Product Details

Uniprot No.

Q13315

Target Names

ATM

Alternative Names

Serine-protein kinase ATM (EC 2.7.11.1) (Ataxia telangiectasia mutated) (A-T mutated), ATM

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human ATM

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

3G11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:20-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

ATM (Ataxia Telangiectasia Mutated) is a serine/threonine protein kinase that serves as a master regulator of the DNA damage response, particularly in detecting and signaling double-strand breaks. This critical role in maintaining genomic integrity makes ATM a central focus in cancer research, where its dysfunction contributes to tumor development and influences therapeutic responses to DNA-damaging agents. Understanding ATM expression and localization provides valuable insights into cellular repair mechanisms and potential vulnerabilities in malignant tissues.

This recombinant monoclonal antibody, clone 3G11, offers researchers the consistency and reliability that comes with sequence-defined production. Unlike traditional hybridoma-derived antibodies, recombinant technology ensures that every lot performs identically to the last, eliminating the variability that can compromise longitudinal studies or multi-site collaborations. The antibody was raised in rabbit against a synthetic peptide derived from human ATM, providing defined epitope targeting for reproducible results.

Validation studies demonstrate robust performance across multiple applications. Immunohistochemistry testing in paraffin-embedded human breast cancer tissue reveals clear detection of ATM, making this antibody suitable for investigating ATM status in oncology specimens. Additional IHC validation in human testis tissue confirms reliable staining in normal tissue contexts as well. For cellular analysis, flow cytometry experiments using HeLa cells show distinct positive signal separation from isotype controls, enabling quantitative assessment of ATM expression at the single-cell level.

Whether you are characterizing ATM expression patterns in tumor samples, investigating DNA damage signaling pathways, or screening for ATM status in cancer models, this antibody provides the workflow flexibility needed for epigenetics, nuclear signaling, and oncology research programs.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FBXW7, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, UFL1, RAD9, UBQLN4 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation. Phosphorylates ATF2 which stimulates its function in DNA damage response. Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks.

Gene References into Functions

In mitosis ATM forms a complex with the poly(ADP)ribose polymerase Tankyrase 1, the spindle pole protein NuMA1, and breast cancer protein BRCA1, another crucial DDR player. The complex is required for efficient poly(ADP)ribosylation of NuMA1. PMID: 24553124
ATM was a direct target of miR-181a. PMID: 24531888
A review discusses ATM function and the consequences of its loss during chronic lymphocytic leukemia pathogenesis. PMID: 23906020
Data show that serine-922 of TAX1BP2 protein is the phosphorylation site of ataxia telangiectasia mutated (ATM) kinase. PMID: 24240686
Data indicate that ataxia telangiectasia mutated (ATM) protein phosphorylates BRG1 protein at Ser-721. PMID: 24413084
These results indicate that ATM regulates a sub-set of NF-kappaB dependent genes after a genotoxic stress by direct phosphorylation of p65. PMID: 22715377
Data suggest that expression of cytomegalovirus UL76 up-regulates human interleukin-8 (IL8) expression/secretion in response to DNA damage; both UL76 and human ATM have roles in mechanism of IL8 induction during cytomegalovirus infection. PMID: 24068928
Huntington's disease cells presented a delayed nucleo-shuttling of phosphorylated forms of the ATM kinase. PMID: 24277524
ATM silencing induced partial reduction in levels of Skp2, a component of SCF(Skp2) ubiquitin ligase that controls Cdt1 degradation. PMID: 24280901
Heterozygosity for the ATM rs373759 polymorphism may be a potential risk factor for papillary thyroid cancer. PMID: 23925578
cAMP signaling inhibits radiation-induced ATM activation by PKA-dependent activation of PP2A, and this signaling mechanism augments radiation-induced apoptosis by reducing ATM-dependent activation of NF-kappaB in lung cancer cells. PMID: 24568192
Study found that in human oncogene-transformed and cancer cells, ATM suppressed ARF protein levels and activity in a transcription-independent manner. PMID: 23851489
Tumoral loss of ATM protein was detected more often in patients with a family history of pancreatic cancer than in those without PMID: 24486587
An important role of ATM-mediated Mad1 Serine 214 phosphorylation in mitosis. PMID: 24728176
OCT variants ( OCT1, OCT2 and ATM) were significantly associated with elevated baseline and glucose-induced C-peptide levels in polycystic ovary syndrome PMID: 24533710
The findings implicate an important role of variants in the ATM- CHEK2- BRCA1 axis in modification of the genetic predisposition to papillary thyroid carcinoma and its clinical manifestations. PMID: 24599715
Cuc B also triggers ATM-activated p53-14-3-3-sigma pathways. PMID: 24505404
Data indicate that ATM-depletion can sensitize breast cancer cells to PARP inhibition, suggesting a potential in the treatment of breast cancers low in ATM protein expression/activity, such as those arising in mutant ATM heterozygous carriers. PMID: 24252502
ATM levels were significantly down-regulated in oxaliplatin-resistant colorectal cancer cells. PMID: 24145123
ATM-mediated Snail Serine 100 phosphorylation in response to ionizing irradiation plays an important part in the regulation of radiosensitivity. PMID: 23891091
Our study indicates that the expression of Pim kinases is physiologically related to DNA-PKcs and ATM in ECs. PMID: 22282239
At a low reactive oxygen species condition during genotoxic insult, the ATM/sumoylated-IKKgamma interaction induced NFkappaB activation that resisted JNK-mediated apoptosis. PMID: 24457965
genetic association studies in a population of men in Germany: Data suggest that an SNP near ATM (ataxia telangiectasia mutated protein; rs11212617) is associated with coronary artery disease (but not blood glucose level) in the subjects studied. PMID: 24281401
ATM and ATR each contribute to DNA damage response (DDR) activation caused by BKPyV infection. PMID: 22952448
High ATM expression is associated with breast cancer. PMID: 23857602
Reduced protein expression of ATM is associated with breast carcinoma. PMID: 23117476
ATM protein expression is an independent prognostic marker in sporadic breast cancer. PMID: 24285016
ATM mutation and ATM protein loss had characteristics of old age, distal location of tumor, large tumor size, and histologic intestinal type. PMID: 24324828
ATM/ATR pathway plays an important role in tumor recognition. PMID: 24726882
data confirm previous work showing that Lig3 is required to maintain mtDNA integrity and function, and highlight a new function of ATM in regulating DNA Lig3 stability and consequently mtDNA repair PMID: 24342190
ATM mutations either alone or in combination with 11q deletion lead to demonstrable ATM dysfunction in patients with chronic lymphocytic leukemia. PMID: 23585524
The findings of this study suggested that biallelic ATM-inactivating mutations may present as isolated, generalized dystonia. PMID: 23640770
This study therefore sheds light on the mechanisms underlying AZA resistance, and will enable better understanding of AZA resistance in patients undergoing AZA treatment. PMID: 24680865
NKX3.1 and ATM have a functional interaction leading to ATM activation and then NKX3.1 degradation in a tightly regulated DNA damage response specific to prostate epithelial cells. PMID: 23890999
Activation of H2AX and ATM in varicella-zoster virus infected cells is associated with expression of VZV ORF61 and ORF63. PMID: 24606682
IP7, formed by IP6K2, binds CK2 to enhance its phosphorylation of the Tti1/Tel2 complex, thereby stabilizing DNA-PKcs and ATM. This process stimulates p53 phosphorylation at serine 15 to activate the cell death program. PMID: 24657168
ATM and MDC1 maintain genomic stability not only by controlling the DNA damage response, but also by regulating spindle assembly checkpoint activation, providing an important link between these two essential biological processes. PMID: 24509855
we elucidated the prognostic implications of the expressions of ATM, Chk2, and p53, in gastric carcinoma PMID: 23969480
The regulation of ATM by HDAC enzymes therefore suggests a vital role for HDAC1 and HDAC2 in the DNA damage response. PMID: 23939379
Apoptotic progression is markedly attenuated by ATM gene knockdown through downregulation of caspase-8 and caspase-9. PMID: 24530529
ATM and MAPKAP kinase 2 mediate radiation sensitivity in pancreatic cancer cells via phosphorylation of TRIM29. PMID: 24469230
data provide strong evidences that Aurora-A and BRCA1/2 inversely control the sensitivity of cancer cells to radio- and chemotherapy through the ATM/Chk2-mediated DNA repair networks PMID: 24480460
glioma stem cells were more resistant to radiation compared to glioma cells due to high expression of phosphorylated cell cycle checkpoint proteins, and inhibition of ATM could significantly reduce the radioresistance of glioma stem cells and glioma cells PMID: 23846672
ATM-deficient mice show resistance to hepatocyte cell death. PMID: 23435430
mimosine treatment, ATM blocks S phase entry in response to ROS, which prevents replication fork stalling-induced DNA damage. PMID: 24421316
In Rho 0-Hep G2 cells, which lack mitochondrial DNA and functional mitochondria, ATM failed to respond to hydrogen peroxide, indicating that mitochondria are required for the oxidative activation of ATM. PMID: 24406161
12 pathogenic Atm mutations (1 missense, 4 nonsense, 5 frameshift, 1 splicing, and 1 large genomic deletion) were found in 8 Chinese patients from 5 families. All were novel. No homozygous mutation and founder-effect mutation were found. PMID: 23807571
Heterozygous carriers of c.8851-1G>T (associated with absence of ATM kinase activity) had a stronger radiosensitive phenotype with this assay than heterozygous carriers of p.Asp2708Asn (associated with residual kinase activity). PMID: 23632773
Significant association with PTC was found for rs1801516 (D1853N) in ATM and rs1867277 in the promoter region of FOXE1 (OR = 1.55, 95% CI 1.03, 2.34). PMID: 24105688
Kaposi's sarcoma-associated herpesvirus induces the ATM and H2AX DNA damage response early during de novo infection of primary endothelial cells, which play roles in latency establishment. PMID: 24352470

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Involvement in disease

Ataxia telangiectasia (AT)

Subcellular Location

Nucleus. Cytoplasmic vesicle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

Protein Families

PI3/PI4-kinase family, ATM subfamily

Tissue Specificity

Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.

Database Links

HGNC: 795

OMIM: 208900

KEGG: hsa:472

STRING: 9606.ENSP00000278616

UniGene: Hs.367437

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Code	CSB-RA166706A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA166706A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA166706A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. Overlay histogram showing Hela cells stained with CSB-RA166706A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/110⁶ cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/110⁶ cells) used under the same conditions. Acquisition of >10,000 events was performed.
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ATM Recombinant Monoclonal Antibody

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