Product Details

Uniprot No.

Target Names

Alternative Names

40 kDa proline rich AKT substrate antibody; 40 kDa proline-rich AKT substrate antibody; AKT1 S1 antibody; AKT1 substrate 1 (proline rich) antibody; AKT1 substrate 1 antibody; AKT1S 1 antibody; AKT1S1 antibody; AKTS1_HUMAN antibody; Lobe antibody; MGC2865 antibody; PRAS 40 antibody; PRAS antibody; PRAS40 antibody; Proline rich akt substrate antibody; Proline rich Akt substrate 40 kDa antibody; Proline-rich AKT1 substrate 1 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human AKT1S1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

17A2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

AKT1S1, commonly known as PRAS40, serves as a critical substrate of AKT1 and functions as a key regulatory component of the mTORC1 signaling pathway. This 40 kDa proline-rich protein acts as an inhibitor of mTORC1 activity, and its phosphorylation status directly influences cellular processes including protein synthesis, cell growth, and survival. Given its involvement in nutrient sensing and growth factor signaling, AKT1S1 has become an important target for researchers investigating metabolic regulation, cancer biology, and cellular stress responses.

This recombinant monoclonal antibody, clone 17A2, offers the reproducibility and consistency that demanding experimental workflows require. Developed using recombinant technology with a defined sequence, this rabbit IgG antibody eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived reagents, ensuring your results remain comparable across extended studies. The antibody was raised against a synthesized peptide derived from human AKT1S1 protein and purified by affinity chromatography for optimal specificity.

Validation studies demonstrate reliable performance across multiple applications. Immunohistochemistry testing in paraffin-embedded human tissues, including skin and breast cancer samples, shows clear target detection using citrate buffer antigen retrieval at 1:100 dilution. Flow cytometry analysis in HeLa cells confirms intracellular detection capability, with distinct signal separation from isotype controls when cells are fixed and permeabilized. These validated applications provide flexibility for researchers examining AKT1S1 expression in both tissue sections and cell-based assays.

Whether you are investigating mTORC1 pathway dynamics, characterizing tumor samples, or exploring metabolic signaling mechanisms, this antibody delivers the performance and consistency needed for meaningful cell biology research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.

Gene References into Functions

The Akt-PRAS40 pathway is activated by uric acid, which inhibits autophagy and recapitulates the uric acid-induced proinflammatory cytokine phenotype. PMID: 28484006
crystal structures of RAPTOR-TOS motif complexes that define the determinants of TOS recognition, of an mTOR FKBP12-rapamycin-binding (FRB) domain-substrate complex that establishes a second substrate-recruitment mechanism, and of a truncated mTOR-PRAS40 complex that reveals PRAS40 inhibits both substrate-recruitment sites PMID: 29236692
Study found that activated PRAS40 acts not only as a regulator of TGFA-triggered exosome secretion but also as a common regulator of distinct microenvironmental and oncogenic signal-triggered exosome secretion in both normal and tumor cell types. PRAS40 is the first regulator identified for stress-induced exosome secretion. PMID: 28674187
PRAS40 was downregulated in the DU145 cells following MYO6 knockdown. PMID: 27431378
Phosphorylation of S202/203 of AKT1S1 by PKM2 released AKT1S1 from raptor and facilitated its binding to 14-3-3, resulted in hormonal- and nutrient-signals independent activation of mTORC1 signaling and led accelerated oncogenic growth and autophagy inhibition in cancer cells. PMID: 26876154
The frameshift mutation detected in the current study would result in a premature stop of amino-acid synthesis in AKT1S1 protein and hence resembles a typical loss-of-function mutation. PMID: 25648575
Supporting this idea, we identified a downstream target of the TGFb-miR-96 signaling pathway to be AKT1S1 mRNA, whose translated protein is a negative regulator of mTOR kinase. PMID: 25531317
This review discusses the role of PRAS40 and possible feedback mechanisms, and alterations in AKT/PRAS40/mTOR signaling that have been implicated in the pathogenesis of tumor progression. [review] PMID: 26003731
Findings indicate that dual phosphorylation of PRAS40 by Akt and mTORC1 promotes formation of a nuclear-specific PRAS40- and RPL11-containing complex distinct from mTORC1 that inhibits the RPL11-HDM2-p53 pathway. PMID: 24704832
PRAS40 as a regulator of insulin sensitivity in hSkMC PMID: 24576065
PRAS40 contains a functional nuclear export signal. Furthermore, enforced nuclear accumulation of PRAS40 impairs insulin action, thereby substantiating the function of this protein in the regulation of insulin sensitivity. PMID: 23712034
Data suggest that PRAS40 modulates insulin action in skeletal muscle; knockdown of PRAS40 inhibits insulin action in cultured myotubes and is associated with up-regulation of IRS1 (insulin receptor substrate 1) degradation via proteasome proteolysis. PMID: 23460019
WISP1 governs PRAS40 by sequestering PRAS40 intracellularly through post-translational phosphorylation. PMID: 22873724
study found tuberin and PRAS40 to be potent anti-apoptotic gatekeepers in early stem-cell differentiation; data allow new insights into the regulation of early stem-cell maintenance and differentiation and identify a new role of the tumor suppressor tuberin and the oncogenic protein PRAS40 PMID: 22090422
This review summarizes the regulation and potential function(s) of PRAS40 in the complex Akt- and mTOR-signaling network in health and disease--{REVIEW} PMID: 22354785
our findings suggest that PRAS40 promotes the development of ESFT PMID: 22241085
PRAS40 is known for its ability to regulate the mammalian target of rapamycin complex 1 kinase activity, possessing a key regulatory role at the cross point of signal transduction pathways activated by growth factor receptors PMID: 21906675
phosphorylation of PRAS40 is critical for the activation of mTOR in CNI-induced VEGF overexpression and renal cancer progression. PMID: 21886838
dissociation of PRAS40 from insulin-stimulated 4E-BP protein binding to mTORC1 and enhanced mTORC1 substrate binding results from Akt and mTORC1 activation and makes little or no contribution to mTORC1 signaling PMID: 21914810
PIM1-activated pPRAS40, AKT-activated pFOXO3a, and their complex formation with 14-3-3 could be key regulators of the radiation-induced radioresistance in NSCLC cells PMID: 21910584
PRAS40 is an important regulator of insulin sensitivity of the Akt-mTOR pathway and a potential target for the treatment of cancers, insulin resistance and hamartoma syndromes. PMID: 17277771
PRAS40 inhibits cell growth, S6K1 phosphorylation, and rheb-induced activation of the mTORC1 pathway PMID: 17386266
PRAS40 regulates mTORC1 kinase activity by functioning as a direct inhibitor of substrate binding. PMID: 17510057
PRAS40 acts downstream of mTORC1 but upstream of its effectors, such as S6K1 and 4E-BP1 PMID: 17604271
activation of mTORC1 signalling by phorbol esters does not require PRAS40 to be phosphorylated at Thr(246), bind to 14-3-3 or be released from mTORC1. PMID: 18215133
after mTORC1 kinase activation by upstream regulators, PRAS40 is phosphorylated directly by mTOR, thus contributing to the relief of PRAS40-mediated substrate competition. PMID: 18372248

Hide All

Subcellular Location

Cytoplasm, cytosol.

Tissue Specificity

Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293).

Database Links

HGNC: 28426

OMIM: 610221

KEGG: hsa:84335

STRING: 9606.ENSP00000341698

UniGene: Hs.515542

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Code	CSB-RA131536A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA131536A0HU diluted at 1:100 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA131536A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing Hela cells stained with CSB-RA131536A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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AKT1S1 Recombinant Monoclonal Antibody

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