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AGR2 Recombinant Monoclonal Antibody

Datasheet
Code CSB-RA986192A0HU
Size US$210
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Image
  • Western Blot
    Positive WB detected in: COLO205 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), A549 whole cell lysate(30µg)
    All lanes: AGR2 antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 20 kDa
    Observed band size: 17 kDa
    Exposure time: 2min
  • Immunofluorescence staining of THP-1 cell with CSB-RA986192A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • Overlay Peak curve showing A549 cells stained with CSB-RA986192A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Uniprot No.
O95994
Target Names
AGR2
Alternative Names
AG 2 antibody; AG 2 protein antibody; AG-2 antibody; AG2 antibody; AG2 protein antibody; AGR 2 antibody; AGR2 antibody; AGR2_HUMAN antibody; Anterior gradient 2 homolog (Xenopus laevis) antibody; anterior gradient 2 homolog antibody; Anterior gradient 2; protein disulphide isomerase family member antibody; Anterior gradient 2; Xenopus; homolog of antibody; Anterior gradient homolog 2 antibody; Anterior gradient protein 2 homolog antibody; Epididymis secretory protein Li 116 antibody; GOB 4 antibody; GOB4 antibody; HAG 2 antibody; hAG-2 antibody; hAG2 antibody; HAG2/R antibody; HEL S 116 antibody; HPC 8 antibody; hPC8 antibody; PDIA17 antibody; Protein disulfide isomerase family A member 17 antibody; secreted cement gland homolog antibody; Secreted cement gland protein XAG 2 homolog antibody; Secreted cement gland protein XAG-2 homolog antibody; Secreted cement gland protein XAG2 homolog antibody; XAG 2 antibody; XAG2 antibody
Species Reactivity
Human
Immunogen
A synthesized peptide derived from human AGR2
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
9H3
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB, IF, FC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IF 1:50-1:200
FC 1:50-1:200
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

AGR2, or Anterior Gradient 2, is a protein disulfide isomerase family member that plays a critical role in protein folding and secretion within the endoplasmic reticulum. This protein has garnered significant research attention due to its elevated expression in various adenocarcinomas and its involvement in cancer cell survival, metastasis, and therapeutic resistance. Understanding AGR2 biology is particularly relevant for researchers investigating tumor progression, ER stress responses, and potential diagnostic or therapeutic targets in oncology.

This recombinant monoclonal antibody against human AGR2 offers the consistency and reliability that demanding experimental workflows require. Produced using recombinant technology with a defined sequence, clone 9H3 eliminates the lot-to-lot variability that can compromise longitudinal studies and reproducibility. The rabbit IgG format, purified by affinity chromatography, ensures high specificity for your target.

Validation across multiple platforms demonstrates this antibody's experimental versatility. Western blot analysis successfully detects AGR2 in COLO205 colorectal carcinoma, MCF-7 breast cancer, and A549 lung adenocarcinoma cell lysates at dilutions ranging from 1:500 to 1:2000.The observed ~17 kDa band is likely due to the cleavage of the signal peptide, potential proteolytic processing, or inherent SDS-PAGE migration discrepancies causing a downward shift from the predicted 20 kDa. Immunofluorescence staining in THP-1 cells reveals clear cellular localization, while flow cytometry analysis in A549 cells shows distinct positive population shifts compared to isotype controls.

Whether you are characterizing AGR2 expression patterns across cancer cell lines, investigating its role in ER homeostasis, or validating it as a biomarker candidate, this antibody provides the multi-application flexibility and recombinant consistency essential for rigorous research.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Customer Reviews and Q&A

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Target Background

Function
Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells. Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. Promotes cell adhesion.
Gene References into Functions
  1. AGR2 silencing could promote cell apoptosis and inhibit cell migration, invasion and chemotherapy resistance of pancreatic cancer cells with the involvement of the ERK/AKT axis PMID: 30055941
  2. Serum AGR2 can be used as a serum marker for clinical prognosis of nasopharyngeal carcinoma. However, serum AGR2 levels could not provide advantages in clinical practice for the differential diagnosis of cancer. PMID: 29269202
  3. The results suggest that MUC13 overexpression and loss of expression of AGR2 may predict the progression of Intraductal papillary mucinous neoplasm and an unfavorable prognosis in patients with Intraductal papillary mucinous neoplasm PMID: 29650332
  4. Cytosolic AGR2 contributed to cell metastasis ascribed to its stabilizing effect on p65 protein, which subsequently activated the NF-kappaB and facilitated epithelial to mesenchymal transition (EMT). PMID: 29410027
  5. Our data confirm AGR2 as a keeper of epithelial phenotypes, showing a positive role of AGR2 in the regulation of the epithelial marker E-cadherin and a negative effect on the mesenchymal markers vimentin and N-cadherin. PMID: 28810836
  6. Authors demonstrated that inhibition of either FOXM1 or AGR2 in human PIMAs inhibited mucinous characteristics, and reduced tumor growth and invasion. FOXM1 is necessary and sufficient to induce mucinous phenotypes in lung tumor cells in vivo. PMID: 29267283
  7. AGR2 is secreted by prostate cancer cells and induces programmed cell death in normal cells PMID: 27283903
  8. secretion and cell surface localization of AGR2 are characteristic of bladder cancer, while expression of AGR2 by itself is not. PMID: 26894971
  9. Obesity induces the expression of the mitochondrial form of arginase ARG2 in pancreatic ductal adenocarcinoma. PMID: 28808255
  10. Overexpression of AGR2 counteracts the phenotypes induced by miR-342-3p. PMID: 29107102
  11. The authors conclude that extracellular AGR2 plays an extracellular role independent of its endoplasmic reticulum function and elucidate this gain-of-function as a novel and unexpected critical extracellular matrix microenvironmental pro-oncogenic regulator of epithelial morphogenesis and tumorigenesis. PMID: 27240165
  12. High AGR2 expression is associated with ovarian cancer. PMID: 28481872
  13. This study demonstrates that AGR2, which is overexpressed in a variety of human cancers and provides a poor prognosis, up-regulates DUSP10 which subsequently inhibits p38 MAPK and prevents p53 activation by phosphorylation. PMID: 26733232
  14. A direct impact of mTOR signalling on AGR2 3'UTR shortening associated with increased protein synthesis was found, which led to the identification of a novel molecular mechanism involved in upregulation of AGR2 levels in mTOR-activated cells via modulating the 3'UTR length of AGR2 mRNA. PMID: 28408318
  15. Suggest elevated AGR2 as a candidate tamoxifen-dependent mechanism of action responsible for increased incidence of endometrial cancer. PMID: 28402678
  16. AGR2 could also be upregulated in hormone-independent manner. AGR2 expression was shown to be significantly increased in HER2 positive breast tumors on both the mRNA and the protein level. PMID: 28238761
  17. Our findings demonstrated recombinant human endostatin-gold nanoparticles might normalize vessels by interfering anterior gradient 2-mediated angiogenesis in metastatic colorectal cancer. PMID: 28714365
  18. All these data clearly showed that anterior gradient protein 2 is highly expressed in breast cancer and can be regarded as a putative biomarker for breast cancer prognosis. PMID: 28671019
  19. AGR2, through interaction with ER-alpha, enhances fulvestrant resistance and IGF-1-induced carcinogenesis in breast cancer cells. PMID: 27063095
  20. AGR2 inhibits TNF-alphainduced Caco-2 cell hyperpermeability by regulating TJ and that this protective mechanism may be promoted by inhibition of NF-kappaB p65-mediated activation of the MLCK/p-MLC signaling pathway. PMID: 28339048
  21. our study showed the important role of AGR2 in regulating EMT of glioblastoma. SDF-1 signaling induced the expression of AGR2 to control the progress of EMT in the progression of glioblastoma. PMID: 26608373
  22. Results show that AGR2 is O-glycosylated upon secretion from human and rat cell lines, and that this form of the protein is released from healthy cells and not as a result of cell lysis. PMID: 26169982
  23. Nearly all NSCLC tumors show AGR2 expression. Lung cancer expression of AGR2 has prognostic value for younger patients. PMID: 26445321
  24. These data together identify the single cysteine of AGR2 as an oxidant responsive moiety that regulates its propensity for oxidation and its monomeric-dimeric state. PMID: 26876500
  25. The prognostic impact of AGR2 expression could be related to treatment outcome in estrogen receptor-positive breast cancers and/or its metastasis-promoting effects. PMID: 25663596
  26. analysis of molecular characteristics, function, and regulation of AGR2 in cancer[review] PMID: 25937245
  27. AGR2 was higher in HNSCC than in normal oral mucosa. High levels were associated with the T category, pathological grade and lymphatic metastasis. AGR2 expression increased in recurring HNSCC after radiotherapy or cisplatin-based chemotherapy. PMID: 25871396
  28. The mechanism linking the IGF-1/insulin signal and AGR2 promoter activation is important. PMID: 25956506
  29. Data show the potential usability of anterior gradient homolog 3 (AGR3) and anterior gradient homolog 2 (AGR2) as biomarkers for blood-based early detection of breast cancer. PMID: 25875093
  30. Our findings, for the first time, describe AGR2 as an important regulator in chemical hypoxia-induced doxorubicin resistance in breast cancer cells PMID: 26079208
  31. Relying on available data and in silico analyses, study shows that AGR2 and AGR3 proteins are co-expressed or uncoupled in context-dependent manners in diverse carcinomas and healthy tissues. PMID: 25666661
  32. High AGR2/LGR5 was associated with poor progression-free survival by multivariate analysis. PMID: 22605983
  33. Data define the importance of AGR2 in breast cancer cell growth and highlight a mechanism where changes in FOXA1 activity obviate the need for ER in the regulation of this gene. PMID: 25100862
  34. Knockdown of anterior gradient 2 expression extenuates tumor-associated phenotypes of SNU-478 ampulla of Vater cancer cells. PMID: 25367337
  35. AGR2 is a novel marker of papillary thyroid carcinoma. PMID: 24976026
  36. AGR2 splice variant transcripts are potential diagnostic biomarkers for the non-invasive detection of PCa using urine exosomes. PMID: 25237833
  37. Expression of anterior gradient 2 is decreased with the progression of human biliary tract cancer. PMID: 25186196
  38. AGR2 has both intracellular and extracellular effects in the intestine PMID: 25111734
  39. Anterior gradient 2 downregulation in a subset of pancreatic ductal adenocarcinoma is a prognostic factor indicative of epithelial-mesenchymal transition. PMID: 25418581
  40. Results indicate that DNA methylation within the AGR2 promoter modulates more aggressive cancer cell phenotypes. PMID: 24920423
  41. Data demonstrates that AGR2 protein has a more dominant action on proteome remodelling rather than transcriptome effects and highlight a dominant effect on p53 pathway attenuation. PMID: 24710632
  42. anterior gradient 2 (AGR2) was overexpressed in human Nasopharyngeal carcinoma (NPC) tissues, particularly in cervical lymph node metastatic nasopharyngeal carcinoma. PMID: 24717913
  43. On the basis of an initial cohort of 37 subjects, urinary AGR2/PSA concentration ratios showed a significant difference (P = 0.026) between noncancer and cancer PMID: 24251762
  44. These data suggest that AGR-2 influence prostate cancer metastasis by regulation of cellular adhesion and apoptosis. PMID: 24587138
  45. AGR2-mediated lung adenocarcinoma metastasis novel mechanism network through repression with interferon coupling cytoskeleton to steroid metabolism-dependent humoral immune response. PMID: 24960290
  46. Increased expression of the AGR2 and ERp57 is associated with cancer. [review] PMID: 24490732
  47. Data indicate that aberrantly elevated anterior gradient 2 (AGR2) expression predicts poor patient outcome. PMID: 24177142
  48. AGR2 is frequently lost in colorectal carcinomas and might be a novel independent prognostic factor for overall patient survival. PMID: 24794000
  49. Prostate cancer biomarkers, AGR2 and CD10, can be used in combination to stratify prostate cancer patients for clinical outcome. PMID: 23348903
  50. overexpression of AGR2 showed a positive correlation with oral tumor metastasis. PMID: 23834814

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Subcellular Location
Secreted. Endoplasmic reticulum.
Protein Families
AGR family
Tissue Specificity
Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid
Database Links

HGNC: 328

OMIM: 606358

KEGG: hsa:10551

STRING: 9606.ENSP00000391490

UniGene: Hs.530009

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