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The vectors expressing anti-ATM antibody were constructed as follows: immunizing an animal with a synthesized peptide derived from human Phospho-ATM (S1981), isolating the positive splenocyte and extracting RNA, obtaining DNA by reverse transcription, sequencing and screening ATM antibody gene, and amplifying heavy and light chain sequence by PCR and cloning them into plasma vectors. After that, the vector clones were transfected into the mammalian cells for production. The product is the recombinant ATM antibody. Recombinant ATM antibody in the culture medium was purified using affinity-chromatography. It can react with ATM protein from Human and is used in the ELISA, IF.
ATM is a protein-coding gene that encodes the serine-protein kinase ATM. Diseases associated with ATM include ataxia and mantle cell lymphoma. Its related pathways include miRNA regulation of DNA damage response and DNA damage response. According to some research, ATM may have the following features.
ATM is an important sensor of reactive oxygen species in human cells. A portion of nuclear ATM co-localizes with γ-H2AX at DSBs in response to DNA damage. ATM is also active in other cellular signaling pathways involved in maintaining cellular homeostasis. Various injury-induced responses may be activated by enhancing the protein kinase activity of ATM. ATM is activated by DNA double-strand breaks through the Mre11-Rad50-Nbs1 complex. ATM and ATR often work together to signal DNA damage and regulate downstream processes.
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