ATM, a serine/threonine protein kinase within the PI3K-like protein kinase (PIKKs) family, is central to DNA damage response. Predominantly nuclear, ATM's gene resides on chromosome 11. It springs into action at DNA double strand breaks, phosphorylating proteins like p53 and Chk2, which triggers cell cycle arrest, DNA repair, and apoptosis. ATM is vital for genomic stability and countering cancer, as well as for reactions to radiation and chemotherapeutic agents. The disorder of ATM can result in Ataxia telangiectasia, marked by neurological issues, cancer predisposition, and immune system impairments.
ATM Recombinant Monoclonal Antibody (CSB-RA166706A0HU)
Validated Data
IHC image of CSB-RA166706A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA166706A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Overlay histogram showing Hela cells stained with CSB-RA166706A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106 cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.
The following ATM reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
ATM Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA188639 | Phospho-ATM (Ser1981) Antibody | Human,Mouse | ELISA,WB,IHC |
CSB-PA272996 | ATM (Ab-1981) Antibody | Human,Mouse | ELISA,WB,IF |
CSB-PA020042 | ATM Antibody | Human,Mouse | WB, IHC, ELISA |
CSB-PA020043 | ATM Antibody | Human | IHC, ELISA |
CSB-PA465029 | Phospho-ATM (Ser1981) Antibody | Human,Mouse | WB, ELISA |
CSB-PA612595 | ATM Antibody | Human,Mouse,Rat | IHC,ELISA |
CSB-PA243808 | ATM Antibody | Human,Mouse,Rat | IHC,ELISA |
CSB-MA974761 | ATM Monoclonal Antibody | Human,Mouse,Rat | ELISA,IHC |
CSB-MA429116 | ATM Monoclonal Antibody | Human,Mouse,Rat | ELISA,IHC |
CSB-MA341522 | ATM Monoclonal Antibody | Human,Mouse,Rat | ELISA,IHC |
CSB-RA618770A1981phHU | Phospho-ATM (S1981) Recombinant Monoclonal Antibody | Human | ELISA, IF |
CSB-RA618770A0HU | ATM Recombinant Monoclonal Antibody | Human | ELISA, WB, IP |
CSB-RA166706A0HU | ATM Recombinant Monoclonal Antibody | Human | ELISA, IHC, FC |
ATM Antibodies for Arabidopsis thaliana (Mouse-ear cress)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA885532XA01DOA | ATM Antibody | Arabidopsis thaliana | ELISA, WB (ensure identification of antigen) |
ATM Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP618770HU CSB-EP618770HU CSB-BP618770HU CSB-MP618770HU CSB-EP618770HU-B |
Recombinant Human Serine-protein kinase ATM (ATM), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
ATM Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP726793MO CSB-EP726793MO CSB-BP726793MO CSB-MP726793MO CSB-EP726793MO-B |
Recombinant Mouse Serine-protein kinase ATM (Atm), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
ATM Proteins for Sus scrofa (Pig)
Code | Product Name | Source |
---|---|---|
CSB-YP754283PI CSB-EP754283PI CSB-BP754283PI CSB-MP754283PI CSB-EP754283PI-B |
Recombinant Pig Serine-protein kinase ATM (ATM), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
ATM Proteins for Arabidopsis thaliana (Mouse-ear cress)
Code | Product Name | Source |
---|---|---|
CSB-YP885532DOA CSB-EP885532DOA CSB-BP885532DOA CSB-MP885532DOA CSB-EP885532DOA-B |
Recombinant Arabidopsis thaliana Serine/threonine-protein kinase ATM (ATM), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |