Code | CSB-RA131953A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
IHC | 1:50-1:200 |
FC | 1:50-1:200 |
To produce a recombinant monoclonal antibody against BCL6, CUSABIO initiated the process by immunizing a rabbit with a synthesized peptide derived from human BCL6 protein. Following immunization, B cells were isolated from the rabbit, and RNA was extracted from these cells. The extracted RNA was reverse-transcribed into cDNA, which was used as a template to extend BCL6 antibody genes using degenerate primers. These extended BCL6 antibody genes were then incorporated into a plasmid vector and transfected into host cells for expression. Subsequently, the BCL6 recombinant monoclonal antibody was purified from the cell culture supernatant through affinity chromatography and evaluated for its suitability in ELISA, IHC, and FC applications, demonstrating specific recognition of human BCL6 protein.
BCL6 is a critical transcriptional repressor that regulates B cell development, germinal center formation, and the adaptive immune response. Its functions are essential for generating high-affinity antibodies and maintaining the balance between immune response and immune tolerance. Dysregulation of BCL6 can have significant implications for B cell lymphomagenesis and immune-related diseases.
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