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ANXA4 Recombinant Monoclonal Antibody

Datasheet
Code CSB-RA001845MA1HU
Size US$210
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Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF7 whole cell lysate, U251 whole cell lysate, PC-3 whole cell lysate, Mouse Liver tissue lysate
    All lanes: ANXA4 antibody at 1:500
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 36 kDa
    Observed band size: 36 kDa
  • Overlay Peak curve showing Hela cells stained with CSB-RA001845MA1HU (red line) at 1:800. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,017 events was performed.
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Product Details

Uniprot No.
P09525
Target Names
ANXA4
Alternative Names
35 beta calcimedin antibody; 35-beta calcimedin antibody; AIV antibody; Annexin 4 antibody; Annexin A4 antibody; Annexin IV (placental anticoagulant protein II) antibody; Annexin IV antibody; Annexin IV placental anticoagulant protein II antibody; Annexin-4 antibody; AnnexinA4 antibody; AnnexinIV antibody; ANX 4 antibody; ANX A4 antibody; ANX4 antibody; ANXA 4 antibody; ANXA4 antibody; ANXA4 protein antibody; ANXA4_HUMAN antibody; Carbohydrate binding protein P33/P41 antibody; Carbohydrate-binding protein p33/p41 antibody; Chromobindin 4 antibody; Chromobindin-4 antibody; Chromobindin4 antibody; DKFZp686H02120 antibody; Endonexin I antibody; EndonexinI antibody; Lipocortin IV antibody; LipocortinIV antibody; MGC75105 antibody; OTTHUMP00000160052 antibody; OTTHUMP00000202207 antibody; P32.5 antibody; P33/41 antibody; PAP II antibody; PAP-II antibody; PAPII antibody; PIG 28 antibody; PIG28 antibody; Placental anticoagulant protein II antibody; PP4 X antibody; PP4-X antibody; PP4X antibody; Proliferation inducing gene 28 antibody; Proliferation inducing protein 28 antibody; Protein II antibody; Xanx-4 antibody; ZAP 36 antibody; ZAP36 antibody
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human ANXA4 protein
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Mouse IgG
Clone No.
31D6
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, FC
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
FC 1:20-1:200
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

Annexin A4 belongs to the annexin family of calcium-dependent phospholipid-binding proteins, playing important roles in membrane organization, ion channel regulation, and cellular signaling processes. Also known as placental anticoagulant protein II and lipocortin IV, ANXA4 has garnered significant research interest for its involvement in cardiovascular biology and signal transduction pathways, where it contributes to membrane dynamics and calcium-mediated cellular responses.

This recombinant monoclonal antibody, clone 31D6, offers researchers the consistency and reliability that comes with sequence-defined production. Unlike traditional hybridoma-derived antibodies, recombinant technology ensures lot-to-lot reproducibility, giving you confidence that your experimental conditions will translate seamlessly across studies and collaborations.

Validation studies demonstrate robust performance across multiple applications. In Western blot analysis, the antibody detects a clean band at the predicted molecular weight of 36 kDa across diverse human cell lines including HeLa, HepG2, MCF7, U251, and PC-3, as well as mouse liver tissue lysate, confirming cross-species reactivity between human and mouse samples. This broad sample compatibility provides flexibility when designing comparative studies or working with different model systems. For flow cytometry applications, the antibody has been validated in HeLa cells using intracellular staining protocols, showing clear separation from isotype control, enabling quantitative analysis of ANXA4 expression at the single-cell level.

Whether you are investigating calcium signaling mechanisms, exploring annexin family functions in cardiovascular contexts, or characterizing ANXA4 expression patterns across cell types, this antibody delivers the specificity and reproducibility needed for meaningful results in your research program.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.
Gene References into Functions
  1. ANXA4 expression was increased at both the mRNA and protein level in the drugresistant ovarian cancer cells, and ANXA4 contained a Lewis(y) structure. ANXA4 overexpression can abnormally activate signaling pathways and regulate the expression of a numbers of factors, forming a positive feedback loop to induce the chemoresistance of ovarian cancer cells. PMID: 30066907
  2. Annexin A4 binds to artificial membranes and generates curvature force initiated from free edges, whereas annexin A6 induces constriction force. PMID: 29158488
  3. Taken together, these data indicate that up-regulation of ANXA4 leads to activation of the NF-kappaB pathway and its target genes in a feedback regulatory mechanism via the p65 subunit, resulting in tumor growth in GBC. PMID: 27491820
  4. Upregulation and nuclear translocation of ANXA4 have been observed in the progression of colorectal cancer and ovarian serous carcinoma. Knockdown of ANXA4 attenuated migration in ovarian cancer and breast cancer cells. In contrast, knockdown of ANXA4 increased susceptibility to platinum in ovarian cancer and malignant mesothelioma cells. Review. PMID: 27100483
  5. Study identifies ANXA4 and FLNA as up-regulated in buccal squamous cell carcinoma arising from oral submucous fibrosis. PMID: 27485544
  6. High ANXA4 expression is associated with metastasis of hepatocellular carcinoma. PMID: 26779633
  7. annexin A4 can be regarded as an important molecular marker in triple-negative breast cancer prognosis. PMID: 27650619
  8. role of annexin A4 in cancer PMID: 26048190
  9. Data shows that CYTB and ANXA4 overexpression may be involved in carcinogenesis and histopathological differentiation of ovarian clear cell carcinoma and suggest they may serve as a potential diagnostic biomarkers. PMID: 25633807
  10. These findings indicate that the calcium-binding site in the ANXA4 repeat induces chemoresistance to the platinum-based drug by elevating the intracellular chloride concentration. PMID: 25277200
  11. Fhit delocalizes annexin A4 from plasma membrane to cytosol and sensitizes lung cancer cells to paclitaxel. PMID: 24223161
  12. enhanced expression of Anx A4 confers platinum resistance by promoting efflux of platinum drugs via ATP7A. PMID: 24150977
  13. This study showed that overexpression and nuclear localization of annexin A4 are related to chemoresistance and poor survival in patients with serous papillary ovarian carcinomas. PMID: 23290009
  14. suggest that ANXA4 triggers a signaling cascade, leading to increased epithelial cell proliferation, ultimately promoting carcinogenesis PMID: 22970268
  15. Lower expression of annexin A4 during window of implantation in infertile patients with endometriosis might be associated with the decrease of endometrial receptivity. PMID: 22883517
  16. Eight ANXA4 polymorphisms are significantly associated with the risk of aspirin-exacerbated respiratory disease. PMID: 22847161
  17. that H3K4 tri- and dimethylation play an important role and that JARID1A is the histone-demethylating enzyme responsible for removal of this mark PMID: 21348943
  18. In an Alzheimer model, transgenic mice overexpressing mutant human amyloid precursor protein (APP) show a significant increase of annexin A5 in the brain cortex but not in other organs, including liver, kidney, lung, and intestine. PMID: 20648654
  19. Annexin A4 differentially modulates the NF-kappaB signaling pathway, depending on its interactions with p50 and the intracellular Ca(2+) ion level. PMID: 20237821
  20. The overexpression of Annexin IV may be an ovarian CCC-specific molecular marker. PMID: 19955935
  21. annexin IV plays an important role in the morphological diversification and dissemination of the clear cell renal cell carcinoma PMID: 15145526
  22. This work provides mechanistic insight into how annexin A4 may regulate plasma membrane protein function. PMID: 16687573
  23. ANXA4 transcription and translation are regulated by progesterone. ANXA4 may be important in regulating ion and water transport across the endometrial epithelium. PMID: 16954445
  24. There were no significant differences in plasma annexin IV levels between women with and without antiphospholipid antibodies PMID: 17363042
  25. Enhanced expression of Annexin A4 is associated with clear cell carcinoma of the ovary PMID: 19598262

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Subcellular Location
Zymogen granule membrane; Peripheral membrane protein.
Protein Families
Annexin family
Database Links

HGNC: 542

OMIM: 106491

KEGG: hsa:307

STRING: 9606.ENSP00000377833

UniGene: Hs.422986

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