ACTR2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA144588A0HU
Size	US$210
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Image	Western Blot , Positive WB detected in:Jurkat whole cell lysate(30µg), A549 whole cell lysate(30µg), THP-1 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), MCF7 whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Mouse spleen tissue lysate(30µg), Rat kidney lysate(30µg) All lanes: Actin related protein 2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time：10s Overlay Peak curve showing jurkat cells stained with CSB-RA144588A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Target Background

Product Details

Uniprot No.

P61160

Target Names

ACTR2

Alternative Names

Actin like protein 2 antibody; Actin related protein 2 antibody; Actin-like protein 2 antibody; Actin-related protein 2 antibody; ACTR2 antibody; ARP2 actin related protein 2 homolog (yeast) antibody; ARP2 antibody; ARP2/3 COMPLEX antibody; ARP2_HUMAN antibody; OTTHUMP00000159937 antibody; OTTHUMP00000202270 antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human ACTR2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

25C9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

ACTR2, also known as actin-related protein 2, serves as an essential component of the Arp2/3 complex, a molecular machine that drives actin filament nucleation and branching. This protein plays a fundamental role in cytoskeletal dynamics, influencing cellular processes ranging from cell migration and membrane protrusion to endocytosis and intracellular trafficking. For researchers investigating signal transduction pathways and cytoskeletal regulation, reliable detection of ACTR2 provides valuable insights into these interconnected cellular mechanisms.

This recombinant monoclonal antibody, clone 25C9, offers the consistency and reproducibility that demanding experimental workflows require. Generated using recombinant technology with a defined sequence, this rabbit IgG delivers lot-to-lot uniformity that traditional hybridoma-derived antibodies cannot match. Researchers can confidently compare results across extended studies knowing the antibody performance remains stable.

Validation data demonstrates robust performance across multiple applications and sample types. In western blot analysis, the antibody detects a clean band at the predicted 45 kDa molecular weight across diverse human cell lines including Jurkat, A549, THP-1, HepG2, MCF7, and SH-SY5Y cells. Cross-species reactivity extends to rodent samples, with confirmed detection in mouse brain and spleen tissues as well as rat kidney lysate, providing flexibility for researchers working with animal models. Flow cytometry validation in Jurkat cells shows clear positive signal separation from isotype controls, confirming utility for intracellular protein quantification studies.

Whether investigating actin cytoskeleton remodeling, cell motility mechanisms, or broader signal transduction networks, this antibody provides a dependable tool for exploring ACTR2 biology across human and rodent experimental systems.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the pointed end of the daughter actin filament. In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).

Gene References into Functions

We speculate that ACTR2 and MEIS1 might respectively play a role in the pathogenesis of the observed deafness and cardiomyopathy...the patient carrying a 2p14p15 deletion including OTX1 had normal kidneys and genitalia, thus confirming that OTX1 haploinsufficiency is not invariably associated with genitourinary defects. PMID: 28599093
this study identified roles for ARP2 and filopodia in human respiratory syncytial virus -induced cell motility, virus production, and cell-to-cell spread. PMID: 27926942
Coronin3 can regulate gastric cancer (GC) invasion and metastasis through Arp2, and the combination of Coronin3 and Arp2 provides a potential marker for predicting GC prognosis. PMID: 24918434
Neural Wiskott-Aldrich syndrome protein (N-WASP)-mediated p120-catenin interaction with Arp2-Actin complex stabilizes endothelial adherens junctions. PMID: 23212915
Using molecular dynamics simulations and biochemical assays with recombinant Arp2/3 complex, we now show how phosphorylation of Arp2 induces conformational changes permitting activation. PMID: 22125478
ARP2-mediated actin polymerization is regulated by phosphorylation of WASP protein. PMID: 12791263
Coexpression of Arp2 and WAVE2 is correlated with poorer patient outcome. PMID: 16638851
High mRNA levels of Arp2 were significantly associated with liver metastasis of colorectal cancer. PMID: 17459058
Arp2-depleted Jurkat T lymphocytes exhibit defects in spreading on anti-CD3; Arp2-depleted Jurkats exhibit defects in ruffle assembly but not in assembly of mitotic linear protrusions. PMID: 18720401

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Subcellular Location

Cytoplasm, cytoskeleton. Cell projection. Nucleus.

Protein Families

Actin family, ARP2 subfamily

Database Links

HGNC: 169

OMIM: 604221

KEGG: hsa:10097

STRING: 9606.ENSP00000367220

UniGene: Hs.643727

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Target Therapy

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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