Recombinant Rat Guanine nucleotide-binding protein G(q) subunit alpha(Gnaq)

Code CSB-YP009594RA
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Source Yeast
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Code CSB-EP009594RA
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Source E.coli
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Code CSB-EP009594RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP009594RA
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Source Baculovirus
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Code CSB-MP009594RA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Gnaq
Uniprot No. P82471
Alternative Names GnaqGuanine nucleotide-binding protein G(q) subunit alpha; Guanine nucleotide-binding protein alpha-q
Species Rattus norvegicus (Rat)
Expression Region 1-359
Target Protein Sequence MTLESIMACC LSEEAKEARR INDEIERQLR RDKRDARREL KLLLLGTGES GKSTFIKQMR IIHGSGYSDE DKRGFTKLVY QNIFTAMQAM VRAMDTLKIP YKYEHNKAHA QLVREVDVEK VSAFENPYVD AIKSLWNDPG IQECYDRRRE YQLSDSTKYY LNDLDRVADP SYLPTQQDVL RVRVPTTGII EYPFDLQSVI FRMVDVGGQR SERRKWIHCF ENVTSIMFLV ALSEYDQVLV ESDNENRMEE SKALFRTIIT YPWFQNSSVI LFLNKKDLLE EKIMYSHLVD YFPEYDGPQR DAQAAREFIL KMFVDLNPDS DKIIYSHFTC ATDTENIRFV FAAVKDTILQ LNLKEYNLV
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Regulates B-cell selection and survival and is required to prevent B-cell-dependent autoimmunity. Regulates chemotaxis of BM-derived neutrophils and dendritic cells (in vitro). Transduces FFAR4 signaling in response to long-chain fatty acids (LCFAs).
Gene References into Functions
  1. Enhanced activation of protein kinase C delta (PKCdelta) in vascular smooth muscle cell (VSMC) from hypertensive rats (SHR) increases the expression of G alpha protein q (Gqalpha) and phospholipase C beta1 (PLCbeta1) proteins and results in VSMC hypertrophy. PMID: 27379421
  2. Enhanced oxidative stress in VSMCs from SHRs activates c-Src, growth factor receptors and MAPK signaling, contributing to enhanced expression of Gqalpha/PLCbeta1 proteins and VSMC hypertrophy. PMID: 26747500
  3. Galphaq protein carboxyl terminus imitation polypeptide GCIP-27 improves cardiac function in chronic heart failure rats. PMID: 25822412
  4. Calcium flow through the alpha7 channel and activation of the Galphaq pathway. PMID: 24687992
  5. Enhanced the expression of Gqalpha/PLC-beta1 proteins in vascular smooth muscle from 16-wk-old spontaneously hypertensive rats results in VSMC hypertrophy. PMID: 24760983
  6. Suggest the involvement of the Gnaq and phosphatidylinositol 3-kinase signaling cascades in mediating melatonin effects in pancreatic alpha-cells. PMID: 22672634
  7. Acute beta-adrenergic activation triggers nuclear import of histone deacetylase 5 and delays G(q)-induced transcriptional activation PMID: 23161540
  8. These studies show that caveolae play a direct and active role in regulating basal Ca(2+) activity in cardiomyocytes. PMID: 21463572
  9. Pre-treatment with propofol can reduce the concentration of Gq/11 protein in kidney tissue during acute respiratory distress syndrome. PMID: 17166350
  10. increased levels in vascular smooth muscle cells exposed to high glucose and in aortic vascular smooth muscle cells from diabetic rats PMID: 20691780
  11. C-terminal deletion of mGlu(1) receptor selectively abolishes Galpha(q/11) coupling. PMID: 19879871
  12. 9 aa in the N terminus of RGS8 contribute to the function to inhibit Gq-coupled signaling in a receptor type-specific manner and the regulatory function of RGS8S is especially diminished on Gq-coupled responses PMID: 12110731
  13. an Akt-mediated cell survival pathway is compromised by the diminished availability of PIP2 elicited by pathological levels of Gq activity in cardiomyocytes PMID: 12900409
  14. Results suggest a potential pathophysiological role of loss of fine tuning due to selective RGS2 down-regulation in G(q/11)-mediated remodeling in hypertrophic cardiomyocytes. PMID: 16380388
  15. increased Galphaq activity can provide anti-apoptotic signals by eliciting EGFR phosphorylation and subsequent Akt activation PMID: 16466740
  16. Inhibition of rat granulosa cell differentiation by overexpression of Galphaq. PMID: 18401763
  17. The carboxyl-terminus of a synthetic G alpha q peptide effectively attenuates left ventricular remodelling in spontaneously hypertensive rats; the anti-remodelling effect may not be dependent entirely on decreases in blood pressure. PMID: 18518877
  18. These findings suggest that G(q/11)-coupled receptors function as sensors of membrane stretch in VSM cells. PMID: 18987636

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Subcellular Location Cell membrane; Lipid-anchor. Golgi apparatus. Nucleus. Nucleus membrane.
Protein Families G-alpha family, G(q) subfamily
Database Links

KEGG: rno:81666

STRING: 10116.ENSRNOP00000019174

UniGene: Rn.22817

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