C1S Recombinant Monoclonal Antibody

C1S serves as a critical serine protease within the classical complement pathway, functioning as part of the C1 complex that initiates immune responses against pathogens and altered self-cells. This protein plays essential roles in both protective immunity and pathological conditions, making it a significant target for researchers investigating complement-mediated diseases, autoimmune disorders, and inflammatory processes.

This recombinant monoclonal antibody against human C1S offers the consistency and reliability that demanding experimental workflows require. Developed using recombinant technology with clone 12F12, this antibody provides sequence-defined specificity that eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived reagents. The human IgG1 isotype format, combined with affinity chromatography purification, ensures high purity suitable for sensitive detection methods.

Validation studies demonstrate effective performance in immunohistochemistry applications using human liver tissue sections. Testing on a Leica Bond system with citrate buffer antigen retrieval at pH 6.0 and overnight primary antibody incubation at 4°C produced clear, specific staining at 1:100 dilution, with a recommended working range of 1:50 to 1:200 for IHC applications. The antibody is also validated for ELISA, providing flexibility across different experimental platforms.

Supplied in a liquid format containing 50% glycerol and PBS at pH 7.4 with 0.03% Proclin 300 as preservative, this antibody maintains stability when stored at -20°C or -80°C while avoiding repeated freeze-thaw cycles. Researchers investigating complement biology, cardiovascular disease mechanisms, or immunological pathways will find this antibody a dependable tool for characterizing C1S expression and distribution in human tissues.