Product Details

Uniprot No.

Target Names

ALYREF

Alternative Names

Ally of AML-1 and LEF-1 antibody; Ally of AML1 and LEF1 antibody; ALY antibody; ALY/REF antibody; Aly/REF export factor antibody; BEF antibody; bZIP enhancing factor antibody; bZIP-enhancing factor BEF antibody; REF antibody; THO complex 4 antibody; THO complex subunit 4 antibody; Tho4 antibody; thoc4 antibody; THOC4_HUMAN antibody; Transcriptional coactivator Aly/REF antibody; Transcriptional coactivator antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human ALYREF

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8F12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

ALYREF, also known as THO complex subunit 4, serves as a critical component of the TREX complex that couples transcription with mRNA export from the nucleus. This multifunctional protein acts as a transcriptional coactivator and plays essential roles in mRNA processing, making it a valuable target for researchers investigating gene expression regulation, nuclear export mechanisms, and RNA biology.

This recombinant monoclonal antibody, developed in rabbit using clone 8F12, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and produced recombinantly, researchers can expect reliable performance across experiments and over time, eliminating the lot-to-lot variability that can complicate long-term studies or multi-site collaborations.

Validation studies demonstrate robust performance across multiple applications. In western blot analysis, the antibody successfully detects ALYREF in diverse human cell lines including HEK293, HeLa, NTERA-2, A549, and HL-60 whole cell lysates at dilutions ranging from 1:500 to 1:2000. The observed band size of approximately 30 kDa, slightly higher than the predicted 27 kDa molecular weight, likely reflects post-translational modifications such as phosphorylation, which is known to regulate ALYREF function. Flow cytometry validation in HepG2 cells confirms the antibody's utility for intracellular detection applications, with recommended dilutions between 1:50 and 1:200.

The unconjugated format provides flexibility for researchers to pair this antibody with their preferred detection systems. Whether investigating mRNA export pathways, characterizing the TREX complex, or exploring ALYREF's role in transcriptional regulation, this antibody delivers the specificity and consistency needed for meaningful experimental outcomes.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Export adapter involved in nuclear export of spliced and unspliced mRNA. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NFX1 pathway). Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm. TREX recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production; ALYREF/THOC4 mediates the recruitment of the TREX complex to the intronless viral mRNA. Required for TREX complex assembly and for linking DDX39B to the cap-binding complex (CBC). In conjunction with THOC5 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in the nuclear export of intronless mRNA; proposed to be recruited to intronless mRNA by ATP-bound DDX39B. Involved in transcription elongation and genome stability. Involved in mRNA export of C5-methylcytosine (m5C)-containing mRNAs: specifically recognizes and binds m5C mRNAs and mediates their nucleo-cytoplasmic shuttling.; Acts as chaperone and promotes the dimerization of transcription factors containing basic leucine zipper (bZIP) domains and thereby promotes transcriptional activation.

Gene References into Functions

Dysregulation of ALYREF-mediated mRNA export upon NSUN2 depletion could be restored by reconstitution of wild-type but not methyltransferase-defective NSUN2. PMID: 28418038
ALYREF mainly binds to the 5' and the 3' regions of the mRNA. ALYREF is likely recruited to mRNAs by PABPN1 and the 3' processing machinery. PMID: 28934468
The competition between hMTR4 and ALYREF determines exosome recruitment and functions in creating balanced nuclear RNA pools for degradation and export. PMID: 28801509
small hairpin RNA-mediated down-regulation of TAP or Aly reduced nuclear export of HDAg-L and assembly of HDV virions. Furthermore, a peptide, TAT-HDAg-L(198-210), containing the 10-amino acid TAT peptide and HDAg-L(198-210), inhibited the interaction between HDAg-L and TAP and blocked HDV virion assembly and secretion. PMID: 27807029
A short conserved motif in ALYREF directs cap- and exon junction complex-dependent assembly of export complexes on spliced mRNAs. PMID: 26773052
the data provides the first site-specific description of how viral mRNA is locked by a herpes viral adaptor protein in complex with cellular ALYREF, giving herpesvirus access to the cellular mRNA export machinery PMID: 24550725
a spatial map is produced in living cells of the sites for the interaction of two TREX subunits, Alyref and Chtop, with Nxf1. PMID: 23826332
ALY is a novel E2F2-interacting protein and a relevant modulator of E2F-responsive gene expression. PMID: 23297349
The interaction of the cellular export adaptor protein Aly/REF with ICP27 contributes to the efficiency of herpes simplex virus 1 mRNA export. PMID: 23637401
High ALY expression is associated with lymph node metastasis in oral squamous cell carcinoma. PMID: 23242234
Authors propose that Aly, THO and UAP56 form a highly integrated unit to associate with the spliced mRNA and function in mRNA export. PMID: 23222130
REF/Aly-ORF57 interaction is not essential for KSHV lytic replication but may contribute to target RNA stability independent of effects on RNA export, suggesting a novel role for REF/Aly in viral RNA metabolism. PMID: 22761374
When the TREX components UAP56 or Aly are knocked down, spliced mRNA, as well as total polyA+ RNA, accumulates in nuclear speckle domains. PMID: 20981025
Results describe the subcellular localization of ICP27 and its colocalization with cellular RNA export factors Aly/REF and TAP/NXF1. PMID: 20015986
suggest a central role for ALY in T-cell enhancer function and oncogene activation PMID: 17229714
Aly is a physiological target of nuclear PI3K signaling, which regulates its subnuclear residency, cell proliferation, and mRNA export activities through nuclear Akt phosphorylation and phosphoinositide association. PMID: 18562279
Adaptor Aly functions in the Tap-p15-mediated nuclear export of HSP70 mRNA. PMID: 19165146
The authors demonstrate that ORF57 recruits several members of hTREX, namely Aly, UAP56 and hTHO-complex proteins, onto the viral mRNAs to assemble an export-competent ribonucleoprotein particle. PMID: 19264631
TAP/NXF1, but not Aly/REF, is required for RNA export during HSV-1 infection. PMID: 19369354
These results indicate that ICP27 RGG box methylation regulates interaction ALY and SRPK1 proteins. PMID: 19553338

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Subcellular Location

Nucleus. Nucleus speckle. Cytoplasm.

Protein Families

ALYREF family

Tissue Specificity

Expressed in a wide variety of cancer types.

Database Links

HGNC: 19071

OMIM: 604171

KEGG: hsa:10189

STRING: 9606.ENSP00000421592

UniGene: Hs.534385

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Code	CSB-RA146127A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: HEK293 whole cell lysate(20µg), Hela whole cell lysate(20µg),NTERA-2 whole cell lysate(20µg), A549 whole cell lysate(20µg), HL-60 whole cell lysate(20µg) All lanes: THOC4 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 27 kDa Observed band size: 30 kDa Exposure time：1min Overlay Peak curve showing HepG2 cells stained with CSB-RA146127A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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ALYREF Recombinant Monoclonal Antibody

Product Details

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Target Background