Recombinant Rat Multidrug resistance-associated protein 1 (Abcc1), partial

Code CSB-YP804505RA
MSDS
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Source Yeast
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Code CSB-EP804505RA
MSDS
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Source E.coli
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Code CSB-EP804505RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP804505RA
MSDS
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Source Baculovirus
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Code CSB-MP804505RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Abcc1
Uniprot No.
Alternative Names
Abcc1; Mrp1; Multidrug resistance-associated protein 1; EC 7.6.2.2; ATP-binding cassette sub-family C member 1; Glutathione-S-conjugate-translocating ATPase ABCC1; EC 7.6.2.3; Leukotriene C(4) transporter; LTC4 transporter
Species
Rattus norvegicus (Rat)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs by decreasing accumulation of drug in cells, and by mediating ATP- and GSH-dependent drug export. Hydrolyzes ATP with low efficiency. Catalyzes the export of sphingosine 1-phosphate from mast cells independently of their degranulation. Participates in inflammatory response by allowing export of leukotriene C4 from leukotriene C4-synthezing cells.
Gene References into Functions
  1. The ROS-mediated increase in the synthesis of MRP-1 and BCRP can play an important role in hyperoxaluria-promoted calcium oxalate urolithiasis by facilitating phosphatidylserine redistribution in renal epithelial cells. PMID: 28577929
  2. The data of this study show increased Abcc1, Abcc2, and Abcc4 mRNA expression at the BBB in response to H/R stress and that Abcc gene expression is regulated by Nrf2 signaling. PMID: 28298215
  3. MRP1 is downregulated in glioma cells by chitosan-MRP1 siRNA nanoparticles PMID: 25543328
  4. Sonication enhanced sensitivity of glioma tumor cells to doxorubicin by down-regulating the expressions of p-glycoprotein and multidrug resistance protein 1. PMID: 23940624
  5. The number of MRP1-positive cells in the cortex and hippocampus was also significantly increased in amygdale kindling rats. PMID: 23474709
  6. Xifeng Capsule used alone or combined with carbamazepine can effectively inhibit MRP1 expression in hippocampus and cortex of epileptic rats. PMID: 22883408
  7. Results show that the presence of Mdr1a, Mdr1b (P-glycoprotein Pgp) and multidrug resistance-associated protein (MRP1) encoding genes in the olfactory mucosa of rat and mouse was confirmed by RT-PCR. PMID: 22563480
  8. In olfactory epithelium, high levels of expression of xenobiotic transporters Mrp1, Mrp3, Mrp5, Mdr1a, Oatp2 and Oat6 are expressed. PMID: 22015764
  9. ATP-binding cassette ABCC1 is involved in the release of sphingosine 1-phosphate from rat uterine leiomyoma ELT3 cells and late pregnant rat myometrium PMID: 21803151
  10. increased activity of MRP1 in diabetic glomeruli is correlated with an inadequate adaptive response to oxidative stress PMID: 21495913
  11. down-regulation of Mrp1 protein at the blood-cerebrospinal fluid barrier may impact the Mrp1-mediated neuroprotective functions of the blood-cerebrospinal fluid barrier and actually potentiate UCB neurotoxicity PMID: 21297965
  12. Hepatic oval cells express high levels of MDR1, MRP1 and Bcrp1 genes which may have cytoprotective functions during severe hepatotoxicity. PMID: 17669244
  13. gp120 and TNF-alpha can up-regulate Mrp1 expression in cultured astrocytes. PMID: 20051532
  14. Identification of multidrug resistance protein 1 (MRP1/ABCC1) as a molecular gate for cellular export of cobalamin. PMID: 19897579
  15. MRP1 is involved in olfaction as a part of the "olfactory signal termination" by the chemical homeostasis in the "perireceptor events" of the olfactory epithelium. PMID: 19879335
  16. polyclonal antibodies recognizing both human and rodent MRP1 PMID: 11689020
  17. Mrp1 participates in the release of glutathione from astroglial cells PMID: 12125073
  18. MRP1 cDNA has been cloned, characterized and the transfected protein found capable of efflux transport of a fluorescent MRP1 marker--calcein--that is inhibitable by known MRP1 inhibitors, indomethacin, and MK571. PMID: 12423064
  19. The upregulation of Mrp1 in hemolysis is mediated by unconjugated bilirubin and/or other products of heme oxygenase,supporting a role of Mrp1 in transport and protection from its cellular toxicity. PMID: 14623264
  20. rat Abcc1 did not significantly confer cellular resistance to anthracyclins of vinca alkaloids. PMID: 14641820
  21. Mrp1 was rather expressed than P-gp in parenchyma astrocytes and in glia limitans lining the meninges. PMID: 15328029
  22. Transfection of lymphocytes with MRP1 gene completely abolished cyclopentenone prostaglandins (CP-PG) (0-40 microM) toxicity. Our findings suggest that CP-PG accumulation in lymphocytes may be, at least partially, responsible for cancer immunodeficiency. PMID: 16170839
  23. intrinsic higher capacity to express the MRP1/GS-X pump may dictate the redox status in the heart muscle PMID: 16868918
  24. The mRNA induction of MDR1, MRP1, MRP2 and MRP3 by rifampicin (Rif), dexamethasone (Dex) and omeprazole (Ome) was investigated in primary cultures of cryopreserved human and rat hepatocytes. PMID: 16946557
  25. Transport of sphingosine-1-phosphate by ABCC1 influenced migration of mast cells toward antigen but not degranulation PMID: 17050692
  26. These results point to a novel role of Mrp1 in the susceptibility of premature babies to UCB encephalopathy, and provide a startup point for the development of a new therapeutic strategy. PMID: 17141959
  27. The MRP1 gene is present in primary cultured rat epithelial cells of both types I- and II-like phenotypes and its corresponding protein is localized in the basolateral membrane of these cells. PMID: 17854063
  28. ABCB1, but not ABCC1, activity was downregulated in peripheral blood mononuclear cells and kidney of spontaneously hypertensive rats. PMID: 18057958
  29. Exposing prostatic tumor cell line to cell hypoxia reduced the Mrp1 activity by 24%. PMID: 18290326
  30. Increased molecular expression and functional activity of Mrp1 is demonstrated in an in vitro model of an HIV-associated pathological condition. PMID: 18485102
  31. MRP-1 transporter and efflux at skeletal muscles possibly play significant roles in statin-induced myopathy PMID: 18509883
  32. Results compare the relative amounts of ABCb1 (Pgp) and ABCc1 (Mrp1)at the blood-brain and blood-cerebrospinal fluid barriers, located respectively at the brain capillary endothelium and the choroid plexus epithelium. PMID: 18680196

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
ABC transporter superfamily, ABCC family, Conjugate transporter (TC 3.A.1.208) subfamily
Tissue Specificity
Skeletal muscle, brain, heart, spleen, lung and kidney.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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