Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Uniprot No.

P12346

Research Area

Epigenetics and Nuclear Signaling

Alternative Names

Tf; Serotransferrin; Transferrin; Beta-1 metal-binding globulin; Liver regeneration-related protein LRRG03; Siderophilin

Species

Rattus norvegicus (Rat)

Source

E.coli

Expression Region

20-698aa

Target Protein Sequence

VPDKTVKWCAVSEHENTKCISFRDHMKTVLPADGPRLACVKKTSYQDCIKAISGGEADAITLDGGWVYDAGLTPNNLKPVAAEFYGSLEHPQTHYLAVAVVKKGTDFQLNQLQGKKSCHTGLGRSAGWIIPIGLLFCNLPEPRKPLEKAVASFFSGSCVPCADPVAFPQLCQLCPGCGCSPTQPFFGYVGAFKCLRDGGGDVAFVKHTTIFEVLPQKADRDQYELLCLDNTRKPVDQYEDCYLARIPSHAVVARNGDGKEDLIWEILKVAQEHFGKGKSKDFQLFGSPLGKDLLFKDSAFGLLRVPPRMDYRLYLGHSYVTAIRNQREGVCPEGSIDSAPVKWCALSHQERAKCDEWSVSSNGQIECESAESTEDCIDKIVNGEADAMSLDGGHAYIAGQCGLVPVMAENYDISSCTNPQSDVFPKGYYAVAVVKASDSSINWNNLKGKKSCHTGVDRTAGWNIPMGLLFSRINHCKFDEFFSQGCAPGYKKNSTLCDLCIGPAKCAPNNREGYNGYTGAFQCLVEKGDVAFVKHQTVLENTNGKNTAAWAKDLKQEDFQLLCPDGTKKPVTEFATCHLAQAPNHVVVSRKEKAARVSTVLTAQKDLFWKGDKDCTGNFCLFRSSTKDLLFRDDTKCLTKLPEGTTYEEYLGAEYLQAVGNIRKCSTSRLLEACTFHKS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

89.9 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-KSI-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Tris-based buffer,50% glycerol

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The generation of the recombinant Rat Serotransferrin protein typically includes the following steps: synthesizing the recombinant plasmid containing the gene encoding the Rat Serotransferrin protein (20-698aa), transforming the recombinant plasmid into e.coli cells, selecting the positive e.coli cells, and culturing the positive e.coli cells for protein expression. The protein is equipped with a N-terminal 6xHis-KSI tag. After expression, affinity purification is employed to isolate and purify the recombinant Serotransferrin protein from the cell lysate. Denaturing SDS-PAGE is utilized to resolve the resulting recombinant Rat Serotransferrin protein, revealing a purity level exceeding 85%.

Target Background

Function

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.

Gene References into Functions

our data establish that blood TSAT exerts a critical role in experimental stroke-induced brain damage. In addition, our findings suggest that the protective effect of iron-free transferrin (apotransferrin, ATf) at the neuronal level resides in preventing NMDA-induced HTf uptake and ROS production, which in turn reduces neuronal damage PMID: 29248829
In brain interstitial fluid, transferrin-bound iron and non-transferrin-bound iron (NTBI) exist as potential iron sources PMID: 25649872
Transferrin and thyroid hormone converge to regulate oligodendrocyte maturation and myelinogenesis. PMID: 25595122
This evidence supports a key role of Tf on the generation of oligodendrocytes (OLs) from Neural stem and progenitor cells (NSC/NPCs)and highlights its potential in demyelinating disorder treatment. PMID: 22479482
Transferrin is involved in the uptake of the radiopharmaceutical gallium-67 (67Ga) by regenerating liver tissue following partial hepatectomy but is not involved in the entry of 67Ga into hepatocytes. PMID: 11767098
data show that brain cells have the capacity to maintain minimum iron levels during iron deficiency which may be associated with increased iron-Tf uptake, stabilization of TfR mRNA, or increased Tf mRNA translation efficiency in specific cell types PMID: 12608731
effects of apotransferrin occur early in development during a narrow, transient "temporal window" within which oligodendroglia are sensitive to its action. PMID: 12730961
first report describing presence of serotransferrin precursor protein homolog belonging to transferrin family and sharing epitopes with melanotransferrin in adrenal, its induction by ACTH, and sensitivity to ginkgolide B (adrenotransferrin). PMID: 14701678
compared to transferrin, soluble melanotransferrin plays little or no role in Fe supply to the brain and erythropoietic tissue PMID: 15469901
in addition to hemoglobin-bound iron, Tf-bound iron may contribute to intracerebral hemorrhage-induced brain injury and thrombin may contribute to the latter by facilitating cellular iron uptake PMID: 15637325
Our studies suggest that AEC I is not just a simple barrier for gas exchange, but a functional cell that protects alveolar epithelium from injury. PMID: 16497717
Plays role in maturation of Schwann cells(SC) in physiological conditions as well as in role in facilitating clearance of myelin debris and subsequent nerve regeneration and/or in SC phenotypic change, which led to survival after peripheral nerve injury. PMID: 17628542
Apotransferrin added to oligodendroglial cell cultures can correct the effects of iron deficiency. PMID: 18459135
serotransferrin levels were increased in diestrous as compared with proestrous, estrous, metestrous PMID: 18511206
we have discovered a novel mitochondrial iron transport system that goes awry in Parkinson's disease, and which may provide a new target for therapeutic intervention. PMID: 19250966
synthesis and secretion of transferrin in rat parotid acinar cells PMID: 19464997

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Subcellular Location

Secreted.

Protein Families

Transferrin family

Tissue Specificity

Expressed by the liver and secreted in plasma.

Database Links

KEGG: rno:24825

STRING: 10116.ENSRNOP00000012725

UniGene: Rn.91296

Code	CSB-EP023412RA
Abbreviation	Recombinant Rat Serotransferrin protein
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Rat Serotransferrin (Tf)

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