Product Details

Purity

>85% (SDS-PAGE)

Target Names

Syk

Uniprot No.

Q64725

Alternative Names

SykTyrosine-protein kinase SYK; EC 2.7.10.2; Spleen tyrosine kinase; p72Syk

Species

Rattus norvegicus (Rat)

Expression Region

1-629

Target Protein Sequence

MAGNAVDNAN HLTYFFGNIT REEAEDYLVQ GGMTDGLYLL RQSRNYLGGF ALSVAHNRKA HHYTIERELN GTYAISGGRA HASPADLCHY HSQEPEGLVC LLKKPFNRPP GVQPKTGPFE DLKENLIREY VKQTWNLQGQ ALEQAIISQK PQLEKLIATT AHEKMPWFHG NISRDESEQT VLIGSKTNGK FLIRARDNNG SFALCLLHEG KVLHYRIDRD KTGKLSIPEG KKFDTLWQLV EHYSYKPDGL LRVLTVPCQK IGVQMGHPGS SNAHPVTWSP GGIISRIKSY SFPKPGHKKP PPPQGSRPES TVSFNPYEPT GGAWGPDRGL QREALPMDTE VYESPYADPE EIRPKEVYLD RKLLTLEDNE LGSGNFGTVK KGYYQMKKVV KTVAVKILKN EANDPALKDE LLAEANVMQQ LDNPYIVRMI GICEAESWML VMEMAAWGPL NKYLQQNRHI KDKNIIELVH QVSMGMKYLE ESNFVHRDLA ARNVLLVTQH YAKISDFGLS KALRADENYY KAQTHGKWPV KWYAPECINY FKFSSKSDVW SFGVLMWEAF SYGQKPYRGM KGSEVTAMLE KGERMGCPPG CPREMYDLMF LCWTYDVENR PGFAAVELRL RNYYYDVVN

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Non-receptor tyrosine kinase which mediates signal transduction downstream of a variety of transmembrane receptors including classical immunoreceptors like the B-cell receptor (BCR). Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development. Assembles into signaling complexes with activated receptors at the plasma membrane via interaction between its SH2 domains and the receptor tyrosine-phosphorylated ITAM domains. The association with the receptor can also be indirect and mediated by adapter proteins containing ITAM or partial hemITAM domains. The phosphorylation of the ITAM domains is generally mediated by SRC subfamily kinases upon engagement of the receptor. More rarely signal transduction via SYK could be ITAM-independent. Direct downstream effectors phosphorylated by SYK include VAV1, PLCG1, PI-3-kinase, LCP2 and BLNK. Initially identified as essential in B-cell receptor (BCR) signaling, it is necessary for the maturation of B-cells most probably at the pro-B to pre-B transition. Activated upon BCR engagement, it phosphorylates and activates BLNK an adapter linking the activated BCR to downstream signaling adapters and effectors. It also phosphorylates and activates PLCG1 and the PKC signaling pathway. It also phosphorylates BTK and regulates its activity in B-cell antigen receptor (BCR)-coupled signaling. In addition to its function downstream of BCR plays also a role in T-cell receptor signaling. Plays also a crucial role in the innate immune response to fungal, bacterial and viral pathogens. It is for instance activated by the membrane lectin CLEC7A. Upon stimulation by fungal proteins, CLEC7A together with SYK activates immune cells inducing the production of ROS. Also activates the inflammasome and NF-kappa-B-mediated transcription of chemokines and cytokines in presence of pathogens. Regulates neutrophil degranulation and phagocytosis through activation of the MAPK signaling cascade. Required for the stimulation of neutrophil phagocytosis by IL15. Also mediates the activation of dendritic cells by cell necrosis stimuli. Also involved in mast cells activation. Involved in interleukin-3/IL3-mediated signaling pathway in basophils. Also functions downstream of receptors mediating cell adhesion. Relays for instance, integrin-mediated neutrophils and macrophages activation and P-selectin receptor/SELPG-mediated recruitment of leukocytes to inflammatory loci. Plays also a role in non-immune processes. It is for instance involved in vascular development where it may regulate blood and lymphatic vascular separation. It is also required for osteoclast development and function. Functions in the activation of platelets by collagen, mediating PLCG2 phosphorylation and activation. May be coupled to the collagen receptor by the ITAM domain-containing FCER1G. Also activated by the membrane lectin CLEC1B that is required for activation of platelets by PDPN/podoplanin. Involved in platelet adhesion being activated by ITGB3 engaged by fibrinogen. Together with CEACAM20, enhances production of the cytokine CXCL8/IL-8 via the NFKB pathway and may thus have a role in the intestinal immune response.

Gene References into Functions

mast cells contribute to Mincle-mediated immunity through Syk activation triggered by association with the FcepsilonRIbetagamma complex. PMID: 28393919
present results determined that high glucoseinduced activation of the NLRP3 inflammasome is mediated by Syk/JNK activation, which subsequently increased the protein expression level of IL1beta and mature IL1beta. The present study identified that the Syk/JNK/NLRP3 signaling pathway may serve a vital role in the pathogenesis ofdiabetic nephropathy. PMID: 29901140
Cryptotanshinone inhibits IgEmediated degranulation through inhibition of spleen tyrosine kinase and tyrosineprotein kinase phosphorylation in mast cells. PMID: 29845271
Brain Syk expression is upregulated after subarachnoid hemorrhage. PMID: 26138128
The results obtained in simulated microgravity show that ROS production in macrophages is a highly gravisensitive process, caused by a diminished Syk phosphorylation. PMID: 25644261
Identify role for Syk in acute renal allograft rejection which is independent of T-cell activation. PMID: 25529862
Syk is critical for Dectin-1-mediated activation of mast cells, although the signaling differs from that triggered by FcRI activation PMID: 25246527
expressed and activated in antiglomerular basement membrane disease PMID: 24700868
Syk is present in neurons of different structures including the cerebellum, the hippocampus, the visual system and the olfactory system; Syk connects immunoreceptors to downstream adaptor/effector molecules in these neurons. PMID: 21354221
Syk signalling is required for JNK and p38 MAPK signalling and acute neutrophil-dependent glomerular injury in rat nephrotoxic serum nephritis. PMID: 21894146
ANG II stimulates angiogenesis via transactivation of the EGF receptor, which promotes the phosphorylation of Flt-1 and activation of Syk independent of VEGF expression. PMID: 21642504
Data suggest that Syk plays an important role in vascular remodeling through the modulation of proliferation and phenotypes of VSMCs. PMID: 21189061
Syk plays an important role in vascular remodeling by changing the phenotypes and cytoskeleton of vascular smooth muscle cells stimulated by PDGF-BB. PMID: 21055270
Syk may promote pulmonary vascular smooth muscle cells (PVSMCs) proliferation induced by PDGF-BB. PMID: 21083985
Acdtivation is required for c-Cbl-mediated ubiquitination of Fcepsilon RI and Syk in RBL cells PMID: 12145291
Data show that tyr342, but not tyr346, is critical for regulating Syk in mast cells. PMID: 12417718
Syk signaling is mediated in an ITAM-based fashion by tamalin PMID: 15173175
Alpha-melanocyte stimulating hormone protects against H2O2-induced inhibition of wound healing via a Syk kinase- and NF-kappabeta-dependent mechanism PMID: 15489638
Phosphorylation of Tyr218 of the FcepsilonRI-beta seems to be most important for Syk binding. Recruitment of Syk and other signaling proteins to the beta-subunit might be important for its amplifier role. PMID: 17365510
Our data support the hypothesis that, in macrophages, Syk is involved in LPS-induced intracellular signaling pathways leading to the release of pro-inflammatory mediators PMID: 17621553
3D structure of Syk kinase. PMID: 18021750
reveal a positive feedback step in mast cell activation where receptor-triggered Syk activation and subsequent Ca2+ release opens Ca2+ release-activated Ca2+ channels PMID: 18806259
Gene transfer of redox factor-1 inhibits neointimal formation: involvement of platelet-derived growth factor-beta receptor signaling via the inhibition of the reactive oxygen species-mediated Syk pathway. PMID: 19038866
conformational changes in an activated form of Syk induced by auto-phosphorylation; results suggest regulation of the activation of Syk might be modulated by subtle changes in the positioning of the regulatory domains rather than a full opening mechanism PMID: 19409513
Syk couples Ca(2+) microdomains to the activation of two spatially and temporally distinct cellular responses, revealing the versatility of local Ca(2+) signals in driving cell activation PMID: 19584058

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Subcellular Location

Cell membrane. Cytoplasm, cytosol.

Protein Families

Protein kinase superfamily, Tyr protein kinase family, SYK/ZAP-70 subfamily

Database Links

KEGG: rno:25155

STRING: 10116.ENSRNOP00000016942

UniGene: Rn.87407

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Recombinant Rat Tyrosine-protein kinase SYK (Syk)

Product Details

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Target Background

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