ACTR2

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Western Blot
, Positive WB detected in:Jurkat whole cell lysate(30µg), A549 whole cell lysate(30µg), THP-1 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), MCF7 whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Mouse spleen tissue lysate(30µg), Rat kidney lysate(30µg)
All lanes: Actin related protein 2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time：10s

Overlay Peak curve showing jurkat cells stained with CSB-RA144588A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Western blot
All lanes: Actin-related protein 2 antibody at 3μg/ml
Lane 1: Mouse spleen tissue
Lane 2: Jurkat whole cell lysate
Lane 3: MCF-7 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 45, 46 kDa
Observed band size: 45 kDa

Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA001248ESR1HU at dilution of 1:100