Recombinant Rat Nuclear factor erythroid 2-related factor 2(Nfe2l2)

Code CSB-YP015752RA
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Source Yeast
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Code CSB-EP015752RA
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Source E.coli
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Code CSB-EP015752RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015752RA
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Source Baculovirus
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Code CSB-MP015752RA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Nfe2l2
Uniprot No. O54968
Alternative Names Nfe2l2; Nrf2; Nuclear factor erythroid 2-related factor 2; NF-E2-related factor 2; NFE2-related factor 2; Nuclear factor; erythroid derived 2; like 2
Species Rattus norvegicus (Rat)
Expression Region 1-604
Target Protein Sequence MMDLELPPPG LQSQQDMDLI DILWRQDIDL GVSREVFDFS QRQKDYELEK QKKLEKERQE QLQKEQEKAF FAQLQLDEET GEFLPIQPAQ HIQTDTSGSV SYSQVAHIPK QDALYFEDCM QLLAETFPFV DDHEVSSPTF QSLALDIPSH VESSVFTTPD QAQSLDSSLE TAMTDLSSIQ QDMEQVWQEL FSIPELQCLN TENKQQAETT TVPSPEATLT EMDSNYHFYS SIPSLEKEVD SCSPHFLHGF EDSFSSILST DDASQLNSLD SNPTLNTDFG DEFYSAFLAE PSGGGSMPSS AAISQSLSEL LGGPIEGCDL SLCKAFNQKH TEGTVEFNDS DSGISLNTSP SRASPEHSVE SSIYGDPPPG FSDSEMEELD SAPGSVKQNG PKAQPTHSSG DTVQPLSPAQ GHSAAVHESQ CENTTKKEVP VSPGHQKVPF TKDKHSSRLE AHLTRDELRA KALHIPFPVE KIINLPVDDF NEMMSKEQFN EAQLALIRDI RRRGKNKVAA QNCRKRKLEN IVELEQDLGH LKDEREKLLR EKGENDRNLH LLKRKLSTLY LEVFSMLRDE DGKPYSPSEY SLQQTRDGNV FLVPKSKKPD TKKN
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Transcription factor that plays a key role in the response to oxidative stress: binds to antioxidant response (ARE) elements present in the promoter region of many cytoprotective genes, such as phase 2 detoxifying enzymes, and promotes their expression, thereby neutralizing reactive electrophiles. In normal conditions, ubiquitinated and degraded in the cytoplasm by the BCR(KEAP1) complex. In response to oxidative stress, electrophile metabolites inhibit activity of the BCR(KEAP1) complex, promoting nuclear accumulation of NFE2L2/NRF2, heterodimerization with one of the small Maf proteins and binding to ARE elements of cytoprotective target genes. The NFE2L2/NRF2 pathway is also activated in response to selective autophagy: autophagy promotes interaction between KEAP1 and SQSTM1/p62 and subsequent inactivation of the BCR(KEAP1) complex, leading to NFE2L2/NRF2 nuclear accumulation and expression of cytoprotective genes. May also be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Plays also an important role in the regulation of the innate immune response. It is a critical regulator of the innate immune response and survival during sepsis by maintaining redox homeostasis and restraint of the dysregulation of proinflammatory signaling pathways like MyD88-dependent and -independent and TNF-alpha signaling. Suppresses macrophage inflammatory response by blocking proinflammatory cytokine transcription and the induction of IL6. Binds to the proximity of proinflammatory genes in macrophages and inhibits RNA Pol II recruitment. The inhibition is independent of the Nrf2-binding motif and reactive oxygen species level. Represses antiviral cytosolic DNA sensing by suppressing the expression of the adapter protein STING1 and decreasing responsiveness to STING1 agonists while increasing susceptibility to infection with DNA viruses.
Gene References into Functions
  1. SETD7 gene silencing attenuates hypoxia/reoxygenation-induced injury of cardiomyocytes via the down-regulation of Keap1 and promotion of the Nrf2-mediated anti-oxidation signaling pathway. PMID: 30119254
  2. The results also demonstrated a temporal relationship between Nrf2 and system xc(-) which could be implicated in the initiation of oxidative stress in retina in diabetes. PMID: 29488137
  3. AS-IV evoked Akt phosphorylation, and subsequent induced phosphorylation of glycogen synthase kinase-3beta (GSK-3beta) at Ser9 (that is, inactivation), which stimulated Nrf2-mediated antioxidant response element (ARE)-containing activation. PMID: 28315454
  4. cigarette smoke exposure-dependent alterations in lung morphology and parameters and cardiac disorder in COPD are concurrent with oxidative stress, particularly, in Nrf2 pathway (both upstream and downstream genes) PMID: 29631592
  5. the results of the present study demonstrated that the protective role of hyperoxia liquid may regulate oxidative stress in a cardiopulmonary bypassinducedrat model through the Nrf2antioxidant response element signaling pathway. PMID: 29901143
  6. Downregulation of NRF2 promotes inflammatory response in lung tissues of sepsis-associated acute respiratory distress syndrome. PMID: 30121199
  7. Hypothermic machine perfusion attenuates ischemia/reperfusion injury against rat livers donated after cardiac death by activating the Keap1/Nrf2ARE signaling pathway. PMID: 29845199
  8. Our data provide novel insights for investigating DOX-induced heart injury. In addition, miR-140-5p/ Nrf2 and miR-140-5p/Sirt2 may be the new targets to treat DOX-induced cardiotoxicity. PMID: 29304479
  9. miR-503 is involved in the protective effect of CPDT in diabetic cardiomyopathy via Nrf2/ARE signaling pathway; miR-503 and Nrf2 may be a promising therapeutic target for the management of diabetic cardiomyopathy. PMID: 29404371
  10. n-propargyl caffeamide (PACA) attenuated LPS-induced NF-kB activation while activated Nrf2/HO-1 pathway. HO-1 inhibitor SnPP attenuated the effects of PACA on iNOS expression in LPS-challenged macrophages, possibly by regulating the cross-talk between HO-1 and NF-kB pathways. PMID: 29996121
  11. All the findings indicated that Proanthocyanidinscould protect against the oxidative damage induced by H2O2 in tendon-derived stem cells , and the cytoprotective effects might be due to the ability of PCs to activate the expressions of GCLM, HO-1, and NQO-1 via upregulating Nrf-2 signaling pathway PMID: 29201913
  12. The high survival ratio of Schwann cells in a diabetic peripheral neuropathy rat model indicates that overexpression of Nrf2 restores nerve injury. PMID: 29719281
  13. Results show that BDNF constitutively controlled the nuclear translocation of Nrf2. Low BDNF levels in vulnerable animals prevented Nrf2 translocation and consequently prevented the activation of detoxifying/antioxidant enzymes. Activating Nrf2 translocation restored redox homeostasis and reversed vulnerability to depression. PMID: 27646262
  14. myricetin treatment significantly increased the activity of Nrf2/HO-1 pathway, strengthening antioxidative stress capacity evidenced by reversed activities of GPx and SOD, and decreased MDA production. PMID: 29147465
  15. findings suggested that L-arginine/NO reduces renal dysfunction associated with I/R of the kidney and may act as a trigger to regulate the NF-kappaB, HSP70 and Nrf2/HO-1 signaling cascades. PMID: 28854440
  16. Data (including data from studies in transgenic rats and knockout mice) suggest that Nfe2l2 and Prdx6 are involved in biosynthesis of palmitic acid ester of 9-hydroxystearic acid in white adipose tissue (WAT); these proteins appear to be play roles in defense against oxidative stress in WAT. (Nfe2l2 = nuclear factor erythroid 2-like 2 nuclear factor; Prdx6 = peroxiredoxin-6) PMID: 29549163
  17. the AMPK-nuclear factor-erythroid 2 p45-related factor 2 (Nrf2) pathway as a mechanism whereby pomegranate extract reduces oxidative stress in the paraventricular nucleus to relieve hypertension. PMID: 27713551
  18. Sulforaphane protects against the development of doxorubicin-induced chronic heart failure and is associated with up-regulation of Nrf2 expression/transcription. PMID: 28636290
  19. Nrf2/HO-1 signaling pathway may be the primary target for protection from cisplatin-induced nephrotoxicity by SA. PMID: 28686978
  20. Endogenous NRF2 expression in primary rat neurons was stimulated by transfection with an ARE-mApple plasmid plus tBHQ. PMID: 28028237
  21. As melatonin effectively rescues hippocampal bioenergetics through depressing the oxidative stress by promoting Nrf2-ARE molecular machinery, this study thus highlights for the first time that clinical use of melatonin may serve as a therapeutic strategy to improve the cognitive function in unsuspecting victims suffered from gamma-hydroxybutyric acid intoxication injury. PMID: 28480587
  22. Pomegranate emulsion induced Nrf2 expression during 9,10-dimethyl-1,2-benzanthracene mammary tumorigenesis. PMID: 28452959
  23. This study points to the modulatory effect of C. molmol on glutamate-NO-cGMP and Nrf2/ARE/HO-1 pathways in hyperammonemia. Therefore, C. molmol might be a promising protective agent against hyperammonemia. PMID: 28744340
  24. NIC pretreatment effectively inhibit CME-induced myocardial apoptosis and improve cardiac function. The protective effects are mediated through the activation of the Nrf2/HO-1 signaling in cardiomyocytes. PMID: 29412163
  25. Myricetin treatment activates the Nrf2 pathway and leads to a significant accumulation of Nrf2 nuclear translocation. Myricetin attenuates brain injury and neurological deficits in a rat model of cerebral ischemia via improvement of mitochondrial function and activation of the Nrf2 pathway. PMID: 27171848
  26. Antifatigue Effect of Luteolin-6-C-Neohesperidoside on Oxidative Stress Injury Induced by Forced Swimming of Rats through Modulation of Nrf2/ARE Signaling Pathways. PMID: 28588747
  27. Nrf2 knockout rat is sensitive to development of aflatoxin B1 liver toxicity. PMID: 27071940
  28. Activation of Nrf2-ARE signaling pathway may prevent hyperphosphatemia-induced VC by inducing autophagy in VSMCs. PMID: 28513870
  29. The immunohistochemistry results showed that Nrf2 was absent in fibroblastic foci (FF) areas, while the SalB treatment could increase the expression of Nrf2 in lung tissues, especially in FF areas. PMID: 29108993
  30. Methane-rich saline treatment prevents ischemia reperfusion-induced spinal cord damage via antioxidant, anti-inflammatory and anti-apoptotic activities that involve the activation of Nrf2 signaling. PMID: 28007572
  31. Downregulation of histone acetylation can inhibit manganese chloride-induced Nrf2 nuclear translocation and further inhibits the Nrf2/HO-1 pathway I manganese neurotoxicity syndromes. PMID: 29054681
  32. Ptgr1 was overexpressed during the early phase in altered hepatocyte foci, and this high level of expression was maintained in persistent nodules until tumors developed. Ptgr1 expression was regulated by NRF2, which bound to an antioxidant response element at -653bp in the rat Ptgr1 gene. PMID: 27867096
  33. Hemoglobin-activated astrocytes acquired resistance to hemin-induced toxicity via Nrf2/HO-1 pathway. This phenomenon can be considered as the adaptive self-defense in the pathological process of intracerebral hemorrhage. PMID: 29074371
  34. The cytoprotective effect of MLN4924 was associated with its ability to diminish ROS production induced by H2O2 and the accumulation of Nrf2 protein levels in the cytoplasm and nucleus of the CGNs. PMID: 26966893
  35. We also found evidence that PB treatment reversed the suppression of Nrf2 nuclear translocation, and increased the expressions of NQO1 and HO-1 in the prostate glands. In conclusion, treatment with PB attenuates carrageenan-induced chronic nonbacterial prostatitis via anti-inflammatory and activation of the Nrf2 pathway. PMID: 28844677
  36. The expression of Nrf2/HO-1 increased in mesenteric arteries of portal hypertensive rats, which was related to oxidative stress. PMID: 28025064
  37. The protective effects of curcumin against cerebral I/R injury reflect anti-oxidation, anti-inflammation and anti-apoptotic activities, resulting in the elevation of Nrf2 and down-regulation of NF-kappaB. PMID: 26686249
  38. Exendin-4 ameliorates HG-induced cardiomyocyte apoptosis, and the mechanisms may involve anti-oxidative stress via the HO-1/Nrf-2 system, as well as intervention of the PI3K/AKT signaling pathway. PMID: 28464602
  39. this study shows that H2S may exert antioxidant effect on cardiac hypertrophy via PI3K/Akt-dependent activation of Nrf2 pathway PMID: 28423592
  40. IL-22 inhibits acetaldehyde-induced hepatic stellate cells activation and proliferation, which may be related to nuclear translocation of Nrf2 and increased activity of the antioxidant axis Nrf2-keap1-ARE. PMID: 28373766
  41. CAPE inhibits cell proliferation and up-regulates the antioxidant levels in HSC-T6 cells partly through the Nrf2-MAPKs signaling pathway PMID: 28275300
  42. Nrf2 activator, dh404, reduces oxidative stress in Muller cells to prevent ischemic retinopathy. PMID: 27005782
  43. Results suggest that sulforaphane has a therapeutic benefit in post-subarachnoid hemorrhage, and this may be due to elevated Nrf2-ARE pathway activity and inhibition of cerebral vascular proinflammatory cytokine expression PMID: 27693416
  44. Our results documented that BM-derived MSCs exerts considerable reversing effect of histopathologic and ultrastructural hypertensive nephropathy. Moreover, immunohistochemical results clearly pointed to relevant role of Nrf2 pathway in MSCs related renal therapeutic effects. PMID: 28256256
  45. The Exendin-4-evoked Nrf2 acetylation favored its nuclear retention. PMID: 27488664
  46. These results identify a novel regulatory role for Nrf2 in neural stem/progenitor cell function during aging. PMID: 27095375
  47. Plasmids (pDC315 or pDC315/Nrf2) were loaded onto nanomicrobubbles, and then injected through the tail vein with the assistance of MRI-guided focused ultrasound. MRI-guided focused ultrasound delivery of nanomicrobubbles increased gene transfection efficiency. Furthermore, Nrf2 gene transfection reduced reactive oxygen species levels, thereby protecting neurons in the target region. PMID: 27810365
  48. NRF-2, in addition to regulating the coupling between neuronal activity and energy metabolism, also regulates the expression of BDNF, which is intimately associated with energy-demanding neuronal activity. PMID: 27624937
  49. alpha-Boswellic acid decreases oxidative stress. Nrf2/HO-1 pathway might play a role in the gastroprotective action of alpha-BA in ethanol-induced gastric injury in rats. PMID: 26992040
  50. study shows that SIRT2 regulates nuclear Nrf2 levels by modulating Akt phosphorylation, thus modulating the levels of GCL and total glutathione. PMID: 27264719

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Subcellular Location Cytoplasm, cytosol. Nucleus.
Protein Families BZIP family, CNC subfamily
Database Links

KEGG: rno:83619

STRING: 10116.ENSRNOP00000002114

UniGene: Rn.10867

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