Product Details

Description

This Rat MME ELISA Kit was designed for the quantitative measurement of Rat MME protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 47 pg/mL-3000 pg/mL and the sensitivity is 11.75 pg/mL.

Target Name

membrane metallo-endopeptidase

Alternative Names

MmeNeprilysin ELISA Kit; EC 3.4.24.11 ELISA Kit; Atriopeptidase ELISA Kit; Enkephalinase ELISA Kit; Neutral endopeptidase 24.11 ELISA Kit; NEP ELISA Kit; Neutral endopeptidase ELISA Kit; Skin fibroblast elastase ELISA Kit; SFE ELISA Kit; CD antigen CD10 ELISA Kit

Abbreviation

MME

Uniprot No.

P07861

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

47 pg/mL-3000 pg/mL

Sensitivity

11.75 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat MME in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	89-96
1:2	Average %	96
	Range %	91-103
1:4	Average %	98
	Range %	93-101
1:8	Average %	95
	Range %	90-99

Recovery

The recovery of rat MME spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-96
EDTA plasma (n=4)	98	93-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
3000	2.256	2.345	2.301	2.104
1500	1.634	1.536	1.585	1.388
750	1.128	1.214	1.171	0.974
375	0.774	0.787	0.781	0.584
187.5	0.479	0.482	0.481	0.284
94	0.357	0.365	0.361	0.164
47	0.268	0.269	0.269	0.072
0	0.195	0.199	0.197

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Target Background

Function (From Uniprot)	Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of the atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
Gene References into Functions	hypoxia increased the expression of platelet-derived growth factor (PDGF) and transforming growth factor-beta1 (TGF-beta1) and decreased the expression of neprilysin (NEP), which contributed to the hypoxia-induced Endothelial-to-mesenchymal transition of pulmonary artery endothelial cells. PMID: 27373199 The data obtained suggest that the increase of caspase-3 enzyme activity could affect NEP expression via proteolytic degradation of its transcription factor AICD PMID: 26983277 the increased expression of NPR-C (r=-0.943 and -0.837 for mRNA and protein respectively, p<0.05) and NEP (r=-0.687 and -0.823 for mRNA and protein respectively, p<0.05) were accompanied by a signi fi cant decline in CNP. PMID: 24189506 Data suggest that the brain expression of neprilysin may promote amyloid beta-protein (Abeta)deposition in patients with Alzheimer's disease (AD). PMID: 24508800 These results suggested that ketamine suppresses the Abeta degradation of NEP by reducing p38 MAPK-mediated pathway activity. PMID: 23624023 Naoerkang can increase the expression of NEP in hippocampal tissues of AD rats so as to reduce the quantity of AAbeta(1-42). PMID: 20141738 The three Abeta-degrading enzymes were damaged to different extents in the brain of diabetic rats, and impairment of ECE-1 and IDE partly contributed to the elevated Abeta(1-40) levels in brain of diabetic rats. PMID: 20414044 Androgen regulation of amyloid beta (Abeta)protein involves an androgen receptor-dependent mechanism requiring up-regulation of Abeta catabolizing enzyme neprilysin. PMID: 18346198 Ovariectomy increases BACE1 expression and decreases neprilysin expression in hippocampus. PMID: 20137687 Intracardiac expression of neutral endopeptidase PMID: 12030369 in spontaneously hypertensive rats, the local concentration and action of adrenomedullin in the tissues may be differentially regulated by NEP. PMID: 15005274 Neutral endopeptidase inactivity induces an exaggerated neurogenic inflammation mediated by neurokinin-1 receptor, which may lead to edema in pulmonary alveoli. PMID: 15194566 Report that CGS 35601 and its orally active prodrug CGS 37808 are triple inhibitors of endothelin-converting enzyme-1, neutral endopeptidase 24.11, and angiotensin-converting enzyme. PMID: 15838282 Examine the effect of triple vasopeptidase inhibition of angiotensin-converting enzyme/neutral endopeptidase/endothelin-converting enzyme activities on the hemodynamic profile of spontaneously hypertensive rats. PMID: 15838331 neutral endopeptidase activity is decreased by ascorbic acid in cultured osteoblastic cells PMID: 15908023 The promoter of NEP is frequently methylated in hepatocellular carcinoma in contrast to its unmethylated status in normal liver. PMID: 16827801 These results demonstrate that expression of neprilysin and aminopeptidase N can be regulated when peptide neurons are activated and, that regulation is enzyme-, region-, and stage-specific. PMID: 17952634 NEP inhibition potentiates substance P-mediated neutrophil oxygen radical production and may promote other inflammatory activities during Mg-deficiency PMID: 18607539 chromatin immunoprecipitation analysis also reveals amyloid precursor protein binding to the NEP promoter in rat primary neurons but not in HUVEC cells PMID: 19057576 results show that acute immobilization stress affects differentially enkephalinase and oxytocinase activities depending on the fraction and brain region analyzed PMID: 19468242 Show More Hide All
Subcellular Location	Cell membrane; Single-pass type II membrane protein.
Protein Families	Peptidase M13 family
Database Links	KEGG: rno:24590 STRING: 10116.ENSRNOP00000044578 UniGene: Rn.33598

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Check Code：

Code	CSB-EL014653RA
Size	96T,5×96T,10×96T
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Rat Neprilysin(MME) ELISA kit

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