Acetyl-Histone H3.1(K4)Antibody

Datasheet
Code CSB-RA010418A04acHU
Size US$350
Uniprot No. P68431
Image
  • Western Blot
    Positive WB detected in:Hela whole cell lysate treated by 15mM sodium butyrate for 30min
    All lanes:Acetyl-Histone H3.1(K4)antibody at 1.1μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 15 KDa
    Observed band size: 15 KDa

  • Immunocytochemistry analysis of CSB-RA010418A04acHU diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of Hela cells(treated by 15mM sodium butyrate for 30min) with CSB-RA010418A04acHU at 1:68,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunogen A synthesized peptide
Species Reactivity Human
Tested Applications ELISA, WB, ICC, IF; Recommended dilution: WB:1:500-1:2000, ICC:1:50-1:500, IF:1:30-1:200
Relevance Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Alias Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3A, H3FA, AND, HIST1H3B, H3FL, AND, HIST1H3C, H3FC, AND, HIST1H3D, H3FB, AND, HIST1H3E, H3FD, AND, HIST1H3F, H3FI, AND, HIST1H3G, H3FH, AND, HIST1H3H, H3FK, AND, HIST1H3I, H3FF, AND, HIST1H3J, H3FJ
Immunogen Species Homo sapiens (Human)
Research Area Epigenetics and Nuclear Signaling
Gene Names HIST1H3A
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Involvement in disease Glioma (GLM)
Subcellular Location Nucleus, Chromosome
Protein Families Histone H3 family
Database Links

HGNC: 4766

OMIM: 137800

KEGG: hsa:8350

STRING: 9606.ENSP00000444823

UniGene: Hs.132854

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