for a mitochondrial protein and that Hsp90 and Actin, could represent key players in cytochrome c oxidase subunit IV translation. PMID: 19703590
  7. Axonal outgrowth seen after the second of two serial axotomies is related to the ability of newly synthesized actin that is already in transit to polymerize in response to the first axotomy. PMID: 12504890
  8. The molecular mechanism and function of beta-actin mRNA localization in dendrites of cultured hippocampal neurons was studied; ZBP1 is required for the localization of beta-actin mRNA to dendrites PMID: 14614102
  9. extremely high levels of beta-actin mRNA in osteoclasts observed in developing molar tooth germ in the mandibular bone surface PMID: 14756242

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Subcellular Location
Cytoplasm, cytoskeleton. Nucleus.
Protein Families
Actin family
Tissue Specificity
Expressed in the epididymis (at protein level).
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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