Recombinant Schizosaccharomyces pombe Suppressor of kinetochore protein 1 (skp1)

Code CSB-YP896941SXV
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP896941SXV
MSDS
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Source E.coli
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Code CSB-EP896941SXV-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896941SXV
MSDS
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Source Baculovirus
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Code CSB-MP896941SXV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
skp1; psh1; sph1; SPBC409.05; Suppressor of kinetochore protein 1; P19/Skp1 homolog
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region
1-161
Target Protein Sequence
MSKIKLISSD NEEFVVDQLI AERSMLIKNM LEDVGEINVP IPLPNVSSNV LRKVLEWCEH HKNDLYSGTE EESDIRLKKS TDIDEWDRKF MAVDQEMLFE IVLASNYLDI KPLLDTGCKT VANMIRGKSP EDIRKTFNIP NDFTPEEEEQ IRKENEWAED R
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for cig2 degradation in the G2 and M phases of the cell cycle. Together with pof6, essential for septum processing and cell separation. Involved in mitotic progression, essential for the execution of anaphase B; required for coordinated structural alterations of mitotic spindles and segregation of nuclear membrane structures at anaphase. Involved in the DNA damage checkpoint pathway and maintenance of genome integrity. Component of the RAVE complex which is required for stable assembly of the vacuolar ATPase complex V-ATPase.
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
SKP1 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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