CLEC5A Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA873686MA1HU
Size	US$210
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Image	The Binding Activity of Human CLEC5A with Anti-CLEC5A Recombinant Antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CLEC5A (CSB-MP873686HU) at 2 μg/mL can bind Anti-CLEC5A recombinant antibody. The EC50 is 0.5964-0.6743 ng/mL. The Binding Activity of Human CLEC5A with Anti-CLEC5A Recombinant Antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CLEC5A (CSB-MP873686HUi4-B) at 2 μg/mL can bind Anti-CLEC5A recombinant antibody. The EC50 is 0.3246-0.4731 ng/mL. The Binding Activity of Human CLEC5A with Anti-CLEC5A Recombinant Antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CLEC5A (CSB-MP873686HUi4-B) at 2 μg/mL can bind Anti-CLEC5A recombinant antibody. The EC50 is 1.553-1.763 ng/mL. The Binding Activity of Human CLEC5A with Anti-CLEC5A Recombinant Antibody Activity: CLEC5A Recombinant Monoclonal Antibody captured on Protein A Chip can bind Recombinant Human CLEC5A (CSB-MP873686HU) with an affinity constant of 0.285 nM as detected by MetaSPR Assay (WeSPRTM 200). The Binding Activity of Human CLEC5A with Anti-CLEC5A Recombinant Antibody Activity: CLEC5A Recombinant Monoclonal Antibody captured on Protein A Chip can bind Recombinant Human CLEC5A (CSB-MP873686HUi4-B) with an affinity constant of 0.444 nM as detected by MetaSPR Assay (WeSPRTM 200). Untransfected CHOK1*10⁶cells), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
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Product Details
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Target Background

Product Details

Uniprot No.

Q9NY25

Target Names

CLEC5A

Alternative Names

C-type lectin domain family 5 member A (C-type lectin superfamily member 5) (Myeloid DAP12-associating lectin 1) (MDL-1), CLEC5A, CLECSF5 MDL1

Species Reactivity

Human

Immunogen

Recombinant Human CLEC5A protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

22D7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, FC, SPR

Recommended Dilution

Application	Recommended Dilution
FC	1:20-1:200

Protocols

ELISA Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Functions as a positive regulator of osteoclastogenesis. Cell surface receptor that signals via TYROBP. Regulates inflammatory responses.; (Microbial infection) Critical macrophage receptor for dengue virus serotypes 1-4. The binding of dengue virus to CLEC5A triggers signaling through the phosphorylation of TYROBP. This interaction does not result in viral entry, but stimulates proinflammatory cytokine release.

Gene References into Functions

MDL-1 is mainly expressed in atherosclerotic lesional macrophages and increased macrophage MDL-1 expression is associated with early plaque progression and promotes macrophage survival. PMID: 29126450
this study shows association of rs1285933 single nucleotide polymorphism in CLEC5A gene with dengue severity PMID: 28764923
CLEC5A is critical for dengue virus-induced osteoclast activation and bone homeostasis PMID: 27033255
Mannose Receptor and CLEC5A on Macrophage first attracts the Dengue Virus with high avidity, and the virus concurrently interacts with CLEC5A in close proximity to form a multivalent hetero-complex and facilitate CLEC5A-mediated signal transduction. Dengue Virus Infection Is through a Cooperative Interaction between a Mannose Receptor and CLEC5A on Macrophage as a Multivalent Hetero-Complex. PMID: 27832191
The results suggest that CLEC5A-mediated enhancement of the inflammatory response in myeloid cells contributes to influenza pathogenicity in vivo and may be considered a therapeutic target in combination with effective antivirals. PMID: 27795434
Concordant overexpression of MDL-1 and DAP12 were correlated with increased production of proinflammatory cytokines in rheumatoid arthritis patients. PMID: 24465901
Blockade of CLEC5A inhibits NLRP3 inflammasome activation and pyrotopsis in dengue-virus-infected inflammatory macrophages. Thus, DV can activate NLRP3 inflammasome via CLEC5A. PMID: 23152543
No significant associations were noted between the genotypes and allele frequency of the 4 CLEC5A tSNPs between controls and Kawasaki disease patients. PMID: 22536019
CLEC5A is homodimeric at the cell surface and binds to dengue virus serotypes 1-4. PMID: 21566123
CLEC5A expression in monocyte/macrophage and granulocytes is regulated by PU.1. PMID: 21094529
The crystal volume per unit weight (VM) was calculated to be 2.34 A3 Da-1, with nine molecules per asymmetric unit and a solvent content of 47.38%. CLEC5A plays an important role in the pathophysiology of dengue-virus-induced disease. PMID: 20057064
blockade of CLEC5A-mediated signalling attenuates the production of proinflammatory cytokines by macrophages infected with Dengue virus (either alone or complexed with an enhancing antibody) PMID: 18496526

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Subcellular Location

Cell membrane; Single-pass type II membrane protein.

Tissue Specificity

Highly expressed in bone marrow with lower levels in synovium, lung and bronchus. Expressed in peripheral blood monocytes and in the monocyte/macrophage cell lines U-937 and Mono-Mac-6, but not in cell lines of other origins. Expression is down-regulated

Database Links

HGNC: 2054

OMIM: 604987

KEGG: hsa:23601

STRING: 9606.ENSP00000449999

UniGene: Hs.446235

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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