Product Details

Purity

≥ 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA.Immobilized Rat Lypd3 at 2 μg/mL can bind Anti-LYPD3 recombinant antibody(CSB-RA013263MA2HU). The EC50 is 3.515-3.992 ng/mL.

Target Names

Lypd3

Uniprot No.

O55162

Alternative Names

Ly6/PLAUR domain-containing protein 3; GPI-anchored metastasis-associated protein C4.4A; Lypd3; C4.4a

Species

Rattus norvegicus (Rat)

Source

Mammalian cell

Expression Region

33-330aa

Target Protein Sequence

LECYSCVQKADDGCSPHKMKTVKCGPGVDVCTEAVGAVESIHGQFSVAVRGCGSGIPGKNDRGLDLHGLLAFIQLQQCTEDRCNAKLNLTLRGLNPAGNESAYEHNGAECYSCMGLSREKCQGAMPPVVNCYNASGRVYKGCFDGNVTLTAANVTVSLPVRGCVQDEACTRDGVTGPGFTLSGSCCQGPRCNSDLRNKTYFSPRIPPLVLLPPPTTPAPSTRTQNSSSTTSTTAPTTATTTIKPTTVQASHTSSTHETEHEVIQEEGSHLSGGATGHQDRSNMGKFPEKGGAQIPSKG

Mol. Weight

32.6 kDa

Protein Length

Full Length of Mature Protein

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Lypd3, a GPI-anchored protein implicated in metastasis and tumor progression, presents challenges for functional studies due to its membrane-associated nature and complex disulfide-bonded structure. This recombinant rat Lypd3 (aa 33–330) demonstrates quantifiable binding activity with an EC50 of 3.515–3.992 ng/mL against an anti-LYPD3 antibody in functional ELISA, confirming proper folding and epitope accessibility that supports its use in antibody validation assays, receptor-ligand interaction studies, and as a positive control in binding screens. Mammalian cell expression preserves the native post-translational modifications critical for maintaining the structural integrity of this Ly6/PLAUR domain-containing protein. The protein meets the quality thresholds commonly required for cancer biology research, with >95% purity by SDS-PAGE and endotoxin levels <1.0 EU/μg, providing a suitable basis for cell-based functional assays investigating Lypd3's role in tumor cell migration and invasion.

Target Background

Function

Supports cell migration. May be involved in tumor progression.

Gene References into Functions

the first explanation for the C4.4A contribution to wound healing and metastasis. PMID: 22431918
a possible functional role of C4.4A in early differentiation of stratified squamous epithelia PMID: 21339181

Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor.

Tissue Specificity

Found predominantly on the basal layers of squamous epithelium. Expressed in the gravid uterus and on epithelial of the upper gastrointestinal tract. It has been found in tumor lines which metastasize via the lymphatic system.

Database Links

KEGG: rno:60378

STRING: 10116.ENSRNOP00000027100

UniGene: Rn.38434

Code	CSB-MP013263RA
Abbreviation	Recombinant Rat Lypd3 protein (Active)
MSDS	MSDS Datasheet
Size	$138
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA.Immobilized Rat Lypd3 at 2 μg/ml can bind Anti-LYPD3 recombinant antibody(CSB-RA013263MA2HU). The EC₅₀ is 3.515-3.992 ng/mL. Biological Activity Assay
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Recombinant Rat Ly6/PLAUR domain-containing protein 3 (Lypd3) (Active)

Product Details

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Target Background

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