CD8A

CD8A encodes the CD8 alpha chain protein, also commonly known as T-cell surface glycoprotein CD8 alpha chain. This protein is a crucial component of the CD8 complex on the surface of cytotoxic T lymphocytes. It binds to major histocompatibility complex (MHC) class I molecules, facilitating T-cell recognition and activation. It is involved in the T-cell receptor signaling pathway.
Dysregulation of CD8A - related functions is associated with various diseases, such as cancer and autoimmune disorders. In cancer immunotherapy, targeting CD8 - related pathways has shown promise. There are ongoing efforts in drug development to enhance the anti - tumor activity of CD8 + T cells, aiming to improve patient outcomes.

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CD8A at 5 μg/ml can bind Anti-CD8A recombinant antibody (CSB-RA004966MA3HU). The EC₅₀ is 11.49-20.78 ng/mL.

Recombinant Human T-cell surface glycoprotein CD8 alpha chain (CD8A), partial (CSB-YP004966HU)

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP004966HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) CD8A.

CD8A Recombinant Monoclonal Antibody (CSB-RA934881A0HU)

Validated Data

Western Blot
Positive WB detected in: MCF-7 whole cell lysate, THP-1 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate
All lanes: CD8A antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 26, 22, 31 kDa
Observed band size: 31 kDa

IHC image of CSB-RA934881A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA934881A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Immunoprecipitating CD8A in HepG2 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA934881A0HU in HepG2 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2:CSB-RA934881A0HU(3µg)+ HepG2 whole cell lysate(500µg)
Lane 3: HepG2 whole cell lysate (10µg)

Human Cluster of differentiation 8,CD8 ELISA Kit (CSB-E12707h)

Validated Data

Code: CSB-E12707h

Size: 96T,5×96T,10×96T

Sensitivity: 15.6 U/mL

Detection Range: 62.5 U/mL-4000 U/mL

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

The following CD8A reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

CD8A Antibodies

CD8A Proteins

CD8A ELISA Kits

CD8A Antibodies

CD8A Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA004966LA01HU	CD8A Antibody	Human	ELISA, IHC
CSB-PA004966LC01HU	CD8A Antibody, FITC conjugated	Human
CSB-PA004966LD01HU	CD8A Antibody, Biotin conjugated	Human	ELISA
CSB-RA934881A0HU	CD8A Recombinant Monoclonal Antibody	Human	ELISA, WB, IHC, IP

CD8A Antibodies for Bos taurus (Bovine)

Code	Product Name	Species Reactivity	Application
CSB-PA004966ZA01BO	CD8A Antibody	Bos taurus	ELISA, WB (ensure identification of antigen)

CD8A Proteins

CD8A Proteins for Bos taurus (Bovine)

Code	Product Name	Source
CSB-YP004966BO	Recombinant Bovine T-cell surface glycoprotein CD8 alpha chain (CD8A), partial	Yeast
CSB-EP004966BO	Recombinant Bovine T-cell surface glycoprotein CD8 alpha chain (CD8A), partial	E.coli
CSB-BP004966BO CSB-MP004966BO CSB-EP004966BO-B	Recombinant Bovine T-cell surface glycoprotein CD8 alpha chain (CD8A)	Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD8A Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP004966HU	Recombinant Human T-cell surface glycoprotein CD8 alpha chain (CD8A), partial	Yeast
CSB-EP004966HU	Recombinant Human T-cell surface glycoprotein CD8 alpha chain (CD8A), partial	E.coli
CSB-MP004966HU	Recombinant Human T-cell surface glycoprotein CD8 alpha chain (CD8A), partial	Mammalian cell
CSB-BP004966HU CSB-EP004966HU-B	Recombinant Human T-cell surface glycoprotein CD8 alpha chain (CD8A), partial	Baculovirus In Vivo Biotinylation in E.coli
CSB-YP004966HUf3	Recombinant Human T-cell surface glycoprotein CD8 alpha chain(CD8A), partial	Yeast
CSB-MP004966HUd7	Recombinant Human T-cell surface glycoprotein CD8 alpha chain (CD8A), partial (Active)	Mammalian cell

CD8A Proteins for Pongo pygmaeus (Bornean orangutan)

Code	Product Name	Source
CSB-CF004966EXP	Recombinant Pongo pygmaeus T-cell surface glycoprotein CD8 alpha chain (CD8A)	in vitro E.coli expression system
CSB-YP004966EXP1 CSB-EP004966EXP1 CSB-BP004966EXP1 CSB-MP004966EXP1 CSB-EP004966EXP1-B	Recombinant Pongo pygmaeus T-cell surface glycoprotein CD8 alpha chain (CD8A), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD8A Proteins for Rattus norvegicus (Rat)

Code	Product Name	Source
CSB-CF004966RA	Recombinant Rat T-cell surface glycoprotein CD8 alpha chain (Cd8a)	in vitro E.coli expression system
CSB-YP004966RA1 CSB-EP004966RA1 CSB-BP004966RA1 CSB-MP004966RA1 CSB-EP004966RA1-B	Recombinant Rat T-cell surface glycoprotein CD8 alpha chain (Cd8a), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD8A Proteins for Canis lupus familiaris (Dog) (Canis familiaris)

Code	Product Name	Source
CSB-CF004966DO	Recombinant Dog T-cell surface glycoprotein CD8 alpha chain (CD8A)	in vitro E.coli expression system
CSB-YP004966DO1 CSB-BP004966DO1 CSB-MP004966DO1 CSB-EP004966DO1-B	Recombinant Dog T-cell surface glycoprotein CD8 alpha chain (CD8A), partial	Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD8A Proteins for Felis catus (Cat) (Felis silvestris catus)

Code	Product Name	Source
CSB-CF004966CA	Recombinant Cat T-cell surface glycoprotein CD8 alpha chain (CD8A)	in vitro E.coli expression system
CSB-YP004966CA1 CSB-EP004966CA1 CSB-BP004966CA1 CSB-MP004966CA1 CSB-EP004966CA1-B	Recombinant Cat T-cell surface glycoprotein CD8 alpha chain (CD8A), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD8A Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-CF004966MO	Recombinant Mouse T-cell surface glycoprotein CD8 alpha chain (Cd8a)	in vitro E.coli expression system
CSB-YP004966MO1 CSB-EP004966MO1 CSB-BP004966MO1 CSB-MP004966MO1 CSB-EP004966MO1-B	Recombinant Mouse T-cell surface glycoprotein CD8 alpha chain (Cd8a), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD8A ELISA Kit

CD8A ELISA Kit for Homo sapiens (Human)

Code	Product Name	Sample Types	Sensitivity
CSB-E12707h	Human Cluster of differentiation 8,CD8 ELISA Kit	serum, plasma, cell culture supernates, tissue homogenates	15.6 U/mL

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CD8A

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