Recombinant Rat Neuroendocrine convertase 1 (Pcsk1)

Code CSB-YP017640RA
MSDS
Size $368
Order now
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
Pcsk1
Uniprot No.
Research Area
Signal Transduction
Alternative Names
Pcsk1; Bdp; Nec-1; Nec1Neuroendocrine convertase 1; NEC 1; EC 3.4.21.93; Prohormone convertase 1; Proprotein convertase 1; PC1
Species
Rattus norvegicus (Rat)
Source
Yeast
Expression Region
111-752aa
Target Protein Sequence
SVPRDSALNLFNDPMWNQQWYLQDTRMTASLPKLDLHVIPVWQKGITGKGVVITVLDDGLEWNHTDIYANYDPEASYDFNDNDHDPFPRYDPTNENKHGTRCAGEIAMQANNHKCGVGVAYNSKVGGIRMLDGIVTDAIEASSIGFNPGHVDIYSASWGPNDDGKTVEGPGRLAQKAFEYGVKQGRQGKGSIFVWASGNGGRQGDNCDCDGYTDSIYTISISSASQQGLSPWYAEKCSSTLATSYSSGDYTDQRITSADLHNDCTETHTGTSASAPLAAGIFALALEANPNLTWRDMQHLVVWTSEYDPLANNPGWKKNGAGLMVNSRFGFGLLNAKALVDLADPRTWRNVPEKKECIIKDNNFEPRALKANGEVIVEIPTRACEGQENAINSLEHVQFEATIEYSRRGDLHVTLTSAAGTSTVLLAERERDTSPNGFKNWDFMSVHTWGENPVGTWTLKVTDMSGRMQNEGRIVNWKLILHGTSSQPEHMKQPRVYTSYNTVQNDRRGVEKMVNVVEEKPTQNSLNGNLLVPKNSSSSSVEDRRDEQVQGAPSKAMLRLLQSAFSKNTPSKQSSKIPSAKLSVPYEGLYEALEKLNKPSQLEDSEDSLYSDYVDVFYNTKPYKHRDDRLLQALMDILNEKN
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
73.2kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin, insulin and AGRP.
Gene References into Functions
  1. macrophages from PC1/3 KO mice and rat PC1/3-KD NR8383 macrophages secreted more pro-inflammatory cytokines such as TNF-alpha, IL6, IL1alpha and CXCL2. PMID: 26778167
  2. PC1/3 as an important regulator of vesicle trafficking and secretion in macrophages. PMID: 23637853
  3. Chronic ethanol exposure decreases prohormone convertase (pc1/3) immunoreactivity in brain. PMID: 23050949
  4. catalytic and P domains contribute to the folding and thermodynamic stability of the convertases through reciprocal hydrophobic interactions (prohormone convertase 3 [pc3/pc1]) PMID: 12721373
  5. The prohormone convertase (PC) enzyme PC1 together with PC2 and PC5 may represent a redundant system to ensure production of biologically active cholecystokinin in rat brain. PMID: 14608596
  6. proGHRH is initially cleave by furin at preproGHRH29-30, followed by a second cleavage at preproGHRH74 primarily by PC1 to generate GHRH and GHRH-RP peptides, respectively. PMID: 15146101
  7. Leptin regulates hypothalamic PC1 expression and requires prohormone processing into mature peptides. PMID: 15286802
  8. Prohormone-convertase 1 processing enhances post-Golgi sorting of prothyrotropin-releasing hormone-derived peptides PMID: 16204236
  9. the prohormone convertase cleavage site within the alpha-helix mediates sorting of the neuronal and endocrine polypeptide VGF into the regulated secretory pathway PMID: 16221685
  10. The observed decrease in CCK seen with these RNAi treatments further supports a role for PC1 in CCK processing in these cells PMID: 16274843
  11. Agouti-related protein is posttranslationally cleaved by proprotein convertase 1 to generate agouti-related protein (AGRP)83-132. PMID: 16384863
  12. These data indicate that the transmembrane domain of PC3 plays a key role in sorting the enzyme to the regulated secretory pathway. PMID: 17240044
  13. Ontogeny of ghrelin, obestatin, preproghrelin, and prohormone convertases in rat pancreas and stomach. PMID: 18784614

Show More

Hide All

Subcellular Location
Cytoplasmic vesicle, secretory vesicle. Note=Localized in the secretion granules.
Protein Families
Peptidase S8 family, Furin subfamily
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
Place an order now

I. Product details

*
*
*
*

II. Contact details

*
*

III. Ship To

*
*
*
*
*
*
*

IV. Bill To

*
*
*
*
*
*
*
*