Recombinant Xenopus laevis Protein Wnt-5a(wnt5a)

Code CSB-YP026138XBE
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Source Yeast
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Code CSB-EP026138XBE
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Source E.coli
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Code CSB-EP026138XBE-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP026138XBE
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Source Baculovirus
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Code CSB-MP026138XBE
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names wnt5a
Uniprot No. P31286
Alternative Names wnt5a; Protein Wnt-5a; XWnt-5a
Species Xenopus laevis (African clawed frog)
Expression Region 41-380
Target Protein Sequence VVESSSWWSL GMNPVQMPEV YIIGAQPLCS QLSGLSQGQK KLCQLYQDHM QFIGDGAKTG IKECQYQFRH RRWNCSTVDN TSVFGRVMQI GSRETAFTYA ISAAGVVNAV SRACREGELS TCGCSRAARP KDLPRDWLWG GCGDNLDYGY RFAKEFVDAR EREKIHQKGS YESSRIMMNL HNNEAGRRAV STLADVACKC HGVSGSCSLK TCWLQLADFR KVGDHLKEKY DSAGAMKLNT RGKLVQVNNK FNSPTMNDLV YIDPSPDYCV HNESTGSLGT QGRLCNKTSE GMDGCELMCC GRGYDQFKTV QTERCHCKFH WCCYVKCKKC TEVVDQFACK
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Affects morphogenetic movement.
Gene References into Functions
  1. In midgastrula embryos, Wnt5a, Wnt11, and Wnt11b, but not Wnt3a, acted across many cell diameters to orient Prickle3/Vangl2 complexes away from their sources regardless of their positions relative to the body axis. PMID: 27658614
  2. Xenopus adult stem cells originate from the larval absorptive cells expressing Ror2, which require Wnt5a/Ror2 signaling for their dedifferentiation accompanied by changes in cell morphology. PMID: 25211363
  3. Data show that PAPC signaling via RhoA and Wnt5a/Ror2 activity are required to keep cells aligned in apical-basal orientation during invagination of the ear placode. PMID: 21663658
  4. Data show that Rspo3 binds syndecan 4 and that together they activate Wnt5a/PCP signaling. PMID: 21397842
  5. In an examination of signaling pathways in developing Xenopus lung, wnt5a was expressed in the mesenchyme layer of the entire lungs through stages 39-41. PMID: 20682360
  6. Wnt5a reduces cell surface levels and promotes ubiquitination and degradation of SDC4 in dorsal mesodermal cells from Xenopus gastrulae. PMID: 20639201
  7. Transcriptional regulation of XPAPC by XWnt-5A requires the receptor tyrosine kinase Ror2. PMID: 17488628
  8. Xwnt-5a plays an instructive role in larval tail regeneration via Wnt/JNK signaling PMID: 18977433

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Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Wnt family
Tissue Specificity Found primarily in ectoderm with lower levels of expression in mesoderm. Detected in the head and tail with lower expression in the middle of the embryo. No expression was found in the notochord.
Database Links

KEGG: xla:378689

UniGene: Xl.19997

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