Product Details

Uniprot No.

Q9H1Y0

Target Names

ATG5

Alternative Names

Autophagy protein 5 (APG5-like) (Apoptosis-specific protein), ATG5, APG5L ASP

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide from human ATG5 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

5E10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

ATG5 serves as an essential component of the autophagy machinery, forming a conjugate with ATG12 that facilitates autophagosome membrane elongation. This protein plays a central role in cellular quality control mechanisms, making it a critical target for researchers investigating autophagy regulation, cancer biology, neurodegeneration, and metabolic disorders.

This recombinant monoclonal antibody, derived from clone 5E10, offers the reproducibility and consistency that demanding autophagy research requires. Because the antibody sequence is defined and produced recombinantly, you can expect reliable performance across experiments and eliminate the lot-to-lot variability that can complicate longitudinal studies or multi-site collaborations.

Validation across multiple platforms demonstrates this antibody's versatility in your experimental workflows. Western blot analysis confirms detection in human cell lines including HT-29, HepG2, HEK293T, and SH-SY5Y, as well as mouse NIH/3T3 cells, supporting the stated cross-species reactivity with human and mouse samples. The observed band at approximately 55 kDa, rather than the predicted 32 kDa, reflects detection of the physiologically relevant ATG5-ATG12 conjugate that predominates in cells under normal conditions. Immunohistochemistry staining has been validated in human pancreatic cancer and kidney tissue sections using citrate buffer antigen retrieval, while flow cytometry analysis in 786-O renal carcinoma cells demonstrates clear positive signal separation from isotype controls.

The antibody is supplied in a glycerol-containing buffer optimized for long-term storage stability at -20°C or -80°C. Whether you're characterizing autophagy flux, examining ATG5 expression in disease models, or screening therapeutic compounds that modulate autophagy, this antibody provides a dependable tool for your research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.; May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD.; (Microbial infection) May act as a proviral factor. In association with ATG12, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection. Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for initiation of HCV replication, but not required once infection is established.

Gene References into Functions

variants have role in susceptibility to systemic lupus erythematousus PMID: 29759048
Consistently, forced expression of p53 significantly stimulated ACER2 transcription. Notably, p53-mediated autophagy and apoptosis were markedly enhanced by ACER2. Depletion of the essential autophagy gene ATG5 revealed that ACER2-induced autophagy facilitates its effect on apoptosis. PMID: 28294157
DICER1-AS1 regulates the proliferation, invasion and autophagy of osteosarcoma via miR-30b/ATG5 axis. PMID: 29772430
ATG5 levels were significantly different between chronic total coronary occlusion, acute total coronary occlusion (myocardial infarction) and control groups. PMID: 29455545
Data show that miR-181a binds to the 3' untranslated regions (3'-UTR) of autophagy-related protein 5 (Atg5). PMID: 28930552
Our results suggest that these three genes that are critical components of the autophagy pathway (ATG16L, ATG2B, ATG5) are not significant risk factors among Spanish patients with either pseudoexfoliation syndrome or pseudoexfoliation glaucoma. PMID: 27960588
The results support the association of SNPs in ATG5 and CRP genes with response to adalimumab therapy in Crohn's disease. PMID: 27096233
Atg5-mediated autophagy in proximal epithelial cells is a critical host-defense mechanism that prevents renal fibrosis by blocking G2/M arrest PMID: 27304991
knockdown of autophagy genes ATG5 or ATG7 resulted in reduced hematopoietic stem/progenitor cell frequencies in vitro as well as in vivo. PMID: 26930546
Data suggest that Atg5 expression level in dopaminergic (DA) neurons is decreased and autophagy flux is blocked in zebrafish MPTP model of Parkinson's disease (PD); gene therapy in embryo/larva resulting in human ATG5 overexpression reverses PD pathological features, rescues DA neurons, and restores autophagy flux in DA neurons; thus, ATG5 plays role in neuroprotection. PMID: 28928221
Kaposi sarcoma-associated herpesvirus reduces CAST (calpastatin) and consequently decreases ATG5 expression in both THP-1 monocytoid cells and primary monocytes. PMID: 27715410
These results support ATG5 as a therapeutically relevant dose-dependent rheostat of melanoma progression. PMID: 27464255
Confirm the association of rs548234/ATG5, rs2736340/BLK and rs10516487/BANK1 with systemic lupus erythematosus in Chinese Han and reinforced our hypothesis of their epistasis effect in regulating B-cell signaling in SLE. PMID: 26420661
Neither eosinophils nor neutrophils require ATG5-dependent autophagy for extracellular DNA trap formation. PMID: 28703297
ATG5 rs510432 gene polymorphism might be related to the occurrence of coal workers' pneumoconiosis in a Chinese population. PMID: 28844669
The expression levels of ATG5 and LC3 were associated with classification, histological grade, TNM stage and survival rate in renal carcinoma. PMID: 28274318
the scaffold protein RACK1 is a new ATG5-interacting protein and an important and novel component of the autophagy pathways. PMID: 27325703
UA exerted antiatherosclerosis effects in quails and protected HUVECs from ox-LDL induced cytotoxicity, and the mechanism is associated with increased SIRT1 expression, decreased Atg5 acetylation on lysine residue, and increased autophagy. PMID: 27514536
Genetic polymorphisms of ATG5 and ATG7 could contribute to neutrophilic airway inflammation in the pathogenesis of adult asthma. PMID: 26701229
The authors identified a homozygous missense mutation, changing a conserved amino acid, in ATG5 in two siblings with congenital ataxia, mental retardation, and developmental delay. PMID: 26812546
study established a relationship between trans-eSNPs of ATG5 and lupus nephritis in a northern Han population from China PMID: 26509176
in the presence of 3-MA and Atg5 siRNA, autophagy was inhibited, LC3-II expression decreased and cell apoptosis increased PMID: 26240015
This study demonstrated an association of ATG5 rs573775 with BD and ATG10 rs4703863 with VKH syndrome in a Chinese Han population. PMID: 26747760
provide an insight into a model for swapping ATG5 partners for autophagosome maturation PMID: 25787994
the crystal structures of human ATG5 in complex with an N-terminal domain of ATG16L1 as well as an internal AIR domain of TECPR1, are reported. PMID: 25484072
Taken together, the results demonstrate that ATG5 cleavage provokes apoptosis and represents a molecular link between autophagy and apoptosis PMID: 25481044
Increased frequencies of rare variants of ATG5 were identified in patients with LN and Systemic Lupus Erythematosus compared with healthy controls, highlighting a likely important role of rare ATG5 variants in Chinese SLE patients. PMID: 26039132
Increased expression of ATG5 was significantly correlated with poor overall survival and chemoresistance in gastric cancer. PMID: 25329677
Results indicated that ATG5 variants are associated with NMO but not MS patients, which may provide a clue for further clarifying the autoimmune mechanisms of autophagy-related pathogenesis in NMO. PMID: 24953774
ATG5-expressing cells showed elevated p53 levels and increased p53 activation. PMID: 25032862
Genetic variation in ATG5 is associated with increased susceptibility for thyroid carcinoma. PMID: 24739953
Globular adiponectin restores ethanol-suppressed autophagy-related protein 5 (Atg5) expression in HepG2 cells. PMID: 24582693
both autophagy-related 5 and microtubule-associated protein 1 light chain 3B levels are decreased in patients with melanomas as compared with those with benign nevi PMID: 24300435
Atg5 rs573775 T* allele seems to influence SLE susceptibility, cytokine production and disease features depending on other factors such as functional IL-10 genotype PMID: 24205307
down-regulation of ATG5 contributes to tumorigenesis in early-stage cutaneous melanoma. PMID: 24027027
In dopaminergic cells, Atg5-dependent autophagy acts as a protective mechanism during apoptotic cell death induced by paraquat and MPP but not during rotenone or 6-OHDA toxicity. PMID: 23997112
sublethal concentrations of DNA-damaging drugs, such as etoposide and cisplatin, induce the expression of autophagy-related protein 5, which is both necessary and sufficient for the subsequent induction of mitotic catastrophe. PMID: 23945651
It was concluded taht granulysin induces apoptosis on hematological tumor cells and on cells from B-cell lymphocytic leukemia patients, opening the door to research on its use as a new anti-tumoral treatment. PMID: 24269628
These results suggested that calpain, Bak and Atg5 were molecular links between autophagy and apoptosis and revealed novel aspects of the crosstalk between these two processes. PMID: 23242420
PDCD4 negatively regulates autophagy by targeting ATG5. PMID: 23486359
MIR181A is a novel and important regulator of autophagy and ATG5 is a rate-limiting miRNA target in this effect PMID: 23322078
MAPK14-mediated phosphorylation of ATG5 has a role in inhibiting autophagocytosis PMID: 23235332
Autophagy is differentially induced in prostate cancer LNCaP, DU145 and PC-3 cells via distinct splicing profiles of ATG5. PMID: 23075929
the variant identified in PD patient may change ATG5 protein levels and alter autophagy activities, contributing to Parkinson's disease onset as a risk factor. PMID: 23384565
ATG-5 down-regulation is associated with hepatocellular carcinoma infected with hepatitis C virus. PMID: 23317196
Immunohistochemical analysis of colorectal cancer tissues indicated that increased ATG5 expression is associated with lymphovascular invasion. PMID: 22993366
Genetic variants in ATG5, including a functional promotor variant, are associated with childhood asthma. PMID: 22536318
Results showed no involvement of rs548234 at PRDM1-ATG5 region in the susceptibility or clinical relevance of RA in Chinese Han population. PMID: 22040493
doxorubicin switches protective autophagy in SPP1-depleted cells to apoptosis by calpain-mediated Atg5 cleavage. PMID: 22052905
overexpression of ATG5 gene may be related to autophagy and might play a role in prostate tumorigenesis. PMID: 21995634

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Involvement in disease

Spinocerebellar ataxia, autosomal recessive, 25 (SCAR25)

Subcellular Location

Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein.

Protein Families

ATG5 family

Tissue Specificity

Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.

Database Links

HGNC: 589

OMIM: 604261

KEGG: hsa:9474

STRING: 9606.ENSP00000343313

UniGene: Hs.486063

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Code	CSB-RA050869A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: HT-29 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), HEK293T whole cell lysate(30µg), SH-SY5Y whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg) All lanes: ATG5 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 32 kDa Observed band size: 55 kDa Exposure time：2min IHC image of CSB-RA050869A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA050869A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing 786-O cells stained with CSB-RA050869A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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ATG5 Recombinant Monoclonal Antibody

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Target Background