Recombinant Rat Muscarinic acetylcholine receptor M1(Chrm1),partial

Code CSB-YP005381RA
Size US$1593 How to order?
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names Chrm1
Uniprot No. P08482
Research Area Others
Alternative Names Chrm1; Chrm-1Muscarinic acetylcholine receptor M1
Species Rattus norvegicus (Rat)
Source Yeast
Expression Region 210-366aa
Target Protein Sequence RIYRETENRARELAALQGSETPGKGGGSSSSSERSQPGAEGSPESPPGRCCRCCRAPRLLQAYSWKEEEEEDEGSMESLTSSEGEEPGSEVVIKMPMVDSEAQAPTKQPPKSSPNTVKRPTKKGRDRGGKGQKPRGKEQLAKRKTFSLVKEKKAART
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 19.1kDa
Protein Length Cytoplasmic Domain
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
Gene References into Functions
  1. PKC delta activation modulates neurotrophins levels by a signaling pathway that involves M1R activation and ultimately leading to an increase in retinal ganglion cells survival in vitro. PMID: 29705702
  2. Results establish an intracellular pathway linking M1 receptors, inositol triphosphate receptors, and ubiquitin proteasome system activity to object memory destabilization and suggest a previously unacknowledged role for cholinergic signaling in long-term memory modification and storage. PMID: 29030298
  3. Study examined the expression of M1 muscarinic ACh receptors in the hippocampus at 7 and 30 days following experimental bilateral vestibular lesions (BVL) in rats using autoradiography. Analysis of the brain tissue revealed a significant reduction in the number of M1 receptors throughout the hippocampus and striatum at 30 days but not at 7 days following BVL. PMID: 27569857
  4. M1 and M3 muscarinic receptors may play a role in the neurotoxicity of anhydroecgonine methyl ester, a cocaine pyrolysis product. PMID: 26626425
  5. Our results suggest that the mechanism for the deficits in learning and memory of rats with chronic fluorosis may be associated with the decreased expressions of M1 and M3 in mAChRs PMID: 25417050
  6. Neonatal hypoglycemia decreased total muscarinic receptors (p < 0.001) with reduced muscarinic M1, M2, and M3 receptor genes (p < 0.001) in one month old rats. PMID: 25474381
  7. M1 cholinergic receptor activation in the central nervous system can modulate endotoxin tolerance in rat liver PMID: 25008070
  8. Selective M1 and M4 receptor activation each improved memory. PMID: 24480931
  9. Blockade of M1-cholinoreceptors does not prevent the inhibitory effect of carbacholine on contractility of the atrial and ventricular myocardium. PMID: 24288746
  10. M1 mAChR may have a role (via the PKC-ERK1/2 pathway) in the neuroprotective effects of lesatropane PMID: 23988164
  11. The results of this study findings suggested that M1R mechanisms in the BLa are complex, involving postsynaptic effects as well as regulating release of neurotransmitters from presynaptic terminals. PMID: 23559406
  12. There is a significant decrease in muscarinic M1 receptors in the brain stem following spinal cord injury. PMID: 23184186
  13. M1-mAChRs function at both surface and intracellular sites in telencephalon neurons including the hippocampus PMID: 23678982
  14. Muscarinic receptor expression is developmentally regulated; M1, M3, and M4 receptors are the main subtypes expressed in oligodendrocyte progenitor cells. PMID: 21913336
  15. Report therapeutic potential of the M(1) receptor positive allosteric modulator BQCA for the treatment of memory deficits observed in Alzheimer's disease. PMID: 23085025
  16. Data suggest that Chrm1 is down-regulated in cell membranes of parotid glands in a model of periodontitis and participates in potassium release; Chrm1 expression appears to return to control level upon indomethacin treatment of periodontitis. PMID: 22513211
  17. Muscarinic receptor activation determines the downstream effects of store-operated Ca(2+)-entry on neuronal membrane excitability and energy metabolism. PMID: 22088219
  18. Expression of muscarinic receptor M1, M3, M5 subunits decreased in the flocculus after unilateral labyrinthectomy. PMID: 19160866
  19. Use of scannning mutagenesis uncovers helix 8 of the M1 mAChR, and a G protein recognition site is delineated in the activated state of the receptor. PMID: 21247934
  20. study showed that M and M receptors, IP3 and cGMP were functionally regulated during diabetes as function of age PMID: 21054404
  21. These data indicate a novel mechanism of synaptic plasticity in which activation of M1 receptors results in AMPA receptor endocytosis, via a mechanism involving interactions between GluA2, GRIP and liprin-alpha. PMID: 19534762
  22. Chrm1 receptor is located on presynaptic glutamatergic terminals afferent to the recorded pyramidal neuron, thus decreasing glutamate release. PMID: 20600670
  23. muscarinic M(1) receptor showed a significant decrease and muscarinic M(3) receptor subtype showed a significant increased binding in the cerebral cortex of hypoglycemic rats compared to diabetic and control PMID: 21126518
  24. Cholinergic activation through M(1)-like receptor enhances basket-cell activity & promotes the generation of nested theta & gamma rhythms, enhancing hippocampal function and associated performance. PMID: 20433901
  25. Thus, the current study identifies ASIC1a as a novel target for muscarinic signaling. PMID: 19730136
  26. Acetylcholine through muscarinic M1 and M3 receptors stimulated calcium release from the pancreatic islets. PMID: 19554469
  27. The results indicate that muscarinic M(1) modulation of Ca(V)2.1 Ca(2+) channels in these neurons involves phosphoinositide hydrolysis. PMID: 19883739
  28. Results highlight long-term low dose somatotropin and insulin treatment in regulating muscarinic M1 and M3 cholinergic and NMAR1 and mGluR5 glutamate receptor subtypes in aging rats and rejuvenation of brain function. PMID: 19666081
  29. no change on muscarinicM1 mRNA expression was found in the hippocampus of isolation reared rats PMID: 12384226
  30. In the neuromuscular junction, the modulation of Ach release via M1 and M2 muscarinic autoreceptors differs between the developing and adult rat PMID: 12534975
  31. This represents the first demonstration of M1 receptors on neuronal progenitor cells and supports the notion that M1 muscarinic receptors may play a role in development of the nervous system PMID: 14675151
  32. muscarinic acetylcholine receptor-stimulated inositol 1,4,5-trisphosphate production is augmented by synaptic activity to facilitate Ca2+ release in hippocampal neurons PMID: 15342646
  33. central muscarinic M1 and M3 receptor subtypes functional balance is suggested to regulate sympathetic and parasympathetic activity, which in turn control the islet cell proliferation and glucose homeostasis PMID: 15350825
  34. M1 mAChR stimulation leads to GSK-3beta inactivation. Cross-talk between muscarinic signaling and Wnt components underlie neuroprotective effect of M1 mAChR activation. PMID: 15474371
  35. endogenous GRK2 is a key regulator of M1 mACh receptor signaling in hippocampal neurons PMID: 15743771
  36. Stimulation of M1 muscarinic acetylcholine receptors activates endogenous transient receptor potential-canonical subtype 6 (TRPC6) channels in neuronal PC12D cells. PMID: 15994335
  37. KCNQ channel currents in striatal medium spiny neurons were potently reduced by M1 muscarinic receptors. PMID: 16093396
  38. ductules and epididymis suggests that these receptors play a role in the modulation of luminal fluid composition and smooth muscle contraction. PMID: 16160857
  39. study shows that infection with low numbers of Hymenolepis diminuta leads to modulation of the cholinergic components of the myenteric plexus & M2 receptor mRNA & that large number of worms result in suppression in relative expression of M1 receptor mRNA PMID: 16541262
  40. results show that the dicyclomine did not affect IA and CFC consolidation, suggesting specific involvement of M1 muscarinic receptor only in acquisition these tasks, and these effects was not state-dependent PMID: 16647280
  41. Data suggest that acetylcholine acting on M1 and M2 acetylcholine receptors may contribute to selective excitability enhancement or depression in individual, rostrally projecting sensory neurons. PMID: 16820015
  42. Ach agonists inhibit the non-quantal secretion of ACh from motor nerve terminals by acting on post-synaptic M1 receptors coupled to Ca(2+) channels in the sarcolemma to induce sarcoplasmic Ca(2+)-dependent synthesis and the release of NO. PMID: 17561934
  43. The findings suggest that activation of M1-type muscarinic cholinergic receptors in the dorsomedial striatum facilitate the flexible shifting of response patterns by maintaining or learning a new choice pattern once selected. PMID: 17709264
  44. Four derivatives (5a-5c and 5e) showed considerable M1 receptor binding affinity (in vitro) and elicited beneficial effects in vivo memory and learning models (Rodent memory evaluation, plus and Y maze studies). PMID: 17719699
  45. Study suggests that formation of a direct hydrogen bond between the highly conserved side chains of Asp71 and Asn414 may be a critical feature stabilizing the activated state of the M1 muscarinic acetylcholine receptor. PMID: 17823120
  46. Taken together these data suggest that the level of synaptic activity may determine the different kinases recruited to regulate M1 mACh receptor desensitization in neurons. PMID: 17908240
  47. insulin modulates binding parameters and gene expression of total and muscarinic M1 receptors. PMID: 17960828
  48. Data show that L- and N-type calcium channel current, but not M-current inhibition by M1 muscarinic receptors requires DAG lipase activity. PMID: 18247369
  49. These results provide further evidence of the expression of the M(1) muscarinic receptor subtype in the rat heart PMID: 18443764
  50. Data suggest that 77-LH-28-1 is a potent, selective, bioavailable and brain-penetrant agonist at the M1 mAChR. PMID: 18454168

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Subcellular Location Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
Protein Families G-protein coupled receptor 1 family, Muscarinic acetylcholine receptor subfamily, CHRM1 sub-subfamily
Database Links

KEGG: rno:25229

STRING: 10116.ENSRNOP00000024785

UniGene: Rn.119395

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