Product Details

Uniprot No.

P09917

Target Names

ALOX5

Alternative Names

Arachidonate 5-lipoxygenase (5-LO) (5-lipoxygenase) (EC 1.13.11.34), ALOX5, LOG5

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human 5 Lipoxygenase

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

1D4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Arachidonate 5-lipoxygenase, commonly known as 5-LO or ALOX5, catalyzes the first committed step in leukotriene biosynthesis, converting arachidonic acid into potent inflammatory mediators. This enzyme plays a central role in inflammatory signaling cascades and has emerged as a significant focus in cancer biology, cardiovascular disease research, and metabolic studies. Understanding ALOX5 expression patterns and regulation provides valuable insights into inflammatory pathways that drive disease progression across multiple therapeutic areas.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human 5-lipoxygenase, offers the reproducibility and consistency that demanding research protocols require. As a sequence-defined reagent produced through recombinant technology, it eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The rabbit IgG format, purified by affinity chromatography, ensures high specificity for your target while maintaining compatibility with standard detection systems.

Validation studies demonstrate reliable performance in immunohistochemistry applications using human tissue samples. Testing in paraffin-embedded human lung and spleen tissues at 1:100 dilution, with citrate buffer antigen retrieval and overnight primary incubation at 4°C, produced clear specific staining when detected with HRP-conjugated anti-rabbit polymer and DAB visualization. The recommended working range of 1:50 to 1:200 for IHC provides flexibility to optimize signal intensity for your specific tissue types and experimental conditions. The antibody is also validated for ELISA applications.

Whether you are investigating leukotriene-mediated inflammation in cardiovascular disease models, exploring ALOX5 as a potential oncology target, or characterizing metabolic signaling pathways, this antibody delivers the consistent performance needed to generate reliable, publication-quality data.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Catalyzes the oxygenation of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) to 5-hydroperoxyeicosatetraenoate (5-HPETE) followed by the dehydration to 5,6- epoxyeicosatetraenoate (Leukotriene A4/LTA4), the first two steps in the biosynthesis of leukotrienes, which are potent mediators of inflammation. Also catalyzes the oxygenation of arachidonate into 8-hydroperoxyicosatetraenoate (8-HPETE) and 12-hydroperoxyicosatetraenoate (12-HPETE). Displays lipoxin synthase activity being able to convert (15S)-HETE into a conjugate tetraene. Although arachidonate is the preferred substrate, this enzyme can also metabolize oxidized fatty acids derived from arachidonate such as (15S)-HETE, eicosapentaenoate (EPA) such as (18R)- and (18S)-HEPE or docosahexaenoate (DHA) which lead to the formation of specialized pro-resolving mediators (SPM) lipoxin and resolvins E and D respectively, therefore it participates in anti-inflammatory responses. Oxidation of DHA directly inhibits endothelial cell proliferation and sprouting angiogenesis via peroxisome proliferator-activated receptor gamma (PPARgamma). It does not catalyze the oxygenation of linoleic acid and does not convert (5S)-HETE to lipoxin isomers. In addition to inflammatory processes, it participates in dendritic cell migration, wound healing through an antioxidant mechanism based on heme oxygenase-1 (HO-1) regulation expression, monocyte adhesion to the endothelium via ITGAM expression on monocytes. Moreover, it helps establish an adaptive humoral immunity by regulating primary resting B cells and follicular helper T cells and participates in the CD40-induced production of reactive oxygen species (ROS) after CD40 ligation in B cells through interaction with PIK3R1 that bridges ALOX5 with CD40. Also may play a role in glucose homeostasis, regulation of insulin secretion and palmitic acid-induced insulin resistance via AMPK. Can regulate bone mineralization and fat cell differentiation increases in induced pluripotent stem cells.

Gene References into Functions

our work shows that ALOX5 plays a moderate anti-tumor role and functions as a drug sensitizer, with a therapeutic potential, in MLL-rearranged AML. PMID: 28500307
Study shows that higher COX-2 and ALOX5 expression in colorectal cancer (CRC) tissues was correlated with poorer prognosis in patients with CRC. Also, MiR-216a-3p was shown to directly bind to there 3'-UTR and inversely regulates their protein levels modulating CRC cell proliferation. PMID: 28786533
Indicate a potential protective role of ALOX5AP and 5-arachidonate lipoxygenase gene in diabetes type 2 pathogenesis. PMID: 29392977
Our data reveal that 5-LO, which is required for leukotriene production and subsequent T cell recruitment, is downregulated in TAMs through Mer tyrosine kinase-dependent recognition of apoptotic cancer cells. PMID: 29229677
the influence of TGFbeta/SMADs on MLL- and MLL-AF4-mediated 5-LO promoter activation PMID: 28803964
The knockdown of arachidonate 5-lipoxygenase (Alox5) gene can induce the decreased levels of bcl/abl mRNA and BCR/ABL fusion protein in the K562/ADM cells and increased apoptosis rate. PMID: 29169426
Data suggest that the co-carcinogens benzidine and hydrogen peroxide induce expression of ALOX5 mRNA and protein in tracheobronchial epithelial cells; the co-carcinogens decrease cell proliferation but enhance apoptosis, actions inhibited by knockdown of ALOX5 by RNA interference. Benzidine appears to undergo metabolic activation to benzidine diimine by ALOX5. PMID: 25001243
This study revealed that epistatic interaction among the ALOX5, ALOX5AP and MPO genes played a significant role in vulnerability to ischemic stroke. PMID: 29041000
The anticancer effects by 13'-COOHs appeared to be partially independent of inhibition of COX-2/5-LOX. Using liquid chromatography tandem mass spectrometry, we found that 13'-COOHs increased intracellular dihydrosphingosine and dihydroceramides after short-time incubation in HCT-116 cells, and enhanced ceramides while decreased sphingomyelins during prolonged treatment PMID: 27016075
ROS production induced by the 5-LO pathway mediates the anti-cancer effects of docosahexaenoyl ethanolamide and N-arachidonoyl-L-alanine on head and neck squamous cell carcinoma cells. PMID: 27411387
Specific inhibitors of COX-2 and 5-LOX decreased formation of HKD2 and HKE2 Platelets did not form HKs from exogenous 5S-hydroxyeicosatetraenoic acid, implying that COX-1 is not involved PMID: 28096231
The observation that the coexpression of FLAP with a subset of the 5-LOX mutants restores 5-LOX-wild-type (wt)-like levels of products formed in intact cells suggests a physical protein-protein interaction, beyond colocalization, of 5-LOX and FLAP. PMID: 26842853
Polymorphisms in the 5-Lipoxygenase is associated with Incident Myocardial Infarction. PMID: 27893808
our results define Alox5 as a key genetic effector of JAK2V617F in driving polycythemia vera PMID: 27784744
Adipose tissue eicosapentaenoic acid and arachidonic acid and the ALOX-5 tandem repeat polymorphism did not significantly interact to affect the risk of myocardial infarction. PMID: 28566527
coexpression of the isoforms inhibited or stimulated 5-LO-WT expression in transiently and stably transfected HEK293T cells suggesting that the isoforms have other functions than canonical leukotriene biosynthesis PMID: 28257804
a novel putative protein isoform of human 5-LO that lacks exon 4, termed 5-LODelta4, was identified. PMID: 27855198
Oxidative stress decreased the levels of PNPLA2 transcripts with no effect on ALOX5 expression. Exogenous additions of P1 peptide or overexpression of the PNPLA2 gene decreased both LTB4 levels and death of RPE cells undergoing oxidative stress. PMID: 27635633
Dimethyl ester of bilirubin exhibits anti-inflammatory activity through inhibition of secretory phospholipase A2, lipoxygenase and cyclooxygenase. PMID: 27060751
Copy number variation in ALOX5 is associated with NSAIDs-induced urticaria and/or angioedema. PMID: 26959713
This study identified a SNP (rs10507391) in ALOX5 gene as a novel genetic risk factor for Alzheimer's disease and body mass index. PMID: 26944113
ALOX5 gene variants do not appear to be related to clinical CHD events or subclinical atherosclerosis regardless of bioavailable enzyme substrate levels in multiethnic cohort. PMID: 27025886
our data suggest that the inhibition of both COX-2/5-LOX may be an effective therapeutic approach for colon cancer management, particularly for those patients with high expression of COX-2/5-LOX PMID: 26707712
we identified differences in the frequency distribution in the Tibetan population located in the ALOX5 , VKORC1 and PTGS2 genes PMID: 26505400
AF4 and AF4-MLL mediate transcriptional elongation of 5-lipoxygenase mRNA by 1, 25-dihydroxyvitamin D3. PMID: 26329759
the Alox-5 gene might play a role in the differentiation of multiple drug resistant and non-resistant erythroleukemic cell lines PMID: 26852002
These results suggest that COX-2 and 5-LO play roles in tumorigenesis and the progression of primary glioblastoma and that the co-expression pattern of COX-2/5-LO may be used as an independent prognostic factor in this disease. PMID: 26334317
Increased PUFA Content and 5-lipoxygenase pathway expression are associated with subcutaneous adipose tissue inflammation in obese women with type 2 diabetes. PMID: 26378572
The study shows that 5-lipoxygenase (5-LO) in B cells is phosphorylated on Ser523 and demonstrates for the first time a chemical difference between 5-LO in myeloid cells and B cells. PMID: 26210919
Study identified a novel splice variant of 5-LO consisting of 139 amino acids due to premature stop. It was found to be expressed in HepG2 cells only, raising the possibility for putative role in liver cancer development. PMID: 25218842
GSAP cleavage via caspase-3 is regulated and depend upon the availability of 5-Lipoxygenase in Alzheimer's disease. PMID: 26076991
investigated potential mechanisms by which 5-LO13 interferes with 5-LO product biosynthesis in transfected HEK293 cells PMID: 26173130
Two of the most potent/selective inhibitors (HIR-303 and HIR-309) were reductive inhibitors and were potent against 5-LOX in human whole blood, indicating that isoflavans can be potent and selective inhibitors against human leukocyte 5-LOX PMID: 25359714
The study explores the substrate access portal of 5-Lipoxygenase. PMID: 26427761
involvement of lipoxygenase pathways in TNF-alpha-induced production of cytokines and chemokines PMID: 25229347
Report no association between ALOX5 SNPs and atherosclerotic plaque phenotypes. PMID: 25721704
THE 5-LOX interface involving the four cysteines 159, 300, 416 and 418 is important for the translocation to the nuclear membrane and the colocalization with FLAP. PMID: 26327594
Genotoxic stress induces the ALOX5 mRNA and protein expression in a p53-dependent manner. PMID: 26070487
Results suggest that the level of 5-LOX expression was increased in pancreatic cancer tissues and may be related to lymph node metastasis and TNM stage. PMID: 25483364
Low leukotriene B4 receptor 1 leads to ALOX5 downregulation at diagnosis of chronic myeloid leukemia. PMID: 25193960
5-LOX inhibition reduced apoptotic death, restored the initial IL-2/INF-gamma ratio, and more importantly reverted micro-calpain activation induced by simulated microgravity. PMID: 25309925
contributes to inflammatory microenvironment of precancerous pancreatic lesions PMID: 25454978
Increased metabolites of 5-lipoxygenase from hypoxic ovarian cancer cells promote tumor-associated macrophage infiltration. PMID: 24662827
These findings indicate that the oncogenic function of c-Myc in prostate cancer cells is regulated by 5-Lox activity, revealing a novel mechanism of 5-Lox action PMID: 25540201
Human cytomegalovirus (HCMV) induced up-regulation of 5-lipoxygenase in both ex vivo HCMV-infected placental explants and human umbilical vein endothelial cells (HUVEC) PMID: 24746852
Jurkat T cells overexpressing 5-LO failed to activate PPARgamma in macrophages, while their 5-LO overexpressing apoptotic counterparts did. PMID: 24036216
Polymorphisms within FLT3, EGFR, NEIL3, and ALOX5 may contribute to the occurrence of GBM. PMID: 24005813
5-LO disruption improves wound healing and alters fibroblast function by an antioxidant mechanism based on HO-1 induction. PMID: 24226420
The changes of LTB4 concentration in serum and the mRNA expression level of 5-LO in decidua may play an important role in the success and maintenance of a healthy pregnancy. PMID: 23572152
The 5-LOX/LTC4 /CysLT1 signaling pathway regulates EGF-induced cell migration by increasing Tiam1 expression. PMID: 24350867

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Subcellular Location

Cytoplasm. Nucleus matrix. Nucleus membrane; Peripheral membrane protein. Cytoplasm, perinuclear region. Cytoplasm, cytosol. Nucleus envelope. Nucleus intermembrane space.

Protein Families

Lipoxygenase family

Database Links

HGNC: 435

OMIM: 152390

KEGG: hsa:240

STRING: 9606.ENSP00000363512

UniGene: Hs.89499

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Code	CSB-RA198316A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA198316A0HU diluted at 1:100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA198316A0HU diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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ALOX5 Recombinant Monoclonal Antibody

Product Details

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Target Background