Recombinant Schizosaccharomyces pombe ADP-ribosylation factor 6(arf6)

Code CSB-YP001996SXV
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Source Yeast
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Code CSB-EP001996SXV
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Source E.coli
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Code CSB-EP001996SXV-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001996SXV
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Source Baculovirus
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Code CSB-MP001996SXV
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names arf6
Uniprot No. Q9Y7Z2
Alternative Names arf6; SPBC1539.08ADP-ribosylation factor 6
Species Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region 2-184
Target Protein Sequence GNSLFKGFS KPFSRLFSNK EMRILMLGLD AAGKTTILYK LKLNQSVVTI PTVGFNVETV TYKNIKFNVW DVGGQDKIRP LWRHYFTGTK GLIFVVDSAD SNRISEARQE LHRIISDREM RDCLLLVLAN KQDLPGALSP AQITDVLQLD KLKDRLWNVQ PTCALTGDGL LEGLAWLSQN AKLK
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
GTP-binding protein that functions as a molecular switch for the activation of 'new end take off' (NETO), a process in which the directions of cell growth change from a monopolar manner to a bipolar manner in fission yeast. Involved in supplying membrane to the growing new end.
Subcellular Location Cell membrane; Peripheral membrane protein. Cell septum. Note=Localizes at both cell ends and presumptive septa in a cell-cycle dependent manner.
Protein Families Small GTPase superfamily, Arf family
Database Links

KEGG: spo:SPBC1539.08

STRING: 4896.SPBC1539.08.1

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