Code | CSB-RA263831A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:200 |
The AGT recombinant monoclonal antibody is produced using protein and DNA recombinant technology. Mice are first immunized with a synthetic peptide derived from human AGT. After a certain period, the spleen of the mice is removed aseptically, and total RNA is extracted from the spleen cells. The RNA is then reversely transcribed into cDNA, which serves as a template for PCR amplification of the AGT antibody gene. The resulting gene is inserted into a vector and transfected into host cells for culture. The AGT recombinant monoclonal antibody is purified from the cell culture supernatant by affinity chromatography. It is thoroughly verified and can be used for human AGT protein detection in ELISA and IHC experiments.
AGT is a protein that is produced by the liver and secreted into the bloodstream. It plays a crucial role in the renin-angiotensin system (RAS), which is responsible for regulating blood pressure, fluid, and electrolyte balance in the body. AGT expression is also regulated by a number of factors, including hormones such as estrogen, insulin, and glucocorticoids, as well as cytokines and growth factors. Dysregulation of AGT expression has been implicated in the pathogenesis of a variety of cardiovascular and renal diseases, including hypertension, heart failure, and chronic kidney disease.
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