Code | CSB-RA243116A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:200 |
CUSABIO initiated the immunization process by administering a synthesized peptide derived from the human androgen receptor (AR) to a rabbit, which prompted an immune response. Splenocytes were subsequently isolated from the immunized rabbit and used to extract RNA. The extracted RNA was then reverse-transcribed into cDNA. The AR antibody gene was amplified using degenerate primers and integrated into a vector, which was transfected into a host system for antibody expression. The resulting AR recombinant monoclonal antibodies were purified through affinity chromatography from the cell culture supernatant. Their binding specificity and affinity were validated through ELISA and IHC applications, demonstrating their exclusive recognition of the human AR protein. This AR recombinant monoclonal antibody offers promising prospects for various research.
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