Code | CSB-RA166706A0HU |
Size | US$210 |
Order now | |
Image |
|
Have Questions? | Leave a Message or Start an on-line Chat |
Application | Recommended Dilution |
---|---|
IHC | 1:50-1:200 |
FC | 1:20-1:200 |
The ATM recombinant monoclonal antibody is produced using a combination of protein and DNA recombinant technologies. Mice were first immunized with a synthesized peptide taken from human ATM protein. After some time, the mice's spleen cells were collected under aseptic conditions, and total RNA was extracted from these cells. The cDNA obtained by reverse transcription of RNA was then used to PCR amplify the ATM antibody gene. The resulting gene was introduced into a vector, which was then transfected into host cells and cultured. The ATM recombinant monoclonal antibody was then purified from the cell culture supernatant using affinity chromatography. It has been thoroughly validated and is suitable for use in ELISA, IHC, and FC experiments to detect human ATM protein.
The serine-protein kinase ATM protein plays a critical role in DNA damage response and maintenance of genomic stability in cells. When DNA is damaged, ATM protein is activated and recruited to the sites of DNA damage, where it phosphorylates multiple downstream proteins including p53, CHK1, CHK2, NBS1, and BRCA1, which initiate a cascade of events that lead to cell cycle arrest, DNA repair, and cell survival. ATM also promotes the recruitment of DNA repair factors to sites of damage, such as the homologous recombination repair pathway. In addition, ATM is also involved in telomere maintenance, chromatin remodeling, and mitotic spindle checkpoint control.
There are currently no reviews for this product.