Rat Neprilysin(MME) ELISA kit

Code CSB-EL014653RA
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This Rat MME ELISA Kit was designed for the quantitative measurement of Rat MME protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 47 pg/mL-3000 pg/mL and the sensitivity is 11.75 pg/mL.

Target Name membrane metallo-endopeptidase
Alternative Names
MmeNeprilysin ELISA Kit; EC ELISA Kit; Atriopeptidase ELISA Kit; Enkephalinase ELISA Kit; Neutral endopeptidase 24.11 ELISA Kit; NEP ELISA Kit; Neutral endopeptidase ELISA Kit; Skin fibroblast elastase ELISA Kit; SFE ELISA Kit; CD antigen CD10 ELISA Kit
Abbreviation MME
Uniprot No. P07861
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 47 pg/mL-3000 pg/mL
Sensitivity 11.75 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
To assess the linearity of the assay, samples were spiked with high concentrations of rat MME in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 92    
Range % 89-96    
1:2 Average % 96    
Range % 91-103    
1:4 Average % 98    
Range % 93-101    
1:8 Average % 95    
Range % 90-99    
The recovery of rat MME spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
Sample Type Average % Recovery Range    
Serum (n=5) 92 89-96    
EDTA plasma (n=4) 98 93-103    
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
pg/ml OD1 OD2 Average Corrected    
3000 2.256 2.345 2.301 2.104    
1500 1.634 1.536 1.585 1.388    
750 1.128 1.214 1.171 0.974    
375 0.774 0.787 0.781 0.584    
187.5 0.479 0.482 0.481 0.284    
94 0.357 0.365 0.361 0.164    
47 0.268 0.269 0.269 0.072    
0 0.195 0.199 0.197      
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

(From Uniprot)
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of the atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
Gene References into Functions
  1. hypoxia increased the expression of platelet-derived growth factor (PDGF) and transforming growth factor-beta1 (TGF-beta1) and decreased the expression of neprilysin (NEP), which contributed to the hypoxia-induced Endothelial-to-mesenchymal transition of pulmonary artery endothelial cells. PMID: 27373199
  2. The data obtained suggest that the increase of caspase-3 enzyme activity could affect NEP expression via proteolytic degradation of its transcription factor AICD PMID: 26983277
  3. the increased expression of NPR-C (r=-0.943 and -0.837 for mRNA and protein respectively, p<0.05) and NEP (r=-0.687 and -0.823 for mRNA and protein respectively, p<0.05) were accompanied by a signi fi cant decline in CNP. PMID: 24189506
  4. Data suggest that the brain expression of neprilysin may promote amyloid beta-protein (Abeta)deposition in patients with Alzheimer's disease (AD). PMID: 24508800
  5. These results suggested that ketamine suppresses the Abeta degradation of NEP by reducing p38 MAPK-mediated pathway activity. PMID: 23624023
  6. Naoerkang can increase the expression of NEP in hippocampal tissues of AD rats so as to reduce the quantity of AAbeta(1-42). PMID: 20141738
  7. The three Abeta-degrading enzymes were damaged to different extents in the brain of diabetic rats, and impairment of ECE-1 and IDE partly contributed to the elevated Abeta(1-40) levels in brain of diabetic rats. PMID: 20414044
  8. Androgen regulation of amyloid beta (Abeta)protein involves an androgen receptor-dependent mechanism requiring up-regulation of Abeta catabolizing enzyme neprilysin. PMID: 18346198
  9. Ovariectomy increases BACE1 expression and decreases neprilysin expression in hippocampus. PMID: 20137687
  10. Intracardiac expression of neutral endopeptidase PMID: 12030369
  11. in spontaneously hypertensive rats, the local concentration and action of adrenomedullin in the tissues may be differentially regulated by NEP. PMID: 15005274
  12. Neutral endopeptidase inactivity induces an exaggerated neurogenic inflammation mediated by neurokinin-1 receptor, which may lead to edema in pulmonary alveoli. PMID: 15194566
  13. Report that CGS 35601 and its orally active prodrug CGS 37808 are triple inhibitors of endothelin-converting enzyme-1, neutral endopeptidase 24.11, and angiotensin-converting enzyme. PMID: 15838282
  14. Examine the effect of triple vasopeptidase inhibition of angiotensin-converting enzyme/neutral endopeptidase/endothelin-converting enzyme activities on the hemodynamic profile of spontaneously hypertensive rats. PMID: 15838331
  15. neutral endopeptidase activity is decreased by ascorbic acid in cultured osteoblastic cells PMID: 15908023
  16. The promoter of NEP is frequently methylated in hepatocellular carcinoma in contrast to its unmethylated status in normal liver. PMID: 16827801
  17. These results demonstrate that expression of neprilysin and aminopeptidase N can be regulated when peptide neurons are activated and, that regulation is enzyme-, region-, and stage-specific. PMID: 17952634
  18. NEP inhibition potentiates substance P-mediated neutrophil oxygen radical production and may promote other inflammatory activities during Mg-deficiency PMID: 18607539
  19. chromatin immunoprecipitation analysis also reveals amyloid precursor protein binding to the NEP promoter in rat primary neurons but not in HUVEC cells PMID: 19057576
  20. results show that acute immobilization stress affects differentially enkephalinase and oxytocinase activities depending on the fraction and brain region analyzed PMID: 19468242

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Subcellular Location Cell membrane; Single-pass type II membrane protein.
Protein Families Peptidase M13 family
Database Links

KEGG: rno:24590

STRING: 10116.ENSRNOP00000044578

UniGene: Rn.33598

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