Recombinant Rat Neprilysin(Mme),partial

Code CSB-YP362251RA
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Source Yeast
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Code CSB-EP362251RA
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Source E.coli
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Code CSB-EP362251RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP362251RA
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Source Baculovirus
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Code CSB-MP362251RA
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Source Mammalian cell
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Product Details

>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
MmeNeprilysin; EC; Atriopeptidase; Enkephalinase; Neutral endopeptidase 24.11; NEP; Neutral endopeptidase; Skin fibroblast elastase; SFE; CD antigen CD10
Rattus norvegicus (Rat)
Protein Length
Tag Info
The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Target Background

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of the atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
Gene References into Functions
  1. hypoxia increased the expression of platelet-derived growth factor (PDGF) and transforming growth factor-beta1 (TGF-beta1) and decreased the expression of neprilysin (NEP), which contributed to the hypoxia-induced Endothelial-to-mesenchymal transition of pulmonary artery endothelial cells. PMID: 27373199
  2. The data obtained suggest that the increase of caspase-3 enzyme activity could affect NEP expression via proteolytic degradation of its transcription factor AICD PMID: 26983277
  3. the increased expression of NPR-C (r=-0.943 and -0.837 for mRNA and protein respectively, p<0.05) and NEP (r=-0.687 and -0.823 for mRNA and protein respectively, p<0.05) were accompanied by a signi fi cant decline in CNP. PMID: 24189506
  4. Data suggest that the brain expression of neprilysin may promote amyloid beta-protein (Abeta)deposition in patients with Alzheimer's disease (AD). PMID: 24508800
  5. These results suggested that ketamine suppresses the Abeta degradation of NEP by reducing p38 MAPK-mediated pathway activity. PMID: 23624023
  6. Naoerkang can increase the expression of NEP in hippocampal tissues of AD rats so as to reduce the quantity of AAbeta(1-42). PMID: 20141738
  7. The three Abeta-degrading enzymes were damaged to different extents in the brain of diabetic rats, and impairment of ECE-1 and IDE partly contributed to the elevated Abeta(1-40) levels in brain of diabetic rats. PMID: 20414044
  8. Androgen regulation of amyloid beta (Abeta)protein involves an androgen receptor-dependent mechanism requiring up-regulation of Abeta catabolizing enzyme neprilysin. PMID: 18346198
  9. Ovariectomy increases BACE1 expression and decreases neprilysin expression in hippocampus. PMID: 20137687
  10. Intracardiac expression of neutral endopeptidase PMID: 12030369
  11. in spontaneously hypertensive rats, the local concentration and action of adrenomedullin in the tissues may be differentially regulated by NEP. PMID: 15005274
  12. Neutral endopeptidase inactivity induces an exaggerated neurogenic inflammation mediated by neurokinin-1 receptor, which may lead to edema in pulmonary alveoli. PMID: 15194566
  13. Report that CGS 35601 and its orally active prodrug CGS 37808 are triple inhibitors of endothelin-converting enzyme-1, neutral endopeptidase 24.11, and angiotensin-converting enzyme. PMID: 15838282
  14. Examine the effect of triple vasopeptidase inhibition of angiotensin-converting enzyme/neutral endopeptidase/endothelin-converting enzyme activities on the hemodynamic profile of spontaneously hypertensive rats. PMID: 15838331
  15. neutral endopeptidase activity is decreased by ascorbic acid in cultured osteoblastic cells PMID: 15908023
  16. The promoter of NEP is frequently methylated in hepatocellular carcinoma in contrast to its unmethylated status in normal liver. PMID: 16827801
  17. These results demonstrate that expression of neprilysin and aminopeptidase N can be regulated when peptide neurons are activated and, that regulation is enzyme-, region-, and stage-specific. PMID: 17952634
  18. NEP inhibition potentiates substance P-mediated neutrophil oxygen radical production and may promote other inflammatory activities during Mg-deficiency PMID: 18607539
  19. chromatin immunoprecipitation analysis also reveals amyloid precursor protein binding to the NEP promoter in rat primary neurons but not in HUVEC cells PMID: 19057576
  20. results show that acute immobilization stress affects differentially enkephalinase and oxytocinase activities depending on the fraction and brain region analyzed PMID: 19468242

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Subcellular Location
Cell membrane; Single-pass type II membrane protein.
Protein Families
Peptidase M13 family
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