Recombinant Saccharomyces cerevisiae GTP-binding nuclear protein GSP1/CNR1 (GSP1)

Code CSB-BP333740SVG
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Source Baculovirus
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Code CSB-EP333740SVG-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-MP333740SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GSP1
Uniprot No.
Alternative Names
GSP1; CNR1; CST17; YLR293C; L8003.19; GTP-binding nuclear protein GSP1/CNR1; Chromosome stability protein 17; GTPase Ran homolog; Genetic suppressor of PRP20-1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
2-219
Target Protein Sequence
SAPAANGEV PTFKLVLVGD GGTGKTTFVK RHLTGEFEKK YIATIGVEVH PLSFYTNFGE IKFDVWDTAG QEKFGGLRDG YYINAQCAII MFDVTSRITY KNVPNWHRDL VRVCENIPIV LCGNKVDVKE RKVKAKTITF HRKKNLQYYD ISAKSNYNFE KPFLWLARKL AGNPQLEFVA SPALAPPEVQ VDEQLMQQYQ QEMEQATALP LPDEDDADL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Essential for cell viability. By analogy with Ras, Ran may be activated when GTP is exchanged for bound GDP by RCC1 and inactivated when GTP is hydrolyzed by Ran upon activation by RanGAP1.
Gene References into Functions
  1. Cex1p recruits Rna1p from the cytoplasm to the nuclear pore complex and facilitates Rna1p activation of the GTPase activity of Gsp1p by enabling Rna1p to gain access to Gsp1p-GTP bound to the Los1p-tRNA-Gsp1p-GTP export complex. PMID: 22008473
  2. Study proved that the additional beta-wedge in Prp20p is essential for the interaction between Prp20p and Gsp1p. PMID: 21093592
  3. Thus, the Gtr1p-Gtr2p cycle was suggested to regulate the Ran cycle through Yrb2p. PMID: 16143306
  4. Results suggest that the GSP1 pathway could regulate proper telomeric function in yeast through Sir4p. PMID: 16956377
Subcellular Location
Nucleus.
Protein Families
Small GTPase superfamily, Ran family
Database Links

KEGG: sce:YLR293C

STRING: 4932.YLR293C

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