Code | CSB-RA934881A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
IP | 1:200-1:1000 |
CUSABIO immunized an animal with a synthesized peptide derived from human CD8 to stimulate an immune response. B cells were isolated from the immunized animal and fused with myeloma cells to form hybridoma cells. A single hybridoma cell clone that produces CD8A-specific antibodies was screened and selected. RNA was extracted from the chosen hybridoma cells, and the gene sequence encoding the CD8A antibody was amplified using reverse transcription PCR. These CD8A antibody genes were then cloned into an expression vector and transfected into a host system for expression. The CD8A recombinant monoclonal antibodies were purified from the cell culture supernatant using affinity chromatography. The binding specificity and affinity of the recombinant CD8A monoclonal antibody were confirmed using four applications including ELISA, WB, IHC, and IP. This antibody specifically recognizes human CD8A protein.
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