Product Details

Uniprot No.

P20248

Target Names

Alternative Names

CCN1 antibody; CCNA antibody; Ccna2 antibody; CCNA2_HUMAN antibody; Cyclin A2 antibody; Cyclin-A antibody; Cyclin-A2 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human CCNA2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8A8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IF, FC

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Cyclin A2 plays a central role in cell cycle progression, functioning as a key regulatory subunit that activates cyclin-dependent kinases during both S phase DNA replication and the G2/M transition. Its tightly controlled expression pattern—accumulating through S phase and degrading at prometaphase—makes it an essential marker for studying cell proliferation, checkpoint regulation, and the molecular mechanisms underlying uncontrolled cell division in cancer research.

This recombinant monoclonal antibody targeting human CCNA2 offers the reproducibility that demanding cell cycle studies require. Developed using recombinant technology with clone 8A8, the antibody provides sequence-defined consistency between lots, eliminating the variability that can complicate longitudinal studies or multi-site collaborations. The rabbit IgG format, raised against a synthetic peptide derived from human CCNA2, ensures defined epitope specificity for your experiments.

Validation in immunofluorescence demonstrates clear nuclear localization in HeLa cells, with effective staining achieved at dilutions between 1:50 and 1:200. The characteristic nuclear signal aligns with Cyclin A2's known localization during active cell cycling. Flow cytometry validation, also performed in HeLa cells, confirms the antibody's utility for quantitative single-cell analysis, showing distinct positive population shifts compared to isotype controls at 1:100 dilution. This dual validation in both imaging and cytometric applications provides flexibility for researchers examining cell cycle dynamics through complementary approaches.

Whether you're investigating proliferation markers in cancer cell lines, characterizing cell cycle synchronization protocols, or examining checkpoint responses to genotoxic stress, this antibody supports robust detection of Cyclin A2 in human samples across multiple experimental platforms.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. Functions through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 or CDK2. The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle.

Gene References into Functions

High CCNA2 expression is associated with hepatocellular carcinoma progression. PMID: 30106440
Study demonstrates that cyclin A2 interacts with actin and RhoA during mitosis, and that cyclin A2 depletion induces a dramatic decrease in active RhoA in mitosis suggesting that cyclin A2 participation in RhoA activation in late mitosis. PMID: 27279564
Bisphosphonate inhibits the expression of cyclin A2 at the transcriptional level in normal human oral keratinocytes. PMID: 28713904
the presented evidence suggested that WA can inhibit HCC cell proliferationand tumorigenesis through miR-22-repressed CCNA2, which was at least partially through FXR regulation PMID: 27738335
Findings suggest that ERK1/2-mediated Cdk2/cyclin A signaling pathway is involved in 7-hydroxy-5,4'-dimethoxy-2-arylbenzofuran (Ary) - induced G1/S-phase arrest. PMID: 27259234
some cancer cells are defective for efficient interaction between p27 and CycA-CDK complex due to qualitative alteration(s) PMID: 29098944
This study shows that cocaine induces significant increases in both the astrocytic expression of cyclin A2 and the proliferation of primary human astrocytes. PMID: 27834787
Low CCNA expression is associated with lung cancer progression. PMID: 27694898
inhibition of PDK4 activity in Hepatocellular carcinoma cells increased cyclin E1, cyclin A2, and E2F1 proteins. PMID: 28003426
An increase of cyclin A2 expression was identified for the first time in a case of splenic diffuse red pulp small B-cell lymphoma. PMID: 27761608
Consistent with these findings, a genome-scale pooled RNA interference screen revealed that toxic doses of MK-1775 are suppressed by CDK2 or Cyclin A2 knockdown. These findings support G2 exit as the more significant effect of Wee1 inhibition in pancreatic cancers. PMID: 26890070
Clioquinol suppressed cell cycle progression in the S-phase in SMMC-7721 hepatoma cells via the p21, p27-cyclin E,A/Cdk2 pathway. PMID: 26677902
On analyzing cyclin A and B1 (CCNA and CCNB1) expression, positive staining in 90% cases of PTC were observed. The study revealed a significant difference in expression of cyclins A and B1 between classic and non-classic variants of PTC. PMID: 26706989
Study describes a positive feedback loop centred on cyclin A2-Cdk2 inhibition of interphase APC/C-Cdc20 to allow further cyclin A2 accumulation and mitotic entry. PMID: 26960431
PIWIL2 has a role in promoting progression of non-small cell lung cancer by inducing CDK2 and Cyclin A expression PMID: 26373553
Cyclin A2-binding function of pUL21a contributes to the maintenance of a cell cycle state conducive for the completion of the human cytomegalovirus (HCMV) replication cycle. PMID: 25393019
HDAC10 regulates cyclin A2 expression by deacetylating histones near the let-7 promoter. PMID: 26240284
Results suggest that cyclin A2 overexpression may drive cancer by promoting AKT-dependent proliferation and survival. PMID: 24795023
for the first time, we demonstrated that CTD induced G2/M phase arrest via the inhibition of Cdc25c and cyclin A and induced apoptosis was through death receptor (extrinsic), intrinsic (mitochondria) and ER stress pathways in A375. S2 cells. PMID: 25340978
CCN2 is a regulator of the transition through cytokinesis during terminal erythropoiesis. PMID: 25615569
CCNA2 is a biomarker for the prognosis of ER+ breast cancer and monitoring of tamoxifen efficacy PMID: 24622579
our data showed that bile acid-activated FXR stimulates miR-22-silenced CCNA2, a novel pathway for FXR to exert its protective effect in the gastrointestinal tract. PMID: 25596928
Expression of cycle marker cyclin A1 differs between benign and malignant papillary breast lesions. PMID: 25501285
Cyclin A2 and its associated kinase (cdk2) activity are required for optimal induction of progesterone receptor target genes in breast cancer cells. PMID: 25220500
It is concluded that non-response to everolimus is characterized by increased cdk2/cyclin A, driving RCC cells into the G2/M-phase. VPA hinders everolimus non-response by diminishing cdk2/cyclin A PMID: 24935000
cyclin A expression predicted 100% of the patients at risk for metastasis of rectal neuroendocrine tumors. PMID: 24824027
The aim of the present study was to evaluate the prognostic value of the proliferation factors mitotic activity index (MAI), phosphohistone H3 (PPH3), cyclin B1, cyclin A and Ki67, alone and in combinations. PMID: 24324728
show that cyclin A is an independent prognostic factor in endometrioid adenocarcinoma PMID: 24519066
level unchanged in endometrial cancer PMID: 23815208
APPOLON to be a novel regulator of mitotic CYCLIN A degradation independent of SAC. PMID: 24302728
Data indicate that the major tegument 150-kDa phosphoprotein (pp150) of human cytomegalovirus (HCMV) binds to cyclin A2 via a functional RXL/Cy motif resulting in its cyclin A2-dependent phosphorylation. PMID: 24101496
HDAC3 regulates cyclin A stability. PMID: 23760262
Cyclin A creates a cellular environment that promotes microtubule detachment from kinetochores in prometaphase to ensure efficient error correction and faithful chromosome segregation PMID: 24013174
We further detected that reduced level of GRK2 induced a small cell cycle arrest at G2/M phase by enhancing the expression of cyclin A PMID: 23460259
Overexpression of cyclin A2 is associated with stage I non-small cell lung cancer. PMID: 23115005
human papillomavirus E4 proteins can interact with cyclin A and cdk2, which may contribute to viral manipulation of the host cell cycle. PMID: 23065011
The expression of cell proliferation markers cyclin A and p27 are independent prognostic factors in patients with esophageal squamous cell carcinoma. PMID: 21762277
The study has revealed that some highly differentiated human tissues express an intron-retaining cyclin A2 mRNA that induced a G1/S block in vitro. PMID: 22745723
Human cytomegalovirus IE1/2 expression was downregulated by cyclin A2, CDK1 and CDK2. PMID: 22718829
cyclin A and cyclin B1 are useful markers in the distinction of benign and malignant thyroid tumors PMID: 21264543
GC box was responsible for the cyclic activity of human CKAP2 promoter through the phosphorylation of Sp1, possibly by Cyclin A/Cdk complex. PMID: 22465120
Data demonstrated that in addition to galectin-3, HK III and cyclin A profiles could be important biomarkers in predicting malignancy in follicular thyroid nodules. PMID: 17868400
Cyclin A2 negatively controls cell motility by promoting RhoA activation, thus demonstrating a novel Cyclin A2 function in cytoskeletal rearrangements and cell migration. PMID: 22232705
NF-Y binds to CCAAT sequences in the Cyclin A promoter, as well as to those in the promoters of cell cycle G2 regulators such as CDC2, Cyclin B and CDC25C. PMID: 21871181
These data demonstrate that B55alpha acts to antagonize Cyclin A/Cdk-dependent activation of FoxM1, to ensure that FoxM1 activity is restricted to the G(2) phase of the cell cycle. PMID: 21813648
The current results suggested that an SNP (rs769236) at the promoter of CCNA2 may be associated significantly with increased risk of colon, liver, and lung cancers. PMID: 21858804
The expression of mRNA and protein of Cyclin A in hypertrophic scar changes from high level to low level as the hypertrophic scar develops, while the expression of P21cip1 changes from low level to high level. PMID: 21046779
Low p16(Ink4a) expression, overexpression of p53, cytoplasmic p27(Kip1), and Cyclin A are predictive markers for shorter overall survival in ovarian carcinomas. PMID: 21464733
cyclin A was strongly associated with the expression of cyclin E in invasive ductal breast carcinoma PMID: 21472690
Cdc20 requires APC3 and APC8 to bind and activate the APC/C when the spindle assembly checkpoint is satisfied, but only APC8 when active, and APC10 is crucial for the destruction of cyclin B1 and securin, but not cyclin A PMID: 21336306

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Subcellular Location

Nucleus. Cytoplasm.

Protein Families

Cyclin family, Cyclin AB subfamily

Database Links

HGNC: 1578

OMIM: 123835

KEGG: hsa:890

STRING: 9606.ENSP00000274026

UniGene: Hs.58974

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Code	CSB-RA926576A0HU
Size	US$210
	Order now
Image	Immunofluorescence staining of HeLa cell with CSB-RA926576A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA926576A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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CCNA2 Recombinant Monoclonal Antibody

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