Product Details

Uniprot No.

Target Names

Alternative Names

Caspase-5 (CASP-5) (EC 3.4.22.58) (ICE(rel)-III) (Protease ICH-3) (Protease TY) [Cleaved into: Caspase-5 subunit p20, Caspase-5 subunit p10], CASP5, ICH3

Species Reactivity

Human

Immunogen

A synthesized peptide from human CASP5 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9B2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, FC

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Protocols

ELISA Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Caspase-5 plays a central role in inflammatory signaling pathways, functioning as a key component of the inflammasome complex where it processes pro-inflammatory cytokines and contributes to pyroptotic cell death. This inflammatory caspase has garnered significant research attention for its involvement in innate immune responses, particularly in the context of bacterial infection sensing and inflammatory disease pathogenesis. Understanding CASP5 activity and expression patterns provides valuable insights into mechanisms underlying conditions ranging from sepsis to inflammatory bowel disease.

This recombinant monoclonal antibody, clone 9B2, offers researchers the reproducibility advantages inherent to recombinant technology. Because the antibody sequence is defined and produced from a stable expression system, you can expect consistent performance across experiments and over time, eliminating the lot-to-lot variability that can complicate long-term studies or multi-site collaborations. The rabbit IgG format, purified by affinity chromatography, ensures high specificity for your target.

Validation for flow cytometry applications has been performed using HepG2 cells, demonstrating clear separation between specific staining and isotype control populations. The recommended working dilution range of 1:50 to 1:200 for flow cytometry provides flexibility to optimize signal intensity based on your specific experimental conditions and instrument sensitivity. The antibody has also been validated for ELISA applications, supporting quantitative detection workflows.

Supplied in a glycerol-containing buffer optimized for long-term stability at -20°C or -80°C, this antibody is well-suited for researchers investigating inflammasome biology, inflammatory caspase cascades, or pyroptosis mechanisms in human cell systems. Its defined specificity for human CASP5 makes it a reliable tool for characterizing inflammatory pathway activation in disease-relevant models.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA224345A0HU
Size	US$210
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Image	Overlay Peak curve showing HepG2 cells stained with CSB-RA224345A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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CASP5 Recombinant Monoclonal Antibody

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