Recombinant Sheep Osteopontin (SPP1)

Code CSB-YP893170SH
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP893170SH
MSDS
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Source E.coli
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Code CSB-EP893170SH-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP893170SH
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP893170SH
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SPP1
Uniprot No.
Alternative Names
SPP1; OPN; Osteopontin; Bone sialoprotein 1; Secreted phosphoprotein 1; SPP-1
Species
Ovis aries (Sheep)
Expression Region
17-278
Target Protein Sequence
LPVK PTSSGSSEEK QLNNKYPDAV ATWLKPDPSQ KQTFLEPQNS VSSEETDDNK QNTLPSKSNE SPEQTDDLDD DDENSQEVNS DDSDDAETPD DSDHSNESHH SDESDEADFP TDIPTIAVFT PPFPTESTND GRGDSVAYGL KSKSKKFRRS NVESPDATEE DFTSHIESEE MHDAPKKTSQ LTDHSEETNS DELPKELTPK AKEESKHSNR IESQENSKLS QEFHSLEDKL DLDHKSEEDK RLKIRISHEL DSVSSEVN
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.; Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Gene References into Functions
  1. hese results suggested that the expression of OPN might be closely related to spermatogenesis and spermatozoa function in Hu sheep. This will be helpful for us to understand how OPN regulate the high reproductive capacity in Hu sheep. PMID: 27514848
  2. examined the interaction of estrogen receptor-alpha (ERalpha), estrogen receptor-related receptor-alpha (ERRalpha), and stone matrix protein osteopontin (OPN) in a hyperoxaluric model PMID: 21186342
Subcellular Location
Secreted.
Protein Families
Osteopontin family
Database Links

KEGG: oas:443058

UniGene: Oar.14771

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